基于多指标成分的舒胃合剂质量评价
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摘要
目的通过测定芥子碱硫氰酸盐、厚朴酚、和厚朴酚、柚皮苷、橙皮苷和新橙皮苷的含量对舒胃合剂进行质量评价。方法采用反相高效液相色谱法(RP-HPLC),Agilent ZORBAX Eclispse SB-C_(18)色谱柱(4.6 mm×250 mm,5μm),流动相为乙腈-0.1%磷酸溶液梯度洗脱,流速1.0 mL·min~(-1),检测波长分别为326 nm(芥子碱硫氰酸盐),294 nm(厚朴酚、和厚朴酚),283 nm(柚皮苷、橙皮苷、新橙皮苷),柱温30℃。结果芥子碱硫氰酸盐、厚朴酚、和厚朴酚、柚皮苷、橙皮苷和新橙皮苷分别在0.049 6～1.24,0.048 2～1.205,0.060 5～1.512 5,0.187 2～4.68,0.131 6～3.29,0.197～4.925μg线性关系良好; 6种成分平均回收率分别为100.66%,99.86%,101.37%,102.41%,99.01%,102.05%,RSD分别为0.82%,1.89%,2.56%,0.74%,1.54%,0.99%(n=6)。结论该方法简便,准确可靠,重复性好,专属性好,分离效果好,适用于舒胃合剂的质量评价。
Objective The quality evaluation of the Shuwei mixture was determined by the content of the six components of sinapine cyanide sulfonate,magnolol,honokiol,hesperidin,naringin and neohesperidin. Methods RP-HPLC method was used.The separation was performed on a Agilent ZORBAX Eclispse SB-C_(18) column( 4.6 mm× 250 mm,5 mm),the mobile phase consisted of acetonitrile( A)-0. 1% phosphoric acid with gradient elution at the flow rate of 1. 0 mL· min~(-1). The detection wavelength was 326 nm( sinapine cyanide sulfonate),294 nm( magnolol, honokiol) and 283 nm( naringin,neohesperidin). The column temperature was kept at 30 ℃. Results The sinapine cyanide sulfonate,magnolol,honokiol,hesperidin,naringin and neohesperidin all had good linear relationship in the ranges of 0.049 6-1.24,0.048 2-1.205,0.060 5-1.512 5,0.187 2-4.68,0.131 6-3.29,0.197-4.925 μg.The average recoveries were 100.66%,99.86%,101.37%,102.41%,99.01%,102.05%,respectively,RSD were 0.82%,1.89%,2.56 %,0.74%,1.54%,0.99%,respectively. Conclusion The method is simple,accurate,reproducible and nice to the separation,and can be used for the quality evaluation of Shuwei mixture.
Objective The quality evaluation of the Shuwei mixture was determined by the content of the six components of sinapine cyanide sulfonate,magnolol,honokiol,hesperidin,naringin and neohesperidin. Methods RP-HPLC method was used.The separation was performed on a Agilent ZORBAX Eclispse SB-C_(18) column( 4.6 mm× 250 mm,5 mm),the mobile phase consisted of acetonitrile( A)-0. 1% phosphoric acid with gradient elution at the flow rate of 1. 0 mL· min~(-1). The detection wavelength was 326 nm( sinapine cyanide sulfonate),294 nm( magnolol, honokiol) and 283 nm( naringin,neohesperidin). The column temperature was kept at 30 ℃. Results The sinapine cyanide sulfonate,magnolol,honokiol,hesperidin,naringin and neohesperidin all had good linear relationship in the ranges of 0.049 6-1.24,0.048 2-1.205,0.060 5-1.512 5,0.187 2-4.68,0.131 6-3.29,0.197-4.925 μg.The average recoveries were 100.66%,99.86%,101.37%,102.41%,99.01%,102.05%,respectively,RSD were 0.82%,1.89%,2.56 %,0.74%,1.54%,0.99%,respectively. Conclusion The method is simple,accurate,reproducible and nice to the separation,and can be used for the quality evaluation of Shuwei mixture.
引文
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[7]张伟,谢思敏,吴孟华,等.RRLC法同时测定芪术益气润肠颗粒中6种成分含量[J].中药材,2016,39(1):127-130.
[8]周亚萍,陆兔林,毛春芹,等.HPLC法同时测定芪术益气润肠颗粒中6种成分含量[J].中药材,2016,39(1):127-130.
[9]朱立俏,盛华刚,周洪雷.基于指纹图谱考察不同型号大孔树脂对莱菔子水提液的纯化效果[J].中国实验方剂学杂志,2013,19(24):21-24.
[10]刘莉,袁强华,宋英.疏经防痛胶囊的定性定量分析[J].中国实验方剂学杂志,2016,22(12):84-87.
[11]蒋海强,丁臻,周洪雷.高效液相色谱测定莱菔子总碱中芥子碱硫氰酸盐含量[J].中国实验方剂学杂志,2012,18(17):78-80.
[12]张美,熊瑞,崔园园,等.星点设计-响应面法联合正交实验优化配方颗粒平胃散的提取工艺[J].中华中医药杂志,2016,31(12):5307-5311.
[2]程庆兵,徐小梅.高效液相色谱法同时测定益气和胃胶囊中4种成分的含量[J].医药导报,2017,36(1):70-72.
[3]康四和,邓海英.不同生长期枳实枳壳中柚皮苷橙皮苷新橙皮苷的含量研究[J].时珍国医国药,2012,23(8):1918-1919.
[4]国家中医药管理局《中华本草》编委会.中华本草[M].上海:上海科学技术出版社,1999:729.
[5]沈亚芬,孙元龙.沈金银.中药莱菔子药理作用研究进展[J].中国中医药科技,2011,18(3):271-272.
[6]赵婧,霍青,李运伦.莱菔子的现代研究及临床应用[J].长春中医药大学学报,2011,27(2):294-296.
[7]张伟,谢思敏,吴孟华,等.RRLC法同时测定芪术益气润肠颗粒中6种成分含量[J].中药材,2016,39(1):127-130.
[8]周亚萍,陆兔林,毛春芹,等.HPLC法同时测定芪术益气润肠颗粒中6种成分含量[J].中药材,2016,39(1):127-130.
[9]朱立俏,盛华刚,周洪雷.基于指纹图谱考察不同型号大孔树脂对莱菔子水提液的纯化效果[J].中国实验方剂学杂志,2013,19(24):21-24.
[10]刘莉,袁强华,宋英.疏经防痛胶囊的定性定量分析[J].中国实验方剂学杂志,2016,22(12):84-87.
[11]蒋海强,丁臻,周洪雷.高效液相色谱测定莱菔子总碱中芥子碱硫氰酸盐含量[J].中国实验方剂学杂志,2012,18(17):78-80.
[12]张美,熊瑞,崔园园,等.星点设计-响应面法联合正交实验优化配方颗粒平胃散的提取工艺[J].中华中医药杂志,2016,31(12):5307-5311.