乙醇对高脂肪饮食大鼠肝和肾功能的不良影响
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摘要
目的研究高脂肪饮食模式下,长期摄入乙醇对大鼠脂代谢、血糖水平、肝功能和肾功能的影响。方法雄性SD大鼠适应性喂养后,按体重随机分为阴性对照组12只,模型组48只,分别给予正常饲料与模型饲料,按照《辅助降血脂功能评价方法》喂养14 d建立混合型高脂血症动物模型,眼内眦取血测定血脂指标,根据血清总胆固醇(TC)水平将模型组分为模型对照组和低、中、高3个剂量组,每组12只动物。除空白对照组外,各组均给予高脂饲料,灌胃乙醇浓度分别为0%、11.3%、22.5%和45%,连续30 d后,测量各组体重,眼内眦取血测定各项生化指标。结果造模结果显示,与阴性对照组相比,模型组TC、HDL-C、LDL-C和TG均明显升高,差异有统计学意义(P<0.05)。给予乙醇30 d后,与模型对照组相比,高剂量组ALP、UREA明显降低,血糖(GLU)明显升高;中剂量组TG明显降低;中高剂量组CHE、LDH明显降低;低、中和高剂量组AST降低,差异有统计学意义(P<0.05)。结论在高脂肪饮食模式下,长期摄入乙醇能够影响高脂模型大鼠的脂肪代谢和糖代谢,并损伤肝功能和肾功能。
引文
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[3] Stickel F,Moreno C,Hampe J,et al.The genetics of alcohol dependence and alcohol-related liver disease[J].J Hepatol,2017,66(1):195-211.
[4] Heshmati E,Shirpoor A,Kheradmand F,et al.Chronic ethanol increases calcium/cal-modul independent protein kinaseIIδ gene expression and decreases monoamine oxidase am-ount in rat heart muscles:Rescue effect of Zingiber officinale(ginger)extract[J].Anato J Cardiol,2018,19(1):19-26.
[5] Tapia-Rojas C,Mira RG,Torres AK,et al.Alcohol consumption during adolescence:A link between mitochondrial damage and ethanol brain intoxication[J].Birth Defects Res,2017,109(20):1623-1639.
[6] Garaycoechea JI,Crossan GP,Langerin F,et al.Alcohol and endogenous aldehydes damage chromosomes and mutate stem cells[J].Nature,2018,553:171-177.
[7] 国家食品药品监督管理局.关于印发抗拉化功能评价方法等9个保健功能评价方法的通知[EB/OL].(2012-04-23).http://samr.ctda.gov.cn/WS01/CL0847/71257.html.
[8] Affane F,Louala S,El Imane Harrat N,et al.Hypolipidemic,antioxidant and antiatherogenic property of sardine by-products proteins in high-fat diet induced obese rats[J].Life Sci,2018,199:16-22.
[9] 严家荣,吴贤生,黎雄才,等.不同饲料配方复制混合型高脂身症大鼠模型的实验研究[J].实验动物科学,2018,35(5):11-16.
[10] 李晓军,马跃英,王海霞,等.降胆固醇益生菌联合大豆卵磷脂辅助降血脂保健食品的功能评价[J].中国微生态学杂志,2016,28(10):1126-1129.
[11] Du J,Shen L,Tan Z,et al.Betaine supplementation enhances lipid metabolism and improves insulin resistance in mice fed a high-fat diet[J].Nutrients,2018,10(2):131.
[12] 陈剑明,王丙信,伏爱国,等.高脂饮食诱导非酒精性脂肪肝大鼠模型的实验研究[J].中华中医药学刊,2016(11):2709-2712.
[13] 李灏,姜颖,贺福初.乙醇肝毒性及治疗的研究进展[J].中国药理学与毒理学杂志,2008,22(5):396-400.
[14] Ghosh DS,Warner JB,Warner DR,et al.Rodent models of alcoholic liver disease:role of binge ethanol administration[J].Biomolecules,2018,8(1):3-21.
[15] Chen SC,Tsai SP,Jhao JY,et al.Liver fat,hepatic enzymes,alkaline phosphatase and the risk of incident type 2 diabetes:a prospective study of 132,377 adults[J].Sci Rep,2017,7(1):4649-4658.
[16] Amin MA,El-Shahat ME,El-Garem N,et al.Assessment of serum level cholinesterase as a biomarker of liver cirrhosis in Egyptian cirrhotic patients[J].Gastroenterol Insights,2017,8(1):1-5.
[17] Leal S,Ricardo DJ,Joana B,et al.Heavy alcohol consumption effects on blood pressure and on kidney structure persist after long-term withdrawal[J].Kidney Blood Press Res,2017,42(4):664.
[18] 丁锐,卢姗,庞立园,等.不同浓度乙醇致小鼠急性酒精中毒与血糖关系的研究[J].中国微生态学杂志,2016,28(10):1130-1132.
[19] Fuchs CJ,Gonzalez JT,Beelen M,et al.Sucrose ingestion after exhaustive exercise accelerates liver,but not muscle glycogen repletion when compared to glucose ingestion in trained athletes[J].J Appl Physiol,2016,120(11):1328-1334.
[20] Amer SM,Bhopale KK,Kakumanu RD,et al.Hepatic alcohol dehydrogenase deficiency induces pancreatic injury in chronic ethanol feeding model of deer mice[J].Exp Mol Pathol,2018,104(1):89-97.