陕北地区提高石油采收率菌株筛选及其降解性能评价
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摘要
筛选出可提高石油采收率的降解菌株。采用富集培养和稀释平板法,从陕北某采油厂附近长期受原油污染的水域和油污泥中筛选获得以原油为唯一碳源的降油菌株,并对其生长条件、排油圈能力、代谢产物表面活性物质、表面张力与乳化性及原油降解实验进行一系列的研究。含油废水和油污泥中筛选出10株细菌,从中选择了6株优势菌作为供试菌株进行后续研究,6株供试菌株均可使原油的理化性质发生变化。菌株B与原油作用后,使原油黏度降低了49.02%,菌株A-08、菌株5-13、菌株1-2及菌株A和菌株A-08复合菌群可使原油乳化为细小的圆型颗粒,与原油作用后,降解率达到43.25%-60.00%。
In order to screen the strains that can enhance oil recovery by using metabolites,using enrichment and dilution plate method,strains of degrading crude oil as sole carbon source were screened from nearby long-time petroleum-polluted waters and oily sludge in the northern Shaanxi,and their characteristics,including their growth condition,oil displacement capability,surface active substance of metabolites,surface tension and emulsification,and the degradation of crude oil were analyzed,respectively. Ten strains of bacteria were screened from wastewater containing oil and oily sludge. Further,6 dominant bacteria were selected as test ones for follow-up study,6 strains of bacteria resulted in the physicochemical properties of crude oil changed. Crude oil viscosity reduced by 49.02% after strain B and crude oil reacted,the composite bacteria of strain A-08,strain 5-13,strain 1-2,strain A,and strain A-08 the crude oil emulsified into circular small particles,and the degradation rate reached 43.25%-60.00%.
In order to screen the strains that can enhance oil recovery by using metabolites,using enrichment and dilution plate method,strains of degrading crude oil as sole carbon source were screened from nearby long-time petroleum-polluted waters and oily sludge in the northern Shaanxi,and their characteristics,including their growth condition,oil displacement capability,surface active substance of metabolites,surface tension and emulsification,and the degradation of crude oil were analyzed,respectively. Ten strains of bacteria were screened from wastewater containing oil and oily sludge. Further,6 dominant bacteria were selected as test ones for follow-up study,6 strains of bacteria resulted in the physicochemical properties of crude oil changed. Crude oil viscosity reduced by 49.02% after strain B and crude oil reacted,the composite bacteria of strain A-08,strain 5-13,strain 1-2,strain A,and strain A-08 the crude oil emulsified into circular small particles,and the degradation rate reached 43.25%-60.00%.
引文
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[10]邓振山,马琳,张袭,等.一株产表面活性剂石油降解菌株筛选及其特性[J].环境工程学报, 2017, 11(5):3295-3303.
[11]邓振山,高飞,刘玉珍,等.基于含蜡状芽孢杆菌的生物刺激-生物强化联合体系降解石油污染物[J].南华大学学报:自然科学版, 2018, 32(2):19-25.
[12]邓振山,张袭,占鹏.玫瑰色微球菌A-04的红色素鉴定及稳定性分析[J].天然产物开发与研究, 2018. doi:http://kns.cnki. net/kcms/detail/51. 1335. Q. 20180703. 0958. 008. html.
[13]姚瑶,刘兆普,等.胜利油田土壤中石油降解菌分离和鉴定及代谢产物的研究[J].南京农业大学学报, 2013, 36(1):65-71.
[14]邓丽萍,蒋新兴,焦龙进.微生物采油菌种性能评价的研究方法[J].内蒙古石油化工, 2012(12):9-10.
[15]张俊会.几株真菌和细菌对原油理化性质的影响[D].杨凌:西北农林科技大学, 2012.
[16]左珊珊,吴娟.生物表面活性剂合成条件的优化及提取方法[J].环境工程学报, 2016,(6):3325-3329.
[17]Yin H, Qiang J, Jia Y, et al. Characteristics of biosurfactant produced by Pseudomonas aeruginosa S6 isolated from oil-containing wastewater[J]. Process Biochemistry, 2009, 44(3):302-308.
[18]吴涛,谢文军,依艳丽,等.耐盐石油烃降解菌的筛选鉴定及其特性研究[J].环境科学, 2012, 33(11):3949-3955.
[19]吴玉新.紫外分光光度计法测定污水中含油量的研究[J].环境保护, 1998.
[20]黄曼曼,邓百万,等.机油高效降解菌的筛选鉴定及降解特性的初步研究[J].生物技术通报, 2018, 34(6):155-163.
[21]张娜,隆小华,刘兆普,等.石油降解菌x-1产表面活性剂的研究[J].天然产物研究与开发, 2012, 24(3):389-392.
[22]单金玉,贾莹,刘健,等.两株假单胞菌对烃的作用及其协同效应[J].微生物学报, 2002, 29(4):55-58.
[23]杨慧娟,刘五星,骆永明,等.气相色谱-质谱法分段测定土壤中的可取总石油烃[J].土壤, 2014, 46(1):134-138.
[24]姜岩,伍涛,张贤明.土壤中石油烃预处理及含量分析方法研究进展[J].土壤(Soils), 2015, 47(3):461-465.
[25]张胜祥,张玉辉,许德军.摇瓶发酵烃降解率测定方法的研究[J].郑州师范教育, 2013, 2(2):43-45.
[2]李茂毅.石油烃降解菌株的选育以及对石油中烃类物质降解的研究[D].西安:西北大学, 2011.
[3]Mulligan CN. Environmental applications for biosurfactants[J].Environmental Pollution, 2005, 133(2):183-198.
[4]CameotraSS,SinghP.Bioremediationofoilsludgeusing crude biosurfactants[J]. International Biodeterioration and Biodegradation, 2008, 62(3):274-280.
[5]赵栋,张洋,王玲,刘婷.微生物采油技术综述[J].内江科技,2013(6):150.
[6]张俊会,王平,薛泉宏,等.延长石油增油细菌的筛选及其降解特性的研究[J].西北农林大学学报:自然科学版, 2012, 40(10):120-128.
[7]撕拉依力·托合提.复合微生物降解原油及其馏分研究[D].南京:南京理工大学, 2009, 19(1):21-23.
[8]韩寒冰.原油降解菌的筛选及其降解性能的研究[J].茂名学院学报, 2009, 19(1):21-23.
[9]刘涛,宋智勇,曹功泽,等.中一区Ng3区块微生物驱油物模实验研究[J].应用与环境生物学报, 2013, 19(2):335-341.
[10]邓振山,马琳,张袭,等.一株产表面活性剂石油降解菌株筛选及其特性[J].环境工程学报, 2017, 11(5):3295-3303.
[11]邓振山,高飞,刘玉珍,等.基于含蜡状芽孢杆菌的生物刺激-生物强化联合体系降解石油污染物[J].南华大学学报:自然科学版, 2018, 32(2):19-25.
[12]邓振山,张袭,占鹏.玫瑰色微球菌A-04的红色素鉴定及稳定性分析[J].天然产物开发与研究, 2018. doi:http://kns.cnki. net/kcms/detail/51. 1335. Q. 20180703. 0958. 008. html.
[13]姚瑶,刘兆普,等.胜利油田土壤中石油降解菌分离和鉴定及代谢产物的研究[J].南京农业大学学报, 2013, 36(1):65-71.
[14]邓丽萍,蒋新兴,焦龙进.微生物采油菌种性能评价的研究方法[J].内蒙古石油化工, 2012(12):9-10.
[15]张俊会.几株真菌和细菌对原油理化性质的影响[D].杨凌:西北农林科技大学, 2012.
[16]左珊珊,吴娟.生物表面活性剂合成条件的优化及提取方法[J].环境工程学报, 2016,(6):3325-3329.
[17]Yin H, Qiang J, Jia Y, et al. Characteristics of biosurfactant produced by Pseudomonas aeruginosa S6 isolated from oil-containing wastewater[J]. Process Biochemistry, 2009, 44(3):302-308.
[18]吴涛,谢文军,依艳丽,等.耐盐石油烃降解菌的筛选鉴定及其特性研究[J].环境科学, 2012, 33(11):3949-3955.
[19]吴玉新.紫外分光光度计法测定污水中含油量的研究[J].环境保护, 1998.
[20]黄曼曼,邓百万,等.机油高效降解菌的筛选鉴定及降解特性的初步研究[J].生物技术通报, 2018, 34(6):155-163.
[21]张娜,隆小华,刘兆普,等.石油降解菌x-1产表面活性剂的研究[J].天然产物研究与开发, 2012, 24(3):389-392.
[22]单金玉,贾莹,刘健,等.两株假单胞菌对烃的作用及其协同效应[J].微生物学报, 2002, 29(4):55-58.
[23]杨慧娟,刘五星,骆永明,等.气相色谱-质谱法分段测定土壤中的可取总石油烃[J].土壤, 2014, 46(1):134-138.
[24]姜岩,伍涛,张贤明.土壤中石油烃预处理及含量分析方法研究进展[J].土壤(Soils), 2015, 47(3):461-465.
[25]张胜祥,张玉辉,许德军.摇瓶发酵烃降解率测定方法的研究[J].郑州师范教育, 2013, 2(2):43-45.