棉花优异品质及抗性基因挖掘与分子育种
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摘要
棉花品质、产量、抗性等均是多基因控制的数量性状,其表现型受基因型和环境的共同影响,且纤维品质和产量性状之间存在较大的负相关,采用常规育种手段实现棉花纤维品质与产量的同步改良具难度很大,针对棉花品质、产量、抗性等性状形成的遗传基础这一关键科学问题,棉花品种分子设计研究团队以陆陆种内分离群体、陆海种间分离群体等材料,交叉利用分子数量遗传学、功能基因组学和分子育种学等研究手段,在陆地棉优质品质及抗性基因挖掘、海岛棉优质品质及抗性基因挖掘以及分子标记辅助方法开发及棉花新品种培育等方面取得了一系列研究成果。为棉花品质、产量、抗性性状形成的同步改良奠定了重要基础。
The fiber quality, yield and resistance of cotton were all the quantitative traits which were controlled by multi-genes and affected by the genotype and environment. As the fiber quality traits and yield traits showed negative correlation, improving the fiber quality and yield of cotton is difficult synchronously. Aiming at the key scientific problem that the hereditary basis of fiber quality, yield and resistance, the team of molecular design of cotton varieties used the population which were constructed by two Gossypium hirsutum of intraspecific and population constructed by one G. hirsutum and one G. barbadense of interspecific, combined the subject of molecular quantitative genetics, functional genomics and molecular breeding the genes related to high fiber quality and resistance in G. hirsutum, and G. barbadense were discovered, the method of molecular mark assisted breeding was developed and the new cotton varieties were cultivated. These results could provide new data and materials to solve the problem of the large scientific problem that the hereditary basis of fiber quality, yield and resistance.
The fiber quality, yield and resistance of cotton were all the quantitative traits which were controlled by multi-genes and affected by the genotype and environment. As the fiber quality traits and yield traits showed negative correlation, improving the fiber quality and yield of cotton is difficult synchronously. Aiming at the key scientific problem that the hereditary basis of fiber quality, yield and resistance, the team of molecular design of cotton varieties used the population which were constructed by two Gossypium hirsutum of intraspecific and population constructed by one G. hirsutum and one G. barbadense of interspecific, combined the subject of molecular quantitative genetics, functional genomics and molecular breeding the genes related to high fiber quality and resistance in G. hirsutum, and G. barbadense were discovered, the method of molecular mark assisted breeding was developed and the new cotton varieties were cultivated. These results could provide new data and materials to solve the problem of the large scientific problem that the hereditary basis of fiber quality, yield and resistance.
引文
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[2]张天真,郭旺珍.棉花分子育种的现状、问题与展望[J].中国农业科技导报,2007,9(2):19-25.Zhang Tianzhen,Guo Wangzhen.Molecular breeding in upland cotton:Present status,problem and prospect[J].Journal of Agricultural Science and Technology,2007,9(2):19-25.
[3]李付广,袁有禄.棉花分子育种进展与展望[J].中国农业科技导报,2011,13(5):1-8.Li Fuguang,Yuan Youlu.Progress and prospec of cotton molecular breeding[J].Journal of Agricultural Science and Technology,2011,13(5):1-8.
[4]Shappley Z W,Jenkins J N,Zhu J,et al.Quantitative trait locus associated with agronomic and fiber traits of upland cotton[J].J Cott Sci,1998,2(4):153-163.
[5]Ulloa M,Cantrell R G,Percy R,et al.QTL analysis of stomatal conductance and relationship to lint yield in an interspecific cotton[J].Journal of Cotton Science,2000(4):10-18.
[6]Sun Fuding,Zhang Jianhong,Zhang Jinfeng,et el.QTL mapping for fiber quality traits across multiple generations and environments in upland cotton[J].Molecular Breeding,2012,30(1):569-582.
[7]Jamshed M,Jia Fei,Gong Juwu,et al.Identification of stable quantitative trait loci(QTLs)for fiber quality traits across multiple environments in Gossypium hirsutum recombinant inbred line population[J].BMC Genomics,2016,17:197.
[8]Zhang Zhen,Li Junwen,Jamshed M,et al.High resolution consensus mapping of quantitative trait loci for fiber strength,length and micronaire on chromosome 25 of the upland cotton(Gossypium hirsutum L.)[J].PLo S One,2015,10(8):e0135430.
[9]Zhang Zhen,Shang Haihong,Shi Yuzhen,et al.Construction of a high-density genetic map by specific locus amplified fragment sequencing(SLAF-seq)and its application to quantitative trait loci(QTL)analysis for boll weight in upland cotton(Gossypium hirsutum)[J].BMC Plant Biol,2016,16(1):79.
[10]Zhang Zhen,Ge Qun,Liu Aiying,et al.Construction of a high-density genetic map and its application to QTL identification for fiber strength in upland cotton[J].Crop Science,2017,57(2):774-788.
[11]易成新,张天真,郭旺珍.陆地棉衣分QTL的形态和RAPD的分子标记筛选[J].作物学报,2001,6(27):781-786.Yi Chengxin,Zhang Tianzhen,Guo Wangzhen.Morphological and molecular tagging of lint percent QTLs in upland cotton[J].Acta Agronomica Sinica,2001,6(27):781-786.
[12]沈新莲.陆地棉纤维品质QTL的筛选、定位及其应用[D].南京:南京农业大学,2004.Shen Xinlian.Molecular tagging,location,and application of QTLs for fiber properties in upland cotton[D].Nanjing:Nanjing Agricultural University,2004.
[13]孔凡金,李俊文,龚举武,等.不同遗传背景下陆地棉衣分和子指性状QTL定位[J].中国农学通报,2011,27(18):104-109.Kong Fanjin,Li Junwen,Gong Juwu,et al.QTL mapping for lint percentage and seed index in upland cotton(Gossypium hirsutum L.)of different genetic backgrounds[J].Chinese Agricultural Science Bulletin,2011,27(18):104-109.
[14]叶祯维,邓晓英,石玉真,等.杂交棉中棉所70后代分离群体产量品质的表型变异分析[J].棉花学报,2016,28(1):1-10.Ye Zhenwei,Deng Xiaoying,Shi Yuzhen,et al.Analysis of phenotypic variation in fiber yield and quality in segregative populations of hybrid cotton CCRI 70[J].Cotton Science,2016,28(1):1-10.
[15]Shao Qianshun,Zhang Fengjiao,et al.Identifying QTL for fiber quality traits with three upland cotton(Gossypium hirsutum L.)populations[J].Euphytica,2014,198(1):43-58.
[16]Yang Xinlei,Zhou Xiaodong,et al.Mapping QTL for cotton fiber quality traits using simple sequence repeat markers,conserved intron-scanning primers,and transcript-derived fragments[J].Euphytica,2015,201(2):215-230.
[17]Yu Jiwen,Zhang Ke,Li Shuiyang,et al.Mapping quantitative trait loci for lint yield and fiber quality across environments in a Gossypium hirsutum×Gossypium barbadense backcross inbred line population[J].Theoretical&Applied Genetics,2013,126(1):275-287.
[18]马建江,吴嫚,裴文峰,等.棉花种子发育过程中脂肪及脂肪酸积累模式研究[J].棉花学报,2015,27(2):95-103.Ma Jianjiang,Wu Man,Pei Wenfeng,et al.The oil and fatty acid accumulation patterns in developing cottonseeds of Xuzhou142 and its fiberless and fuzeeless mutant[J].Cotton Science,2015,27(2):95-103.
[19]Wang Nuohan,Ma Jianjiang,Pei Wenfeng,et al.A genome-wide analysis of the lysophosphatidate acyltransferase(LPAAT)gene family in cotton:organization,expression,sequence variation,and association with seed oil content and fiber quality[J].BMC Genomics,2017,18(1):218.
[20]Palanga K K,Jamshed M,Rashid H O,et al.Quantitative trait locus mapping for verticillium wilt resistance in an upland cotton recombinant inbred line using SNP-based high density genetic map[J].Frontiers in plant science,2017,doi:10.3389/fpls.2017.00382.
[21]Percy R G,Cantrell R,Zhang Jinfa.Genetic Variation for agronomic and fiber properties in an introgressed recombinant inbred population of cotton[J].Crop Science,2006(3),46:1311-1317.
[22]石玉真.陆海棉花杂交F1代及其回交后代主要经济性状的遗传变异分析[D].北京:中国农业科学院,2009.Shi Yuzhen.Annlysis og genetic variation of main ecomic traits of interspecific hybrid between Gossypium hirsutum L.and G.baradense L.and its backcross populations[D].Beijing:Chinese Academy of Agricultural Sciences,2009.
[23]Zhang Jingfa,Wu Man,Yu Jiwen,et al.Breeding potential of introgression lines developed from interspecific crossing between upland cotton(Gossypium hirsutum)and Gossypium barbadense:Heterosis,combining ability and genetic effects[J].PLo S ONE,2016,11(1):e0143646.
[24]张宝才.AB-QTL法定位海岛棉优异纤维品质基因和抗黄萎病基因[D].北京:中国农业科学院,2006.Zhang Baocai.QTL Analysis of fiber qualitr and resistance to verticillium wilt using G.hirsutum×G.barbadense backcross populations[D].Beijing:Chinese Academy of Agricultural Sciences,2006.
[25]杨泽茂,李骏智,李爱国,等.利用高代回交和分子标记辅助选择构建棉花染色体片段代换系[J].分子植物育种,2009,7(2):233-241.Yang Zemao,Li Junzhi,Li Aiguo,et al.Developing chromosome segment substitution lines(CSSLs)in cotton(Gossypium)using advanced backcross and MAS[J].Molecular Plant Breeding,2009,7(2):233-241.
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