复方白花蛇舌草胶囊抗肿瘤作用的初步研究
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摘要
目的:研究复方白花蛇舌草胶囊的抗肿瘤作用。方法:制备复方白花蛇舌草胶囊含药血清,观察其对体外培养的HepG_2、BEL-7402肿瘤细胞克隆、增殖能力的影响,及对小鼠腹腔巨噬细胞吞噬功能的影响。建立H_(22)和S_(180)荷瘤小鼠模型进行体内抗肿瘤实验,小鼠随机分为荷瘤对照组、氟尿嘧啶组及复方白花蛇舌草胶囊高、中、低剂量组,连续灌胃给药7 d后,观察复方白花蛇舌草胶囊对荷瘤小鼠的抑瘤作用。结果:复方白花蛇舌草胶囊高剂量含药血清可有效抑制HepG_2、BEL-7402细胞的克隆形成能力,提高小鼠腹腔巨噬细胞的吞噬功能,与空白对照组比较有显著性差异(P<0.05或P<0.01)。复方白花蛇舌草胶囊高、中剂量组对H_(22)荷瘤小鼠具有明显的抑瘤作用(P<0.05)。结论:复方白花蛇舌草胶囊具有一定的抗肿瘤作用。
引文
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[2]张文娟,王永华.系统药理学原理、方法及在中医药中的应用[J].世界中医药,2015,2(10):280-286.
[3]Chen X Z,Cao Z Y,Chen T S,et al.Water extract of Hedyotis diffusa Willd.suppresses proliferation of human HepG2cells and potentiates the anticancer efficacy of low-dose 5-fluorouracil by inhibiting the CDK2-E2F1 pathway[J].Oncol Rep,2012,28(2):742-748.
[4]Lee H Z,Bau D T,Kuo C L,et al.Clarification of the phenotypic characteristics and anti-tumor activity of Hedyotis diffusa[J].Am J Chin Med,2011,39(1):201-213.
[5]Li Q,Wang X,Shen A,et al.Hedyotis diffusa Willd.overcomes 5-fluorouracil resistance in human colorectalcancer HCT-8/5-FU cells by downregulating the expression of P-glycoprotein and ATP-binding cassettesubfamily G member 2[J].Exp Ther Med,2015,10(5):1845-1850.
[6]Marconett C N,Morgenstern T J,San R A K,et al.Aphytochemical extract from the Scutellaria barbata plant,disrupts proliferation of human breast and prostate cancercells through distinct mechanisms dependent on thecancer cell phenotype[J].Cancer Biol Ther,2010,10(4):397-405.
[7]Aalinkeel R,Bindukumar B,Reynolds J L,et al.Thedietary bioflavonoid,quercetin,selectively inducesapoptosis of prostate cancer cells by down-regulating theexpression of heat shock protein 90[J].Prostate,2008,68(16):1773-1789.
[8]杨沙,段灿灿,晏仁义,等.基于网络药理学的半枝莲抗肿瘤活性成分及整合作用机制研究[J].中草药,2018,49(15):3471-3482.
[9]方远书,何忠平,张辉,等.元胡止痛胶囊的含药血清对痛经模型动物的影响[J].中国比较医学杂志,2010,20(8):35-37.
[10]杨荣,刘群,康莲莲,等.藏药绿萝花体外抑制肿瘤细胞生长的实验研究[J].中成药,2015,37(9):2061-2065.
[11]Rubio V,Garcia-perez A I,Herraez A,et al.Esculetin modulatescytotoxicity induced by oxidants in NB4 human leukemia cells[J].Exp Toxicol Pathol,2017,69(8):700-712.
[12]师林,柯斌,宋朝阳.加味龟鹿二仙胶汤对气阴两虚肺癌小鼠化疗的减毒增效作用[J].中药材,2014,37(8):1434-1437.
[13]Ushio S,Egashira N,Sada H,et al.Goshajinkigan reduces oxaliplatin-induced peripheral neuropathy without affecting antitumour efficacyin rodents[J].Eur J Cancer,2012,48(9):1407-1413.
[14]Tai J,Cheung S,Wu M,et al.Antiproliferation effect of Rosemary(Rosmarinus oficinalis)on human ovarian cancer cells in vitro[J].Phytomedicine,2012,19(5):436-443.