进水C/N对SNEDPR系统脱氮除磷的影响
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摘要
为了解同步硝化内源反硝化系统(SNEDPR)脱氮除磷性能,采用延时厌氧(180 min)/低氧(溶解氧0. 5～2. 0 mg·L~(-1))运行的SBR反应器,以人工配置的模拟废水为处理对象,先采用恒定进水C/N(为10),以实现SNEDPR的启动和聚磷菌(PAOs)的富集培养,再调控进水C/N值(分别为10、7. 5、5和2. 5),考察不同C/N对系统的脱氮除磷性能的影响.结果表明,当进水C/N为10,可实现SNEDPR的启动与深度脱氮除磷,出水PO3-4-P和总氮(TN)浓度分别平均为0. 1 mg·L~(-1)和8. 1mg·L~(-1),PO3-4-P去除率、TN去除率和SNED率平均值分别为99. 79%、89. 38%和58. 0%.当进水C/N由5提高至10时,系统维持良好的脱氮除磷性能,释磷量(PRA)和SNED率分别由16. 0 mg·L~(-1)和48. 0%提高至24. 4 mg·L~(-1)和69. 2%;当C/N为10时,TN和PO3-4-P去除率最高达94. 5%和100%;当C/N为2. 5时,系统失去脱氮、除磷性能,PRA和SNED率仅为1. 36 mg·L~(-1)和10%.在系统稳定运行阶段(C/N为10、7. 5和5),SNED率达85. 9%,出水NH_4~+-N、NO-x-N和PO3-4-P浓度平均为0、8. 1和0. 1 mg·L~(-1).
To determine the performance of nitrogen and phosphorus removal within a simultaneous nitrification endogenous denitrification system( SNEDPR),an extended anaerobic/low aerobic( dissolved oxygen: 0. 5-2. 0 mg·L~(-1))-operated sequencing batch reactor( SBR) was fed with simulation wastewater. The SBR was initiated under a constant influent C/N ratio of 10,with the simultaneous enrichment of polyphosphate-accumulating organisms( PAOs). It was then investigated at different influent C/N ratios of10,7. 5,5,and 2. 5. The experimental results indicated that,when the influent C/N ratio was 10,SNEDPR could be successfully started up. The effluent PO3-4-P and total nitrogen( TN) concentrations were 0. 1 mg·L~(-1) and 8. 1 mg·L~(-1). PO3-4-P efficiency,TN efficiency,and SNED efficiency were 99. 79%,89. 38%,and 58. 0%,respectively. When the influent C/N ratio increased from 5 to10,the nitrogen and phosphorus removal performance of the system improved with PRA,and SNED efficiency increased from 16. 0 m·L-1 and 48. 0% to 24. 4 mg·L~(-1) and 69. 2%,respectively. When the C/N ratio was 10,the TN and PO3-4-P removal efficiencies increased to 94. 5% and 100%,respectfully. When the C/N ratio was decreased to 2. 5,the nitrogen and phosphorus removal performance of the system decreased. The PRA and SNED efficiencies were only 1. 36 mg·L~(-1) and 10%,respectively. During the stable phase of the system( C/N ratio were 10,7. 5 and 5),SNED efficiency reached to 85. 9%,with the average effluent concentration of NH_4~+-N,NOx--N,and PO3-4-P being 0. 0,8. 1,and 0. 1 mg·L~(-1),respectively.
To determine the performance of nitrogen and phosphorus removal within a simultaneous nitrification endogenous denitrification system( SNEDPR),an extended anaerobic/low aerobic( dissolved oxygen: 0. 5-2. 0 mg·L~(-1))-operated sequencing batch reactor( SBR) was fed with simulation wastewater. The SBR was initiated under a constant influent C/N ratio of 10,with the simultaneous enrichment of polyphosphate-accumulating organisms( PAOs). It was then investigated at different influent C/N ratios of10,7. 5,5,and 2. 5. The experimental results indicated that,when the influent C/N ratio was 10,SNEDPR could be successfully started up. The effluent PO3-4-P and total nitrogen( TN) concentrations were 0. 1 mg·L~(-1) and 8. 1 mg·L~(-1). PO3-4-P efficiency,TN efficiency,and SNED efficiency were 99. 79%,89. 38%,and 58. 0%,respectively. When the influent C/N ratio increased from 5 to10,the nitrogen and phosphorus removal performance of the system improved with PRA,and SNED efficiency increased from 16. 0 m·L-1 and 48. 0% to 24. 4 mg·L~(-1) and 69. 2%,respectively. When the C/N ratio was 10,the TN and PO3-4-P removal efficiencies increased to 94. 5% and 100%,respectfully. When the C/N ratio was decreased to 2. 5,the nitrogen and phosphorus removal performance of the system decreased. The PRA and SNED efficiencies were only 1. 36 mg·L~(-1) and 10%,respectively. During the stable phase of the system( C/N ratio were 10,7. 5 and 5),SNED efficiency reached to 85. 9%,with the average effluent concentration of NH_4~+-N,NOx--N,and PO3-4-P being 0. 0,8. 1,and 0. 1 mg·L~(-1),respectively.
引文
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[17] Figdore B A,Stensel H D,Winkler M K H. Bioaugmentation of sidestream nitrifying-denitrifying phosphorus-accumulating granules in a low-SRT activated sludge system at low temperature[J]. Water Research,2018,135:241-250.
[18]方茜,张朝升,张可方,等.污泥龄及p H值对同步硝化反硝化过程的影响[J].广州大学学报(自然科学版),2008,7(3):50-54.
[19] Kong Y H,Beer M,Rees G N,et al. Functional analysis of microbial communities in aerobic-anaerobic sequencing batch reactors fed with different phosphorus/carbon(P/C)ratios[J].Microbiology,2002,148(8):2299-2307.
[20] Lo I W,Lo K V,Mavinic D S,et al. Contributions of biofilm and suspended sludge to nitrogen transformation and nitrous oxide emission in hybrid sequencing batch system[J]. Journal of Environmental Sciences,2010,22(7):953-960.
[21] Wang X X,Wang S Y,Xue T L,et al. Treating low carbon/nitrogen(C/N)wastewater in simultaneous nitrificationendogenous denitrification and phosphorous removal(SNDPR)systems by strengthening anaerobic intracellular carbon storage[J]. Water Research,2015,77:191-200.
[22]国家环境保护总局.水和废水监测分析方法[M].(第四版).北京:中国环境科学出版社,2002. 252-354.
[23]何理,高大文.基于反硝化聚磷菌的颗粒污泥的培养[J].中国环境科学,2014,34(2):383-389.He L, Gao D W. Cultivate of granular sludge based on denitrifying phosphate accumulating bacteria[J]. China Environmental Science,2014,34(2):383-389.
[24]郑兴灿,李亚新.污水除磷脱氮技术[M].北京:中国建筑工业出版社,1998. 36-62.
[25]张可方,张朝升,罗思音,等. SBR短程同步硝化反硝化耦合除磷的研究[J].中国给水排水,2010,26(7):65-67,70.Zhang K F,Zhang C S,Luo S Y,et al. Coupling of short-cut simultaneous nitrification and denitrification with denitrifying phosphorus removal in SBR[J]. China Water&Wastewater,2010,26(7):65-67,70.
[26] Smolders G J F,van der Meij J,van Loosdrecht M C M,et al.Model of the anaerobic metabolism of the biological phosphorus removal process:stoichiometry and pH influence[J].Biotechnology and Bioengineering,1994,43(6):461-470.
[27] Kerrn-Jespersen J P,Henze M. Biological phosphorus uptake under anoxic and aerobic conditions[J]. Water Research,1993,27(4):617-624.
[28] Moya J,Huili1ir C,Peredo K,et al. Modeling of simultaneous denitrification-anaerobic digestion-organic matter aerobic oxidation and nitrification in an anoxic-anaerobic-aerobic compact filter reactor[J]. Journal of Biotechnology,2012,160(3-4):176-188.
[2] Her J J,Huang J S. Influences of carbon source and C/N ratio on nitrate/nitrite denitrification and carbon breakthrough[J].Bioresource Technology,1995,54(1):45-51.
[3]王晓霞.低C/N比污水同步硝化反硝化除磷工艺与优化控制[D].北京:北京工业大学,2016.
[4] Zeng R J, Lemaire R, Yuan Z G, et al. Simultaneous nitrification,denitrification,and phosphorus removal in a labscale sequencing batch reactor[J]. Biotechnology and Bioengineering,2003,84(2):170-178.
[5]方茜,张朝升,张立秋,等.同时硝化/反硝化除磷工艺稳定性控制研究[J].给水排水,2014,40(11):132-135.
[6]潘芳,郭刚,王鸿,等.缺氧反应时间对反硝化除磷系统脱氮除磷效果的影响[J].四川环境,2014,33(1):36-40.Pan F,Guo G,Wang H,et al. Impact of anoxic reaction time on denitrifying phosphorus removal performance[J]. Sichuan Environment,2014,33(1):36-40.
[7] Gao D W,Yuan X J,Liang H. Reactivation performance of aerobic granules under different storage strategies[J]. Water Research,2012,46(10):3315-3322.
[8] Pollice A,Tandoi V,Lestingi C. Influence of aeration and sludge retention time on ammonium oxidation to nitrite and nitrate[J]. Water Research,2002,36(10):2541-2546.
[9]许松瑜.双泥折流板反硝化除磷工艺硝化菌的FISH检测研究[D].苏州:苏州科技学院,2011.Xu S Y. Investigation on the FISH detection of the nitrifying bacteria in two-sludge baffled reactor[D]. Suzhou:Suzhou University of Science and Technology,2011.
[10]闫骏,王淑莹,高守有,等.低溶氧下低C/N值生活污水的同步硝化反硝化[J].中国给水排水,2007,23(3):44-48.Yan J,Wang S Y,Gao S Y,et al. Simultaneous nitrification and denitrification of wastewater with low C/N ratio under low DO[J]. China Water&Wastewater,2007,23(3):44-48.
[11] Tetreault M J,Benedict A H,Kaempfer C,et al. Biological phosphorus removal:a technology evaluation[J]. Journal,1986,58(8):823-837.
[12]张万友,张兰河,杨涛,等.不同有机碳源对SBR工艺同步硝化反硝化影响[J].化工进展,2010,29(12):2395-2399.Zhang W Y,Zhang L H,Yang T,et al. Effects of organic carbon on simultaneous nitrification and denitrification in sequencing batch reactor[J]. Chemical Industry and Engineering Progress,2010,29(12):2395-2399.
[13]林金銮,张可方,方茜,等.碳源浓度对同步硝化反硝化协同除磷影响研究[J].环境科学与技术,2009,32(9):5-8.Lin J L, Zhang K F, Fang Q, et al. Effect of carbon concentration on simultaneous nitrification,de nitrification and phosphorus removal in SBR[J]. Environmental Science&Technology,2009,32(9):5-8.
[14]戴娴,王晓霞,彭永臻,等.进水C/N对富集聚磷菌的SNDPR系统脱氮除磷的影响[J].中国环境科学,2015,35(9):2636-2643.Dai X,Wang X X,Peng Y Z,et al. Effect of influent C/N ratio on simultaneous nitrification-denitrification and phosphorus removal(SNDPR)enriched with phosphorus accumulating organisms(PAOs)[J]. China Environmental Science,2015,35(9):2636-2643.
[15]林金銮,张可方,方茜,等. DO对同步硝化反硝化协同除磷的影响[J].化工环保,2009,29(2):109-112.Lin J L, Zhang K F, Fang Q, et al. Effect of DO on simultaneous nitrification-denitrification with phosphorus removal[J]. Environmental Protection of Chemical Industry,2009,29(2):109-112.
[16]戴娴,彭永臻,王晓霞,等.不同厌氧时间对富集聚磷菌的SNDPR系统处理性能的影响[J].中国环境科学,2016,36(1):92-99.Dai X,Peng Y Z,Wang X X,et al. Effect of different anaerobic time on the nutrient removal in simultaneous nitrificationdenitrification and phosphorus removal(SNDPR)systems enriched with phosphorus accumulating organisms[J]. China Environmental Science,2016,36(1):92-99.
[17] Figdore B A,Stensel H D,Winkler M K H. Bioaugmentation of sidestream nitrifying-denitrifying phosphorus-accumulating granules in a low-SRT activated sludge system at low temperature[J]. Water Research,2018,135:241-250.
[18]方茜,张朝升,张可方,等.污泥龄及p H值对同步硝化反硝化过程的影响[J].广州大学学报(自然科学版),2008,7(3):50-54.
[19] Kong Y H,Beer M,Rees G N,et al. Functional analysis of microbial communities in aerobic-anaerobic sequencing batch reactors fed with different phosphorus/carbon(P/C)ratios[J].Microbiology,2002,148(8):2299-2307.
[20] Lo I W,Lo K V,Mavinic D S,et al. Contributions of biofilm and suspended sludge to nitrogen transformation and nitrous oxide emission in hybrid sequencing batch system[J]. Journal of Environmental Sciences,2010,22(7):953-960.
[21] Wang X X,Wang S Y,Xue T L,et al. Treating low carbon/nitrogen(C/N)wastewater in simultaneous nitrificationendogenous denitrification and phosphorous removal(SNDPR)systems by strengthening anaerobic intracellular carbon storage[J]. Water Research,2015,77:191-200.
[22]国家环境保护总局.水和废水监测分析方法[M].(第四版).北京:中国环境科学出版社,2002. 252-354.
[23]何理,高大文.基于反硝化聚磷菌的颗粒污泥的培养[J].中国环境科学,2014,34(2):383-389.He L, Gao D W. Cultivate of granular sludge based on denitrifying phosphate accumulating bacteria[J]. China Environmental Science,2014,34(2):383-389.
[24]郑兴灿,李亚新.污水除磷脱氮技术[M].北京:中国建筑工业出版社,1998. 36-62.
[25]张可方,张朝升,罗思音,等. SBR短程同步硝化反硝化耦合除磷的研究[J].中国给水排水,2010,26(7):65-67,70.Zhang K F,Zhang C S,Luo S Y,et al. Coupling of short-cut simultaneous nitrification and denitrification with denitrifying phosphorus removal in SBR[J]. China Water&Wastewater,2010,26(7):65-67,70.
[26] Smolders G J F,van der Meij J,van Loosdrecht M C M,et al.Model of the anaerobic metabolism of the biological phosphorus removal process:stoichiometry and pH influence[J].Biotechnology and Bioengineering,1994,43(6):461-470.
[27] Kerrn-Jespersen J P,Henze M. Biological phosphorus uptake under anoxic and aerobic conditions[J]. Water Research,1993,27(4):617-624.
[28] Moya J,Huili1ir C,Peredo K,et al. Modeling of simultaneous denitrification-anaerobic digestion-organic matter aerobic oxidation and nitrification in an anoxic-anaerobic-aerobic compact filter reactor[J]. Journal of Biotechnology,2012,160(3-4):176-188.