应用傅立叶变换近红外光谱检测婴幼儿配方奶粉中的乳糖含量
|
摘要
利用傅立叶变换近红外光谱(FT-NIR)技术结合偏最小二乘法(PLS)对婴幼儿配方奶粉中的乳糖进行快速检测分析。搜集94个不同产地、不同品牌的婴幼儿配方奶粉中乳糖的实验室数据,并采集婴幼儿配方奶粉的近红外光谱图,选择最优的光谱预处理方法,优化、验证和建立模型,并预测6个未知品牌的婴幼儿配方奶粉样品的乳糖含量。结果表明,不同阶段的婴幼儿配方奶粉有非常相似的近红外特征图谱,图谱处理后的方差为95.4235%,该模型测定的乳糖值与高效液相色谱(HPLC)法测定的乳糖值之间的平均相对误差均≤0.67%,相对标准偏差(RSD)均≤0.88%,均符合误差范围。该方法可以无损、快速、高效地测定婴幼儿配方奶粉中的乳糖含量。
Th e lactose content in infant formula milk powder was rapidly detected and analyzed by Fourier transform near-infrared spectroscopy(FT-NIR) combined with partial least squares(PLS). 94 samples of infant formula milk powder from different originals and brands were collected,and NIR spectra of all infant formula milk powder were collected. The optimal pre-treatment method of spectra was chosen, and then the model was optimized, verified, and established, and the lactate content of 6 samples of unknown infant formula milk powder were forecasted. The results showed that different infant formula milk powder had the same NIR spectrum, the variance of the spectra after treatment was 95.4235%, the average relative errors of lactose values determined by the model and HPLC method were less than 0.67%, the relative standard deviation(RSD) was less than 0.88%,and all of them were conformed to the error range. The results showed that method could determine lactate content in infant formula milk powder nondestructively, rapidly and efficiently.
Th e lactose content in infant formula milk powder was rapidly detected and analyzed by Fourier transform near-infrared spectroscopy(FT-NIR) combined with partial least squares(PLS). 94 samples of infant formula milk powder from different originals and brands were collected,and NIR spectra of all infant formula milk powder were collected. The optimal pre-treatment method of spectra was chosen, and then the model was optimized, verified, and established, and the lactate content of 6 samples of unknown infant formula milk powder were forecasted. The results showed that different infant formula milk powder had the same NIR spectrum, the variance of the spectra after treatment was 95.4235%, the average relative errors of lactose values determined by the model and HPLC method were less than 0.67%, the relative standard deviation(RSD) was less than 0.88%,and all of them were conformed to the error range. The results showed that method could determine lactate content in infant formula milk powder nondestructively, rapidly and efficiently.
引文
[1]郭锦杰.婴幼儿肠道微生物的发育及最接近母乳的配方奶粉研究进展[J].食品安全导刊,2017(12):47-49.
[2]唐玮,杨敏,于源,等.乳糖诱导大肠杆菌产褐藻胶裂解酶的条件优化[J].食品工业科技,2017(15):134-139.
[3]黄闽燕,钟小伶.莱因-埃农氏法测定婴幼儿奶粉中乳糖含量[J].中国卫生检验杂志,2005(12):126-130.
[4]刘左仪,陈海扬.奶粉乳糖含量用碘量法和廉-埃农氏两种方法测定的比较[J].中国乳品工业,1995,23(6):286-288.
[5]郭秀春,郭小白,张苗苗,等.莱茵-埃农氏法和高效液相色谱-示差折光法检测奶粉中乳糖和蔗糖的比较研究[J].食品科学,2016,37(12):139-143
[6]熊建飞,周光明,许丽,等.离子色谱法测定奶粉中的葡萄糖、蔗糖和乳糖[J].食品科学,2012,33(8):176-179.
[7]吴斌.酪蛋白和乳糖的营养价值及提取方法[J].世界最新医学,2015(23):256
[8]徐宗良,夏元军,巩军.近红外快速检测奶粉乳清粉成分研究[J].中国乳品工业,2002,30(2):40-41.
[9]杨莉,陶雁,吴建国,等.近红外反射光谱法测定浓缩乳清蛋白品质的新方法[J].中国乳品工业,2001,29(6):41-43.
[10]李长滨,党美珠,崔丽伟,等.近红外光谱技术在食品检测领域的应用[J].郑州牧业工程高等专科学校学报,2014,34(3):23-25.
[11]SANDOR B.The application of near infrared spectroscopy(NIR)technique for non-destructive investigation of mixed milk powder products[J].Mljekarstvo,2011,51(3):263-272.
[12]中华人民共和国卫生部.GB 5413.5-2010食品安全国家标准婴幼儿食品和乳品中乳糖、蔗糖的测定[S].北京:中国标准出版社,2010.
[13]张卫卫,刘建学,韩四海,等.白酒基酒中醛类物质的傅里叶变换近红外光谱检测[J].食品科学,2016,37(6):111-115.
[14]MAUER L J,CHEMYSHOVA A,HIATT A,et al.Melamine detection in infant formula powder using near-and mid-infrared spectroscopy[J].JAgr Food Chem,2009,57(10):3974-3980.
[15]张明锦,张世芝,杜一平.多模型共识偏最小二乘法用于近红外光谱定量分析[J].分析试验室,2012(4):102-105.
[16]褚小立,袁洪福,陆婉珍.近红外分析中光谱预处理及波长选择方法进展与应用[J].化学进展,2004,16(4):528-542.
[17]严衍禄,陈斌,朱大洲,等.近红外光谱分析基础及应用[M].北京:中国轻工业出版社,2005:125-126.
[18]ZHAO J,QIN Q,CHEN Q,et al.Simultaneous determination of amino acid nitrogen and total acid in soy sauce using near infrared spectroscopy combined with characteristic varlables selecton[J].Food Sci Technol Int,2013,19(4):305-314.
[19]杨智灵,李涛,任保增.近红外光谱技术在食品安全检测中的最新研究进展[J].食品与机械,2013,29(5):237-240.
[20]何佳艳,李亭郭,长凯近,等.近红外光谱法快速无损测定奶粉的脂肪含量[J].食品与发酵工业,2017,43(7):228-233.
[2]唐玮,杨敏,于源,等.乳糖诱导大肠杆菌产褐藻胶裂解酶的条件优化[J].食品工业科技,2017(15):134-139.
[3]黄闽燕,钟小伶.莱因-埃农氏法测定婴幼儿奶粉中乳糖含量[J].中国卫生检验杂志,2005(12):126-130.
[4]刘左仪,陈海扬.奶粉乳糖含量用碘量法和廉-埃农氏两种方法测定的比较[J].中国乳品工业,1995,23(6):286-288.
[5]郭秀春,郭小白,张苗苗,等.莱茵-埃农氏法和高效液相色谱-示差折光法检测奶粉中乳糖和蔗糖的比较研究[J].食品科学,2016,37(12):139-143
[6]熊建飞,周光明,许丽,等.离子色谱法测定奶粉中的葡萄糖、蔗糖和乳糖[J].食品科学,2012,33(8):176-179.
[7]吴斌.酪蛋白和乳糖的营养价值及提取方法[J].世界最新医学,2015(23):256
[8]徐宗良,夏元军,巩军.近红外快速检测奶粉乳清粉成分研究[J].中国乳品工业,2002,30(2):40-41.
[9]杨莉,陶雁,吴建国,等.近红外反射光谱法测定浓缩乳清蛋白品质的新方法[J].中国乳品工业,2001,29(6):41-43.
[10]李长滨,党美珠,崔丽伟,等.近红外光谱技术在食品检测领域的应用[J].郑州牧业工程高等专科学校学报,2014,34(3):23-25.
[11]SANDOR B.The application of near infrared spectroscopy(NIR)technique for non-destructive investigation of mixed milk powder products[J].Mljekarstvo,2011,51(3):263-272.
[12]中华人民共和国卫生部.GB 5413.5-2010食品安全国家标准婴幼儿食品和乳品中乳糖、蔗糖的测定[S].北京:中国标准出版社,2010.
[13]张卫卫,刘建学,韩四海,等.白酒基酒中醛类物质的傅里叶变换近红外光谱检测[J].食品科学,2016,37(6):111-115.
[14]MAUER L J,CHEMYSHOVA A,HIATT A,et al.Melamine detection in infant formula powder using near-and mid-infrared spectroscopy[J].JAgr Food Chem,2009,57(10):3974-3980.
[15]张明锦,张世芝,杜一平.多模型共识偏最小二乘法用于近红外光谱定量分析[J].分析试验室,2012(4):102-105.
[16]褚小立,袁洪福,陆婉珍.近红外分析中光谱预处理及波长选择方法进展与应用[J].化学进展,2004,16(4):528-542.
[17]严衍禄,陈斌,朱大洲,等.近红外光谱分析基础及应用[M].北京:中国轻工业出版社,2005:125-126.
[18]ZHAO J,QIN Q,CHEN Q,et al.Simultaneous determination of amino acid nitrogen and total acid in soy sauce using near infrared spectroscopy combined with characteristic varlables selecton[J].Food Sci Technol Int,2013,19(4):305-314.
[19]杨智灵,李涛,任保增.近红外光谱技术在食品安全检测中的最新研究进展[J].食品与机械,2013,29(5):237-240.
[20]何佳艳,李亭郭,长凯近,等.近红外光谱法快速无损测定奶粉的脂肪含量[J].食品与发酵工业,2017,43(7):228-233.