青蒿琥酯与奎宁治疗巴贝虫病的疗效观察
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摘要
目的比较青蒿琥酯和奎宁治疗巴贝虫病的疗效。方法取感染田鼠巴贝虫的Nod-Scid种鼠血,经腹腔注射接种BALB/c小鼠。自接种后第4d开始,分别经口灌胃相同剂量的青蒿琥酯和奎宁。采用姬姆萨染色检查小鼠红细胞田鼠巴贝虫感染率,并检测小鼠的体温、体重、食量以及各项生理指标。结果在接种后第9d,各组小鼠红细胞田鼠巴贝虫感染率均达到高峰,但青蒿琥酯治疗组小鼠的红细胞染虫率(25.5%)低于奎宁治疗组(43.0%)和对照组(45.5%);在接种后第18d各组小鼠红细胞染虫率均达到最低,青蒿琥酯治疗组小鼠的红细胞染虫率(2.5%)低于奎宁治疗组(6.0%)和对照组(6.5%),但不能清除红细胞内寄生的田鼠巴贝虫。结论青蒿琥酯抗巴贝虫效果优于奎宁,可以作为治疗巴贝虫病的候选药物。
Objective To compare artesunate and quinine as treatments for babesiosis. Methods BALB/c mice were intraperitoneally inoculated with 100μl of blood infected with Babesia microti,which was collected,heparinized,and diluted with sterile saline to achieve a 30%rate of RBC infection.After four days,the mice were treated with the same dose of artesunate or quinine via gavage;and mice in the control group received an equivalent volume of PBS.The percentage of RBCs infected with parasites and various physiological indices such as body temperature,weight,and appetite were determined for each mouse each day. Results On day 9after inoculation,the percentage of RBCs infected with parasites peaked in all of the mice,but the percentage of RBCs infected with parasites in mice treated with artesunate(25.5%)was lower than that in mice treated with quinine(43.0%)or controls(45.5%).On day 18 after inoculation,the percentage of RBCs infected with parasites in all of the mice reached its nadir.The percentage of RBCs infected with parasites in mice treated with artesunate(2.5%)was significantly lower than that in mice treated with quinine(6.0%)or controls(6.5%).However,artesunate did not eliminate B.microti infecting RBCs. Conclusion Artesunate is more effective as a treatment for babesiosis than quinine,and artesunate may be a potential treatment for babesiosis.
Objective To compare artesunate and quinine as treatments for babesiosis. Methods BALB/c mice were intraperitoneally inoculated with 100μl of blood infected with Babesia microti,which was collected,heparinized,and diluted with sterile saline to achieve a 30%rate of RBC infection.After four days,the mice were treated with the same dose of artesunate or quinine via gavage;and mice in the control group received an equivalent volume of PBS.The percentage of RBCs infected with parasites and various physiological indices such as body temperature,weight,and appetite were determined for each mouse each day. Results On day 9after inoculation,the percentage of RBCs infected with parasites peaked in all of the mice,but the percentage of RBCs infected with parasites in mice treated with artesunate(25.5%)was lower than that in mice treated with quinine(43.0%)or controls(45.5%).On day 18 after inoculation,the percentage of RBCs infected with parasites in all of the mice reached its nadir.The percentage of RBCs infected with parasites in mice treated with artesunate(2.5%)was significantly lower than that in mice treated with quinine(6.0%)or controls(6.5%).However,artesunate did not eliminate B.microti infecting RBCs. Conclusion Artesunate is more effective as a treatment for babesiosis than quinine,and artesunate may be a potential treatment for babesiosis.
引文
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[14]刘瑞爱,邓敏茹,Antonio S,等.青蒿琥酯治疗妊娠期重症疟疾患者的疗效观察[J].河北医学,2010,16(7):849-51.
[15]李世荣,王红,齐文杰.人巴贝西虫病研究进展[J].临床和实验医学杂志,2015,14(23):2014-7.
[16]薛武进,李凌云,陈佳琦.人感染田鼠巴贝斯虫的实验室诊断一例[J].检验医学,2014,29(5):553-5.
[2]魏金龙,周金林.巴贝斯虫感染对宿主红细胞及免疫系统的影响[J].中国动物传染病学报,2015,23(1):71-7.
[3]吴家俊,周金林.巴贝斯虫病研究进展[J].动物医学进展,2013,34(12):173-8.
[4]Wormser GP,Prasad A,Neuhaus E,et al.Emergence of resistance to azithromycin-atovaquone in immunocompromised patients with Babesia microti infection[J].Clin Infect Dis,2010,50(3):381-6.
[5]刘焕,邓胜林.青蒿琥酯与奎宁马斯静脉滴注治疗塞拉利昂儿童无并发症恶性疟疾的疗效比较[J].医学临床研究,2011,28(1):47-9.
[6]刘皋林.新编治疗药物学[M].北京:人民卫生出版社,2007:206.
[7]王婧,周绪正,李冰,等.抗巴贝斯虫药物的研究进展[J].中国畜牧兽医,2013,40(4):206-10.
[8]Rosalynn LO,Cheryl AL.Human babesiosis:pathogens,prevalence,diagnosis and treatment[J].Curr Clin Micro Rpt,2015,2(4):173-81.
[9]陈海亮,刘毅,邹中元,等.青蒿琥酯与奎宁治疗疟疾的疗效比较[J].江西医药,2014,49(1):57-9.
[10]Goo YK,Terkawi MA,Jia H,et al.Artesunate,apotential drug for treatment of Babesia infection[J].Parasito Int,2010,59(3):481-6.
[11]卢艳,蔡玉春,陈韶红.田鼠巴贝虫实验动物模型的建立[J].中国寄生虫学与寄生虫病杂志,2013,30(6):423-7.
[12]蒋明,张仪.人巴贝虫病研究进展[J].国际医学寄生虫病杂志,2014,41(2):94-104.
[13]张丽.青蒿琥酯治疗小儿恶性脑型疟疾43例疗效观察[J].宁夏医学杂志,2011,33(10):1033-4.
[14]刘瑞爱,邓敏茹,Antonio S,等.青蒿琥酯治疗妊娠期重症疟疾患者的疗效观察[J].河北医学,2010,16(7):849-51.
[15]李世荣,王红,齐文杰.人巴贝西虫病研究进展[J].临床和实验医学杂志,2015,14(23):2014-7.
[16]薛武进,李凌云,陈佳琦.人感染田鼠巴贝斯虫的实验室诊断一例[J].检验医学,2014,29(5):553-5.