光谱法研究G-四链体与盐酸巴马汀的相互作用
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摘要
采用光谱法探究了盐酸巴马汀与分子内G-四链体的相互作用。结果表明,盐酸巴马汀能够诱导富GDNA序列形成分子内G-四链体结构,并与其产生较强结合作用。随着G-四链体DNA的加入,盐酸巴马汀在波长535nm处的荧光强度明显增强,发射峰蓝移;紫外光谱表现出减色和红移现象,说明盐酸巴马汀可能堆积到G-四链体的尾部,或者插入到两个G-四分体之间形成夹心结构。等摩尔连续变化法表明两者的结合比为1∶1,运用Scatchard方程计算出二者的结合常数K为4.83×10~6 L/mol、结合位点n为0.75。圆二色滴定曲线和~1H NMR进一步验证两者之间存在相互作用。盐酸巴马汀对G-四链体/Hemin配合物的过氧化酶抑制实验证实作用模式为末端堆积。
The interaction of palmatine chloride and G-quadruplex was studied by spectroscopy.With the addition of G-quadruplex,the fluorescence intensity of palmatine appeared significant increase at 535 nm,and the peak blue shifted.The UV spectra showed the phenomena of hypochromic effect and red shift,which indicated palmatine might stake on the tail of G-quadruplex or insert into two G-quartets.The binding ratio was obtained as 1∶1 by equimolar continuous change method,and the binding constant was 4.83×10~6 L/mol and the binding site was 0.75 by the Scatchard equation.Nuclear magnetic resonance and circular dichroism spectra also proved the interaction of palmatine with the G-quadruplex.Inhibition of peroxidase activity of G-quadruplex/palmatine complex showed that the interaction mode between palmatine and G-quadruplex was terminal stacking.These data indicated that palmatine had strong binding ability with G-quadruplex DNA.
The interaction of palmatine chloride and G-quadruplex was studied by spectroscopy.With the addition of G-quadruplex,the fluorescence intensity of palmatine appeared significant increase at 535 nm,and the peak blue shifted.The UV spectra showed the phenomena of hypochromic effect and red shift,which indicated palmatine might stake on the tail of G-quadruplex or insert into two G-quartets.The binding ratio was obtained as 1∶1 by equimolar continuous change method,and the binding constant was 4.83×10~6 L/mol and the binding site was 0.75 by the Scatchard equation.Nuclear magnetic resonance and circular dichroism spectra also proved the interaction of palmatine with the G-quadruplex.Inhibition of peroxidase activity of G-quadruplex/palmatine complex showed that the interaction mode between palmatine and G-quadruplex was terminal stacking.These data indicated that palmatine had strong binding ability with G-quadruplex DNA.
引文
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