聚乙二醇筛查法应用于高泌乳素血症诊断中的临床意义
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摘要
目的探究聚乙二醇筛查法在高泌乳血症诊断中的临床意义。方法选取260例HP患者作为观察组以及80例健康体检者作为对照组。应用雅培I2000全自动化学发光检测仪对两组标本进行PRL浓度测定,并将标本进行PEG筛查法沉淀后再次进行PRL浓度测定,将所得数据进行对比。结果观察组检出MP67例,检出率25.77%,对照组检出MP3例,检出率3.75%,二者差异有统计学意义(P<0.05);HP组、MP组和对照组之间PEG沉淀前PRL浓度差异有统计学意义(P<0.05),沉淀后HP组和MP组以及对照组差异有统计学意义(P<0.05),MP组和对照组差异无统计学意义(P>0.05);MP在HP引起的各种临床疾病中检出情况差异无统计学意义(P>0.05)。结论应用聚乙二醇筛查法对于排除MPRL对HP诊断的干扰具有可行性,对降低HP的误诊率有着重要的意义。
Objective To explore the clinical signification of polyethylene glycol screening method on the diagnosis of hyperprolactinemiat. Methods We selected 260 patients with hyperprolactinemia as observation group and 80 healthy individuals as control group. The two groups were measured with Abbott laboratories I2000 automatic chemiluminescence detector about the concentration of PRL. Then the groups were measured again after they were precipitated by the polyethylene glycol and the result data were analyzed. Results 78 cases of MP were checked out from the observation group and 3 cases were checked out from the control group. The proportion was 25. 77% and 3. 75% respectively,and the differences were statistically significant(P < 0. 05). The difference of the concentration of PRL before precipitated by PEG among the HP group,the MP group and the control group were statistically significant(P < 0. 05). After precipitating,the difference of the concentration of PRL between the HP group and the MP group were statistically significant(P < 0. 05),as well as the HP group and the control group. However,there was no difference between the MP group and the control group(P > 0. 05). The checking conditions of MP in some diseases caused by HP had no difference(P > 0. 05). Conclusion The use of polyethylene glycol screening method is a practicable method to eliminate the interference of MPRL on the diagnosis in HP. It has significance reducing misdiagnosis rate of HP.
Objective To explore the clinical signification of polyethylene glycol screening method on the diagnosis of hyperprolactinemiat. Methods We selected 260 patients with hyperprolactinemia as observation group and 80 healthy individuals as control group. The two groups were measured with Abbott laboratories I2000 automatic chemiluminescence detector about the concentration of PRL. Then the groups were measured again after they were precipitated by the polyethylene glycol and the result data were analyzed. Results 78 cases of MP were checked out from the observation group and 3 cases were checked out from the control group. The proportion was 25. 77% and 3. 75% respectively,and the differences were statistically significant(P < 0. 05). The difference of the concentration of PRL before precipitated by PEG among the HP group,the MP group and the control group were statistically significant(P < 0. 05). After precipitating,the difference of the concentration of PRL between the HP group and the MP group were statistically significant(P < 0. 05),as well as the HP group and the control group. However,there was no difference between the MP group and the control group(P > 0. 05). The checking conditions of MP in some diseases caused by HP had no difference(P > 0. 05). Conclusion The use of polyethylene glycol screening method is a practicable method to eliminate the interference of MPRL on the diagnosis in HP. It has significance reducing misdiagnosis rate of HP.
引文
[1]石青峰,杨峻,秦辛玲.高泌乳素血症患者在不同时段巨泌乳素水平的初步分析[J].国际检验医学杂志,2013,34(8):932-933.
[2]陈永健.筛检巨泌乳素血症的聚乙二醇沉淀法建立及其临床应用[D].杭州:浙江大学,2014:1.
[3]陈丽芬,朱惠君,黄雪飞,等.聚乙二醇沉淀法筛查巨泌乳素对高泌乳素血症的诊断价值[J].黑龙江医学,2016,40(9):875-876.
[4]GEORGE N KOUKOULIS.Macroprolactinemia:an unnoticeable factor[J].Hormones,2003,2(2):91-92.
[5]秦辛玲,石青峰,姚军.巨泌乳素筛查在高泌乳素血症诊断中的临床意义[J].检验医学与临床,2015,12(5):672-673.
[6]AMADORI P,DILBERIS C,MARCOLLA A,et al.Macroprolactinemia:predictability on clinical basis and detection by PEG precipitation with two different immunometric methods[J].J Endocrinol Invest,2003,26(2):148-156.
[7]SCHNEIDER W,MARCOWITZ S,AL-SHAMMARI S,et al.Reactivity of macroprolactin in common automated immunoassays[J].Clin Biochem,2001,34(6):469-473.
[8]王霞,刘金玲,高硕.聚乙二醇沉淀法与凝胶色谱层析法检测巨泌乳素的相关性研究[J].检验医学,2013,28(9):824-827.
[2]陈永健.筛检巨泌乳素血症的聚乙二醇沉淀法建立及其临床应用[D].杭州:浙江大学,2014:1.
[3]陈丽芬,朱惠君,黄雪飞,等.聚乙二醇沉淀法筛查巨泌乳素对高泌乳素血症的诊断价值[J].黑龙江医学,2016,40(9):875-876.
[4]GEORGE N KOUKOULIS.Macroprolactinemia:an unnoticeable factor[J].Hormones,2003,2(2):91-92.
[5]秦辛玲,石青峰,姚军.巨泌乳素筛查在高泌乳素血症诊断中的临床意义[J].检验医学与临床,2015,12(5):672-673.
[6]AMADORI P,DILBERIS C,MARCOLLA A,et al.Macroprolactinemia:predictability on clinical basis and detection by PEG precipitation with two different immunometric methods[J].J Endocrinol Invest,2003,26(2):148-156.
[7]SCHNEIDER W,MARCOWITZ S,AL-SHAMMARI S,et al.Reactivity of macroprolactin in common automated immunoassays[J].Clin Biochem,2001,34(6):469-473.
[8]王霞,刘金玲,高硕.聚乙二醇沉淀法与凝胶色谱层析法检测巨泌乳素的相关性研究[J].检验医学,2013,28(9):824-827.