皂角刺中总黄酮含量测定及其抗氧化活性研究
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摘要
目的测定回流法和超声法得到皂角刺醇提物和水提物中总黄酮含量,比较其体外抗氧化活性。方法采用紫外-可见分光光度法测定皂角刺提取物中总黄酮含量,FRAP法研究总黄酮体外抗氧化能力。结果皂角刺超声醇提物中总黄酮含量最高(1. 38%),4种提取物对Fe3+-TPTZ自由基离子均有一定清除作用,其中回流水提物清除作用最强。结论建立的方法简便、准确,可用于皂角刺中总黄酮的含量测定;回流水提物的抗氧化能力较强。
Objective To determine the content of total flavonoids in alcohol extracts and water extracts of Gleditsia sinensis by reflux method and ultrasound method,and to compare their antioxidant activities in vitro. Methods UV-visible spectrophotometry was used to determine the content of total flavonoids in the extracts of Gleditsia sinensis. FRAP method was used to study the antioxidant activity of total flavonoids in vitro. Results The content of total flavonoids in the ultrasonic ethanol extract of Gleditsia sinensis was the highest( 1. 38%). The four extracts had certain scavenging effect on free radical ions of Fe3 +-TPTZ, and the reflux water extract had the strongest scavenging effect. Conclusion The established method is simple and accurate,which can be used for the content determination of total flavonoids from Gleditsia sinensis. The reflux water extract has strongest antioxidant activity.
Objective To determine the content of total flavonoids in alcohol extracts and water extracts of Gleditsia sinensis by reflux method and ultrasound method,and to compare their antioxidant activities in vitro. Methods UV-visible spectrophotometry was used to determine the content of total flavonoids in the extracts of Gleditsia sinensis. FRAP method was used to study the antioxidant activity of total flavonoids in vitro. Results The content of total flavonoids in the ultrasonic ethanol extract of Gleditsia sinensis was the highest( 1. 38%). The four extracts had certain scavenging effect on free radical ions of Fe3 +-TPTZ, and the reflux water extract had the strongest scavenging effect. Conclusion The established method is simple and accurate,which can be used for the content determination of total flavonoids from Gleditsia sinensis. The reflux water extract has strongest antioxidant activity.
引文
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[3]刘艳明,徐青,薛兴亚,等.中药皂角刺的化学组成及液相色谱研究[J].精细化工,2006(10):967-971.
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[5]何峰,何光志,张利新,等.皂角刺含药血清诱导直肠癌细胞SW-480凋亡及其对Bcl-2/Bax mRNA表达的影响[J].中国实验方剂学杂志,2013,19(19):217-221.
[6]李岗,仙云霞,王晓,等.皂角刺化学成分及其抗肿瘤活性研究[J].中草药,2015,46(19):2846-2850.
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[8]吴丽红,任丽英,陈志钢.紫叶李果实总黄酮抗氧化性能实验研究[J].中国药业,2017,26(5):15-19.
[9]邓六勤,黎梅桂,吴宝仪.黄芪多糖的氧化降解及其抗氧化和抗肿瘤活性研究[J].中国药业,2018,27(1):13-16.
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[11]李菁,江坤,殷果,等.中药皂角刺的HPLC指纹图谱[J].沈阳药科大学学报,2017,34(8):660-666.
[12]赵梅,周淑琴.黄酮类化合物抗肿瘤作用及其相关机制研究进展[J].中国药业,2011,20(23):1-3.
[13]吴荣昆,黄小流,刘美金,等.井冈山马齿苋多糖的体外抗氧化作用及总糖含量测定[J].井冈山大学学报(自然科学版),2016,37(2):102-106.
[14]杨晓峪,李振麟,濮社班,等.皂角刺化学成分及药理作用研究进展[J].中国野生植物资源,2015,34(3):38-41.
[15]汪涛,承伟,王佩琪,等.皂角刺药学与临床应用的研究进展[J].锦州医学院学报,2005,26(6):60-62.