代谢组学在中药方剂作用机制及药效物质基础研究应用现状
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摘要
中药方剂的药效物质基础是关系到中药有效性及安全性等质量问题的关键因素,其研究方法是制约中药现代化及国际化进程的关键科学问题。中药方剂给药形式的特殊性及方证对应疗效的专属性,决定了其药效物质基础确认的复杂性。因此,寻求一种可靠的方法研究方剂的作用机制及物质基础尤为重要。代谢组学作为一个新兴学科,具有研究生命整体信息的优势,有望成为阐明中药方剂作用机制并挖掘其药效物质基础的可行方法。
The efficacy material basis of traditional Chinese medicine(TCM)is the key factor of quality problems such as efficacy,safety and so on. The research technologys are the critical scientific problems restricted the process of modernization and internationalization of TCM. Due to the particularity of dosage form and the specificity of corresponding effect,the confirm of efficacy material basis of TCM is very complex. Therefore,it's very important to seek a reliable method to study the mechanism and material basis of TCM formula. As an emerging subject,metabolomics has an advantage in the research of overall life information and it may become a feasible way to clarify the mechanism and material basis of TCM.
The efficacy material basis of traditional Chinese medicine(TCM)is the key factor of quality problems such as efficacy,safety and so on. The research technologys are the critical scientific problems restricted the process of modernization and internationalization of TCM. Due to the particularity of dosage form and the specificity of corresponding effect,the confirm of efficacy material basis of TCM is very complex. Therefore,it's very important to seek a reliable method to study the mechanism and material basis of TCM formula. As an emerging subject,metabolomics has an advantage in the research of overall life information and it may become a feasible way to clarify the mechanism and material basis of TCM.
引文
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[25]杨琪伟,杨莉,熊爱珍,等.赤芍和白芍抗炎作用的UPLCMS代谢组学初步研究[J].中国中药杂志,2011,36(6):694-697.
[26]李春雨,王平,王张,等.基于代谢组学技术的大黄治疗慢性肾功能衰竭的作用机制研究[J].中草药,2012,43(2):312-315.
[27]邹忠杰,谢媛媛,龚梦鹃,等.巴戟天补肾阳作用的尿液代谢组学研究[J].药学学报,2013,48(11):1733-1777.
[28]龚梦鹃,叶文华,谢媛媛,等.巴戟天补肾阳作用的血清代谢组学研究[J].中国中药杂志,2012,37(11):1682-1685.
[29]马增春,霍超,周思思,等.四物汤对辐射致血虚证小鼠作用的代谢组学研究[J].中国中药杂志,2012,37(9):1289-1295.
[30]霍超,王穆,马增春,等.辐射损伤所致血虚证小鼠模型及四物汤反证的代谢组学研究[J].天津中医药,2010,27(3):233-235.
[31]王穆,让蔚清,张琪,等.四物汤对环磷酰胺所致血虚证治疗后的代谢组学研究[J].中国中药杂志,2010,35(5):630-634.
[32]谢宝刚,方修忠,陈颖,等.基于HPLC-UV的代谢组学方法对六味地黄丸的抗炎药效评价[J].中国中药杂志,2012,37(17):2635-2638.
[33]姚卫峰,蒋叶娟,蔡秀江,等.二至丸对四氯化碳致大鼠急性肝损伤血浆代谢组学研究[J].中国药学杂志,2013,48(10):808-813.
[34]刘梅森,林汉卿,李钟,等.清伙灵对大鼠积滞化热证干预作用的代谢组学研究[J].中国实验方剂学杂志,2014,20(5):118-122.
[35]王东琴,李晓伟,张福生,等.基于GC-MS代谢组学技术的狭叶柴胡解热作用研究[J].中草药,2013,44(5):574-580.
[36]耿放,张宁,方衡,等.逍遥散对急性肝损伤大鼠模型保护作用的代谢组学研究[J].中药材,2014,37(2):275-279.
[37]Wang X J,Zhang A H,Sun H,et al.Future perspectives of Chinese medical formulae:chinmedomics as an effector[J].OMICS,2012,16(7):414-421.
[38]Wang X,Zhang A,Zhou X,et al.An integrated chinmedomics strategy for discovery of effective constituents from traditional herbal medicine[J].Scientific Reports,2015,6:331-334.
[39]Wang X,Zhang A,Sun H,et al.Discovery and development of innovative drug from traditional medicine by integrated chinmedomics strategies in the post-genomic era[J].Trac Trends in Analytical Chemistry,2016,76:86-94.
[40]Tong X,Sun H,Yan G L,et al.Evaluation Study on Urine Metabolomics in Yinhuang Rat Model Induced by Triplet Factors of Rhubarb,Ethanol,andα-Nephthylisothiolyanate[J].Chin J Integr Med,2011,5(17):369-375.
[41]Wang P,Sun H,Lv H T,et al.Thyroxine and reserpine-induced changes in metabolic profiles of rat urine and the therapeutic effect of Liu Wei Di Huang Wan detected by UPLC-HDMS[J].J Pharm Biomed Anal,2010,53(3):631-645.
[42]Cao H,Zhang A,Sun H,et al.Metabolomics-proteomics profiles delineate metabolic changes in kidney fibrosis disease[J].PROTEOMICS,2015,15(21):3699-3710.
[43]Zhang A,Liu Q,Zhao H,et al.Phenotypic characterization of nanshi oral liquid alters metabolic signatures during disease prevention[J].Scientific Reports,2016,6:523-526.
[44]Yang B,Zhang A,Sun H,et al.Metabolomic study of insomnia and intervention effects of Suanzaoren decoction using ultraperformance liquid-chromatography/electrospray-ionization synapt high-definition mass spectrometry[J].Journal of Pharmaceutical&Biomedical Analysis,2012,58(2):113-124.
[45]Wang X J,Zhang S,Zhang A H,et al.Metabolomics study of type 2 diabetes and therapeutic effects of Tianqijiangtangcapsule using ultra-performance liquid chromatography/electrospray ionization quadruple time-of-flight mass spectrometry[J].Analytical Methods,2013,5:2218-2226.
[46]刘琦,赵宏伟,张爱华,等.基于中医方证代谢组学研究男仕胶囊治疗肾阳虚证的药效物质基础及作用机制[J].中国中药杂志,2016(15):2901-2914.
[2]陶丽,范方田,刘玉萍,等.中药及其组分配伍的整合作用研究实践与进展[J].中国药理学通报,2013,29(2):153-156.
[3]王喜军.中药及中药复方的血清药物化学研究[J].世界科学技术—中药现代化,2002,4(2):1-4.
[4]徐彬,陈永刚,吴金虎,等.中药复方药效物质基础研究的概述[J].医学综述,2014,20(1):126-128.
[5]王元清,严建业,师白梅,等.中药复方药效物质基础研究进展[J].中国中医药信息杂志,2012,19(5):99-102.
[6]周红光,陈海滨,王瑞平,等.代谢组学在中药复方研究中的应用[J].中国药理学通报,2013,29(2):161-165.
[7]Sun H,Zhang A H,Yan G L,et al.Metabolomic analysis of key regulatory metabolites in hepatitis C virus-infected tree shrews[J].Mol Cell Proteomics,2013,12(3):710-719.
[8]Wang X J,Yang B,Sun H,et al.Pattern recognition approaches and computational systems tools for ultra performance liquid chromatography-mass spectrometry-based comprehensive metabolomic profiling and pathways analysis of biological data sets[J].Anal Chem,2012,84(1):428-439.
[9]Wang X J,Yang B,Zhang A H,et al.Potential drug targets on insomnia and intervention effects of Jujuboside A through metabolic pathway analysis as revealed by UPLC/ESI-SYNAPTHDMS coupled with pattern recognition approach[J].J Proteomics,2012,75(4):1411-1427.
[10]Zhang A H,Sun H,Han Y,et al.Ultraperformance liquid chromatography-mass spectrometry based comprehensive metabolomics combined with pattern recognition and network analysis methods for characterization of metabolites and metabolic pathways from biological data sets[J].Anal Chem,2013,85(15):7606-7612.
[11]Zhang A H,Sun H,Wang P,et al.Modern analytical techniques in metabolomics analysis[J].Analyst.2012,137(2):293-300.
[12]Zhang A H,Sun H,Wang Z,et al.Metabolomics:towards understanding traditional Chinese medicine[J].Planta Med,2010,76(17):2026-2035.
[13]许家珍,周帅飞,贾伟,等.四君子汤复方多糖对脾虚小鼠血清代谢组学的影响[J].山东中医杂志,2016,35(10):905-908.
[14]刘志刚,李泽庚,徐彬,等.六味补气胶囊对肺气虚慢性阻塞性肺疾病大鼠模型肺组织代谢物及代谢特征标志物影响代谢组学随机平行对照研究[J].实用中医内科杂志,2015(9):134-139.
[15]Huang D,Yang J,Lu X,et al.An integrated plasma and urinary metabonomic study using UHPLC-MS:Intervention effects of Epimedium koreanum,on“Kidney-Yang Deficiency syndrome”rats[J].Journal of Pharmaceutical&Biomedical Analysis,2013,76:200-206.
[16]Wu X,Tang S,Jin Y,et al.New analytical method for the study of metabolism of swertiamarin in rats after oral administration by UPLC-TOF-MS following DNPH derivatization.[J].Biomedical Chromatography,2015,29(8):1184-1189.
[17]Yang B,Zhang A H,Sun H,et al.Metabolomic study of insomnia and intervention effects of Suanzaoren decoction using ultra-performance liquid-chromatography/electrosprayionization synapt high-definition mass spectrometry[J].Journal of Pharmaceutical and Biomedical Analysis,2012,58:113-124.
[18]Wang X J,Wang Q Q,Zhang A H,et al.Metabolomics study of intervention effects of Wen-Xin-Formula using ultra highperformance liquid chromatography/mass spectrometry coupled with pattern recognition approach[J].Journal of Pharmaceutical and Biomedical Analysis,2013,74:22-30.
[19]Zhang A,Yuan Y,Wang X J,et al.An in vivo analysis of the therapeutic and synergistic properties of Chinese medicinal formula Yin-Chen-Hao-Tang based on its active constituents[J].Fitoterapia,2011,82:1160-1168.
[20]王喜军,孙文军,孙晖,等.茵陈蒿汤不同配伍变化对大鼠血中移行成分的影响[J].中国天然药物,2008,6(1):43-47.
[21]Sun H,Ni B,Wang X J,et al.Metabolomics study on Fuzi and its processed products using ultra-performance liquidchromatography/electrospray-ionization synapt high-definition mass spectrometry coupled with pattern recognition analysis[J].Analyst,2012,137:170-185.
[22]Wang X J,Wang H Y,Zhang A H,et al.Metabolomics Study on the Toxicity of Aconite Root and Its Processed Products Using Ultrap erformance Liquid-Chromatography/ElectrosprayIonization Synapt High-Definition Mass Spectrometry Coupled with Pattern Recognition Approach and Ingenuity Pathways Analysis[J].Journal of Proteome Research,2012,11:1284-1301.
[23]Dong H,Zhang A H,Sun H,et al.Ingenuity pathways analysis of urine metabolomics phenotypes toxicity of Chuanwu in Wistar rats by UPLC-Q-TOF-HDMS coupled with pattern recognition methods[J].Molecular Bio Systems,2012,8(4):1206-1221.
[24]王丽,宋敏,杭太俊,等.NMR代谢组学法研究大蒜辣素对大鼠的作用机制[J].药学学报,2009,44(9):1019-1024.
[25]杨琪伟,杨莉,熊爱珍,等.赤芍和白芍抗炎作用的UPLCMS代谢组学初步研究[J].中国中药杂志,2011,36(6):694-697.
[26]李春雨,王平,王张,等.基于代谢组学技术的大黄治疗慢性肾功能衰竭的作用机制研究[J].中草药,2012,43(2):312-315.
[27]邹忠杰,谢媛媛,龚梦鹃,等.巴戟天补肾阳作用的尿液代谢组学研究[J].药学学报,2013,48(11):1733-1777.
[28]龚梦鹃,叶文华,谢媛媛,等.巴戟天补肾阳作用的血清代谢组学研究[J].中国中药杂志,2012,37(11):1682-1685.
[29]马增春,霍超,周思思,等.四物汤对辐射致血虚证小鼠作用的代谢组学研究[J].中国中药杂志,2012,37(9):1289-1295.
[30]霍超,王穆,马增春,等.辐射损伤所致血虚证小鼠模型及四物汤反证的代谢组学研究[J].天津中医药,2010,27(3):233-235.
[31]王穆,让蔚清,张琪,等.四物汤对环磷酰胺所致血虚证治疗后的代谢组学研究[J].中国中药杂志,2010,35(5):630-634.
[32]谢宝刚,方修忠,陈颖,等.基于HPLC-UV的代谢组学方法对六味地黄丸的抗炎药效评价[J].中国中药杂志,2012,37(17):2635-2638.
[33]姚卫峰,蒋叶娟,蔡秀江,等.二至丸对四氯化碳致大鼠急性肝损伤血浆代谢组学研究[J].中国药学杂志,2013,48(10):808-813.
[34]刘梅森,林汉卿,李钟,等.清伙灵对大鼠积滞化热证干预作用的代谢组学研究[J].中国实验方剂学杂志,2014,20(5):118-122.
[35]王东琴,李晓伟,张福生,等.基于GC-MS代谢组学技术的狭叶柴胡解热作用研究[J].中草药,2013,44(5):574-580.
[36]耿放,张宁,方衡,等.逍遥散对急性肝损伤大鼠模型保护作用的代谢组学研究[J].中药材,2014,37(2):275-279.
[37]Wang X J,Zhang A H,Sun H,et al.Future perspectives of Chinese medical formulae:chinmedomics as an effector[J].OMICS,2012,16(7):414-421.
[38]Wang X,Zhang A,Zhou X,et al.An integrated chinmedomics strategy for discovery of effective constituents from traditional herbal medicine[J].Scientific Reports,2015,6:331-334.
[39]Wang X,Zhang A,Sun H,et al.Discovery and development of innovative drug from traditional medicine by integrated chinmedomics strategies in the post-genomic era[J].Trac Trends in Analytical Chemistry,2016,76:86-94.
[40]Tong X,Sun H,Yan G L,et al.Evaluation Study on Urine Metabolomics in Yinhuang Rat Model Induced by Triplet Factors of Rhubarb,Ethanol,andα-Nephthylisothiolyanate[J].Chin J Integr Med,2011,5(17):369-375.
[41]Wang P,Sun H,Lv H T,et al.Thyroxine and reserpine-induced changes in metabolic profiles of rat urine and the therapeutic effect of Liu Wei Di Huang Wan detected by UPLC-HDMS[J].J Pharm Biomed Anal,2010,53(3):631-645.
[42]Cao H,Zhang A,Sun H,et al.Metabolomics-proteomics profiles delineate metabolic changes in kidney fibrosis disease[J].PROTEOMICS,2015,15(21):3699-3710.
[43]Zhang A,Liu Q,Zhao H,et al.Phenotypic characterization of nanshi oral liquid alters metabolic signatures during disease prevention[J].Scientific Reports,2016,6:523-526.
[44]Yang B,Zhang A,Sun H,et al.Metabolomic study of insomnia and intervention effects of Suanzaoren decoction using ultraperformance liquid-chromatography/electrospray-ionization synapt high-definition mass spectrometry[J].Journal of Pharmaceutical&Biomedical Analysis,2012,58(2):113-124.
[45]Wang X J,Zhang S,Zhang A H,et al.Metabolomics study of type 2 diabetes and therapeutic effects of Tianqijiangtangcapsule using ultra-performance liquid chromatography/electrospray ionization quadruple time-of-flight mass spectrometry[J].Analytical Methods,2013,5:2218-2226.
[46]刘琦,赵宏伟,张爱华,等.基于中医方证代谢组学研究男仕胶囊治疗肾阳虚证的药效物质基础及作用机制[J].中国中药杂志,2016(15):2901-2914.