金花小檗提取物及组分对小鼠学习记忆能力及抗氧化能力的影响
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摘要
目的测定金花小檗根、茎和叶及根、茎提取物的总生物碱及其组分含量,研究根、茎提取物及组分对小鼠学习记忆能力和抗氧化能力的改善作用。方法采用酸性染料比色法测定金花小檗根、茎、叶和根、茎提取物及组分的总生物碱含量,以HPLC法同时测定药根碱、巴马汀和小檗碱的含量。用东莨菪碱制备记忆障碍小鼠模型,ig不同剂量的金花小檗根、茎提取物及组分30 d,以跳台实验和水迷宫实验检测根、茎提取物及组分对小鼠学习记忆的影响,测定血清丙二醛(MDA)和总超氧化物歧化酶(T-SOD)。结果金花小檗提取物及水溶性组分显著延长小鼠跳台实验的潜伏期(P<0.01),显著减少错误次数(P<0.01);在一定程度上,缩短水迷宫的定位航行时间(P<0.01),并显著减少错误次数(P<0.05)。根、茎提取物和水溶性组分能显著提高小鼠体内T-SOD活性(P<0.05、0.01),而显著降低MDA水平(P<0.05、0.01)。结论金花小檗根、茎提取物及水溶性组分对小鼠的学习记忆能力有一定的改善作用,对其体内抗氧化能力也有提高。
Objective To determine the content of total alkaloids and their components in roots,stems,and leaves of Berberis wilsonae,and investigate the effects of their extracts and components on the improvement of learning and memory ability and anti-oxidant activity of mice.Methods In this study,the total alkaloid content of roots,stems,leaves,and roots,stem extracts and components of B.wilsonae was determined by acid dye colorimetry.The HPLC method and conditions were established for determination jateorhizine,palmatine,and berberine.The memory impairment mice model was established with scopolamine,and the root and stem extracts and components of B.wilsonae were ig administrated for 30 d.Jumping platform test and water maze test were used to detect the effect of roots and stem extracts and components on learning and memory,and the levels of MDA and T-SOD in serum was determinated by kit method.Results The results showed that the latency of the mice in step-down test was significantly prolonged(P < 0.01),and the reduction of the number of errors was also significant(P < 0.01).To a certain extent,the place navigation time in water maze was shortened(P < 0.01),and the number of errors was reduced significantly(P < 0.05).Extracts and components can significantly increase the level of T-SOD(P < 0.05,0.01) in mice,and reduce the level of MDA significantly(P < 0.05,0.01).Conclusion The extracts and components of B.wilsonae had a certain effect on the improvement of learning and memory ability of mice,and the anti-oxidant capacity in vivo was also improved.
Objective To determine the content of total alkaloids and their components in roots,stems,and leaves of Berberis wilsonae,and investigate the effects of their extracts and components on the improvement of learning and memory ability and anti-oxidant activity of mice.Methods In this study,the total alkaloid content of roots,stems,leaves,and roots,stem extracts and components of B.wilsonae was determined by acid dye colorimetry.The HPLC method and conditions were established for determination jateorhizine,palmatine,and berberine.The memory impairment mice model was established with scopolamine,and the root and stem extracts and components of B.wilsonae were ig administrated for 30 d.Jumping platform test and water maze test were used to detect the effect of roots and stem extracts and components on learning and memory,and the levels of MDA and T-SOD in serum was determinated by kit method.Results The results showed that the latency of the mice in step-down test was significantly prolonged(P < 0.01),and the reduction of the number of errors was also significant(P < 0.01).To a certain extent,the place navigation time in water maze was shortened(P < 0.01),and the number of errors was reduced significantly(P < 0.05).Extracts and components can significantly increase the level of T-SOD(P < 0.05,0.01) in mice,and reduce the level of MDA significantly(P < 0.05,0.01).Conclusion The extracts and components of B.wilsonae had a certain effect on the improvement of learning and memory ability of mice,and the anti-oxidant capacity in vivo was also improved.
引文
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[19]李路扬,龙娓芳,万定荣,等.5种小檗属药用植物总生物碱及小檗碱的含量测定[J].中国实验方剂学杂志,2014,20(17):91-94.
[20]李晶.鱼油制剂改善小鼠记忆作用的实验研究[J].食品科学,2004,25(10):301-304.
[21]司茹,郑梦思,邹莉波.美藤果油辅助改善小鼠记忆的功效[J].食品科学,2017,38(9):202-206.
[22]吕姗珊,唐桂英,杨超,等.小儿黄龙颗粒对记忆障碍模型小鼠学习记忆功能的影响[J].中国实验方剂学杂志,2014,20(14):172-177.
[23]明建,曾凯芳,吴素蕊,等.天麻多糖PGEB-3-H对东莨菪碱所致小鼠学习记忆障碍的影响[J].食品科学,2010,31(3):246-249.
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[25]杨伟丽,马志刚,祁梅.甘肃不同产地三颗针不同部位生物碱的含量测定[J].甘肃医药,2009,28(5):349-351.
[26]何丹丹,张磊,刘力,等.骆驼蓬子总生物碱改善小鼠学习记忆能力[J].中成药,2015,37(3):478-482.
[27]王海波,王波,邸学,等.钩藤生物碱部位对AD大鼠学习记忆作用研究[J].时珍国医国药,2017,28(5):1027-1029.
[28]姜琳珊,李菲,聂晶,等.金钗石斛总生物碱对APP/PS1转基因小鼠学习记忆能力的影响[J].遵义医学院学报,2016,39(3):246-249.
[29]An L,Han X,Li H,et al.Effects and mechanism of cerebroprotein hydrolysate on learning and memory ability in mice[J].Genet Mol Res,2016,doi:10.4238/gmr.15038804.
[30]侯志涛,孙忠人,刘松涛,等.电针对缺血性学习记忆障碍大鼠氧自由基及凋亡相关蛋白表达的影响[J].针刺研究,2015,40(6):431-438.
[2]潘天春.四川攀西地区小檗属植物资源及利用价值[J].技术与市场,2013,20(5):314-315.
[3]中国药典[S].三部.2010.
[4]Alamgeer,Naz H,Rasool S,et al.Anti-inflammatory,analgesic and antipyretic activities of the aqueous methanolic extract of Berberis calliobotrys in albino mice[J].Acta Poloniae Pharm,2016,73(3):717-728.
[5]Mahmoudvand H,Sharififar F,Sharifi I,et al.In vitro inhibitory effect of Berberis vulgaris(Berberidaceae)and its main component,berberine against different leishmania species[J].Iranian J Parasitol,2014,9(1):28-36.
[6]Alamgeer,Chabert P,Akhtar M S,et al.Endothelium-independent vasorelaxant effect of a Berberis orthobotrys,root extract via inhibition of phosphodiesterases in the porcine coronary artery[J].Phytomedicine,2016,23(8):793-799.
[7]Alamgeer,Akhtar M S,Jabeen Q,et al.Possible mechanism of cardiac depressant activity of Berberis orthobotrys roots in isolated rabbit heart[J].Acta Poloniae Pharm,2014,71(4):667-675.
[8]Ashraf H,Heidari R,Nejati V.Antihyperglycemic and antihyperlipidemic effects of fruit aqueous extract of Berberis integerrima Bge.in streptozotocin-induced diabetic rats[J].Iranian J Pharm Res,2014,13(4):1313-1318.
[9]Derosa G,Bonaventura A,Bianchi L,et al.Effects of Berberis aristata/Silybum marianum association on metabolic parameters and adipocytokines in overweight dyslipidemic patients[J].J Biol Regul Homeostatic Agents,2013,27(3):717-728.
[10]李端.中药化学(供中药等专业用)[M].北京:人民卫生出版社,2005.
[11]Elmerahbi R,Liu Y N,Eid A,et al.Berberis libanotica ehrenb extract shows anti-neoplastic effects on prostate cancer stem/progenitor cells[J].PLoS One,2014,9(11):e112453.
[12]Bonesi M,Loizzo M R,Conforti F,et al.Berberis aetnensis and B.libanotica:A comparative study on the chemical composition,inhibitory effect on key enzymes linked to Alzheimer's disease and antioxidant activity[J].J Pharm Pharmacol,2013,65(12):1726-1735.
[13]Ahmed S,Shakeel F.Voltammetric determination of antioxidant character in Berberis lycium Royel,Zanthoxylum armatum and Morus nigra Linn.plants[J].Pakistan J Pharm Sci,2012,25(3):501-507.
[14]Albrecht C,Pellarin G,Rojas M J,et al.Beneficial effect of Berberis buxifolia Lam,Ziziphus mistol Griseb and Prosopis alba extracts on oxidative stress induced by chloramphenicol[J].Medicina,2010,70(1):65-70.
[15]Haider S,Saleem S,Perveen T,et al.Age-related learning and memory deficits in rats:Role of altered brain neurotransmitters,acetylcholinesterase activity and changes in antioxidant defense system[J].Age,2014,36(3):1291-1302.
[16]马丽丽,陈晓红.小檗碱在神经系统疾病中的应用进展[J].新医学,2012,43(7):437-440.
[17]李增强.小檗碱治疗糖尿病的肠道作用靶点及分子机制研究[D].沈阳:沈阳药科大学,2012.
[18]王柳卜,贾宪生,胡成刚,等.三颗针中盐酸小檗碱、盐酸药根碱及盐酸巴马汀的含量测定[J].时珍国医国药,2011,22(1):175-177.
[19]李路扬,龙娓芳,万定荣,等.5种小檗属药用植物总生物碱及小檗碱的含量测定[J].中国实验方剂学杂志,2014,20(17):91-94.
[20]李晶.鱼油制剂改善小鼠记忆作用的实验研究[J].食品科学,2004,25(10):301-304.
[21]司茹,郑梦思,邹莉波.美藤果油辅助改善小鼠记忆的功效[J].食品科学,2017,38(9):202-206.
[22]吕姗珊,唐桂英,杨超,等.小儿黄龙颗粒对记忆障碍模型小鼠学习记忆功能的影响[J].中国实验方剂学杂志,2014,20(14):172-177.
[23]明建,曾凯芳,吴素蕊,等.天麻多糖PGEB-3-H对东莨菪碱所致小鼠学习记忆障碍的影响[J].食品科学,2010,31(3):246-249.
[24]陈瑾.血清T-SOD、GSH-Px、MDA、NO、NOS水平与衰老的关系及康寿益胶囊的干预作用[D].重庆:重庆医科大学,2003.
[25]杨伟丽,马志刚,祁梅.甘肃不同产地三颗针不同部位生物碱的含量测定[J].甘肃医药,2009,28(5):349-351.
[26]何丹丹,张磊,刘力,等.骆驼蓬子总生物碱改善小鼠学习记忆能力[J].中成药,2015,37(3):478-482.
[27]王海波,王波,邸学,等.钩藤生物碱部位对AD大鼠学习记忆作用研究[J].时珍国医国药,2017,28(5):1027-1029.
[28]姜琳珊,李菲,聂晶,等.金钗石斛总生物碱对APP/PS1转基因小鼠学习记忆能力的影响[J].遵义医学院学报,2016,39(3):246-249.
[29]An L,Han X,Li H,et al.Effects and mechanism of cerebroprotein hydrolysate on learning and memory ability in mice[J].Genet Mol Res,2016,doi:10.4238/gmr.15038804.
[30]侯志涛,孙忠人,刘松涛,等.电针对缺血性学习记忆障碍大鼠氧自由基及凋亡相关蛋白表达的影响[J].针刺研究,2015,40(6):431-438.