水稻特异性失绿突变株表型性状及遗传分析
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摘要
自然变异的叶色突变体lgs(t),在幼苗期叶片出现大部分或完全白化,分蘖期叶色逐渐转变为不规则绿白条纹,为了研究该性状发生原因,进一步挖掘其利用价值,通过对突变性状表现期的生理指标、农艺性进行调查状及遗传分析。结果表明,叶色失绿与叶绿素含量紧密相关,叶绿素a和叶绿素b含量均显著降低;白化现象的出现使得叶片中可溶性糖含量降低,游离氨基酸含量呈现增加的趋势,进而影响结实率和千粒重等产量性状;遗传分析表明该性状受一个单隐性核基因控制。
Lose green specific Mutants lgs(t) partially or completely whitened at the seedling stage was isolated from natural mutation. The leaf turns to irregular green and white stripes at tillering stage. For studying the mechanism of leaf color mutation and the prospects of utilizing. Physiological and agronomic characters were investigated and genetic analysis. The results showed that, loss of green was closely related to chlorophyll content, which appears significantly low. Albino phenomenon made the soluble sugar content decreased and increased the free amino acids, thereby affected the yield traits of seed-setting rate and 1000-grain weight. Genetic analysis showed that the trait was controlled by a single recessive nuclear gene.
Lose green specific Mutants lgs(t) partially or completely whitened at the seedling stage was isolated from natural mutation. The leaf turns to irregular green and white stripes at tillering stage. For studying the mechanism of leaf color mutation and the prospects of utilizing. Physiological and agronomic characters were investigated and genetic analysis. The results showed that, loss of green was closely related to chlorophyll content, which appears significantly low. Albino phenomenon made the soluble sugar content decreased and increased the free amino acids, thereby affected the yield traits of seed-setting rate and 1000-grain weight. Genetic analysis showed that the trait was controlled by a single recessive nuclear gene.
引文
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[15]Shao J R,Wang Y,Z H,et al.Ultrastructural observation on chloroplast of the t ermo-sensitive green-yellow banded leaf mutant in rice(Oryza sativa ssp.indica)[J].Journal of Integrative Plant Biology,1999,41(1):20-24.
[16]崔海瑞,夏英武,高明尉.温度对水稻突变体W1叶色及叶绿素生物合成的影响[J].核农学报,2001,15(5):269-273.
[17]张继林,孙元敏,郭绍铮.高产小麦营养生理特性与高效施肥技术研究[J].中国农业科学,1988,3(4):39-45.
[18]钱前,程式华.水稻遗传学和功能基因组学[M].北京:科学出版社,2006.
[2]谢戎,曾正明,刘成元,等.水稻间段失绿回复突变体特性分析[J].西南农业学报,2007,20(1):1-4.
[3]张毅,吕俊,李云峰,等.水稻白化转绿基因对农艺性状和外观米质的影响[J].作物学报,2008,34(2):284-289.
[4]孙焕明.水稻苍白叶突变体pgl3(t)的遗传分析和基因定位[D].扬州:扬州大学,2008.
[5]李娜,储黄伟,文铁桥,等.突变体的遗传分析与基因定位[J].上海农业学报,2007,23(1):1-4.
[6]胡景涛,张甲,李园园,等.水稻白色中脉Oswm2的遗传分析与分子标记定位[J].遗传,2008,30(9):1201-1206.
[7]张道允,巩子英.一个新的水稻斑马叶突变(zebra leaf1)对叶绿体发育的影响[J].分子细胞生物学报,2008,41(5):417-420.
[8]许凤华,程治军,王久林.水稻白条纹叶Gws基因的精细定位与遗传分析[J].作物学报,2010,36(5):713-720.
[9]张红林,程建峰,刘跃清,等.白化转斑叶籼型水稻不育系高光A的创制及特征特性[J].中国水稻科学,2010,24(6):587-594.
[10]杨洁.一个水稻白苗复绿性状的遗传特性和基因定位研究[D].武汉:华中农业大学,2008.
[11]李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000:195-203.
[12]周光来.丁草胺对水稻根系活力和C/N的影响[J].湖北民族学院学报:自然科学版,2002,20(2):37-339.
[13]邱义兰,李红,彭克勤,等.水稻“斑马叶”突变体B411叶绿体超微结构的观察[J].作物学报,2010,36(1):184-190.
[14]张力科,李志彬,刘海燕,等.两个新的水稻叶色突变体形态结构与遗传定位研究[J].中国农业科,2010,43(2):223-229.
[15]Shao J R,Wang Y,Z H,et al.Ultrastructural observation on chloroplast of the t ermo-sensitive green-yellow banded leaf mutant in rice(Oryza sativa ssp.indica)[J].Journal of Integrative Plant Biology,1999,41(1):20-24.
[16]崔海瑞,夏英武,高明尉.温度对水稻突变体W1叶色及叶绿素生物合成的影响[J].核农学报,2001,15(5):269-273.
[17]张继林,孙元敏,郭绍铮.高产小麦营养生理特性与高效施肥技术研究[J].中国农业科学,1988,3(4):39-45.
[18]钱前,程式华.水稻遗传学和功能基因组学[M].北京:科学出版社,2006.