以药效学为指标对苓仙降酸复方提取工艺的筛选
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摘要
目的:以药效学为指标对苓仙降酸复方的不同工艺提取物进行比较研究,筛选出最佳提取工艺。方法:通过对Wistar大鼠灌胃腺嘌呤和氧嗪酸钾建立高尿酸血症模型,将40只建模成功的大鼠随机分为模型组,苓仙降酸复方水提组(10g生药/kg)、水提醇沉组(10g生药/kg)、醇提组(10g生药/kg),阳性对照别嘌醇组(20mg/kg),另设空白对照组。每组均灌胃给药,qd,共21d。于第7、14、21天由大鼠眼眶取血测定尿酸(UA)、尿素氮(BUN)、血肌酐(CR)水平,第21天取血后处死大鼠,取其脏器,测定肝脏中腺苷脱氨酶(ADA)水平和称量肾脏重量。结果:与模型组比较,苓仙降酸复方水提组大鼠的UA、BUN、CR、肾脏重量和ADA水平均显著降低(P<0.05,P<0.01),而水提醇沉组和醇提组的各项指标部分有显著差异,部分仅有降低趋势。结论:苓仙降酸复方水提物降UA及缓解肾脏病理损伤的作用优于水提醇沉物和醇提物,其最佳提取工艺为水提取。
Objective:To study the extracts from Lingxianjiangsuan compound by different extraction methods with pharmacodynamics as an index,so as to screen out the optimal extraction method.Methods:The Wistar rats were gavaged with adenine and potassium oxonate to establish hyperuricemia models,and then upon successful establishment of the model,40 rats were randomly divided into the model group,the Lingxianjiangsuan compound water extraction group,the water extraction and alcohol precipitation group,the alcohol extraction group(10 g crude drugs/kg),the positive controlled allopurinol treatment group(20 mg/kg)and the blank control group.The drugs were administered by gavage once daily for 21 d.The levels of uric acid(UA),urea nitrogen(BUN),serum creatinine(CR)were determined on days 7,14 and 21,respectively.On day 21,the rats were excuted,after blood samples were taken,and viscera were obtained.Then the level of adenosine deaminase(ADA)in liver and kidney weight were measured.Results:Compared with those of the model group,the levels of UA,BUN and CR,kidney weight,level of ADA in liver of the water extraction group were significantly decreased(P<0.05,P<0.01),while some indicators in the water extraction and alcohol precipitation group and the alcohol extraction group were significantly difference,and some indicators showed a decreasing trend.Conclusion:The water extract of Lingxianjiangsuan compound has better effects on the reduction of UA and alleviation of pathological damage to kidney,as compared with the water extraction and alcohol precipitation and alcohol extraction,and the optimal extraction method of Lingxianjiangsuan compound is water extraction.
Objective:To study the extracts from Lingxianjiangsuan compound by different extraction methods with pharmacodynamics as an index,so as to screen out the optimal extraction method.Methods:The Wistar rats were gavaged with adenine and potassium oxonate to establish hyperuricemia models,and then upon successful establishment of the model,40 rats were randomly divided into the model group,the Lingxianjiangsuan compound water extraction group,the water extraction and alcohol precipitation group,the alcohol extraction group(10 g crude drugs/kg),the positive controlled allopurinol treatment group(20 mg/kg)and the blank control group.The drugs were administered by gavage once daily for 21 d.The levels of uric acid(UA),urea nitrogen(BUN),serum creatinine(CR)were determined on days 7,14 and 21,respectively.On day 21,the rats were excuted,after blood samples were taken,and viscera were obtained.Then the level of adenosine deaminase(ADA)in liver and kidney weight were measured.Results:Compared with those of the model group,the levels of UA,BUN and CR,kidney weight,level of ADA in liver of the water extraction group were significantly decreased(P<0.05,P<0.01),while some indicators in the water extraction and alcohol precipitation group and the alcohol extraction group were significantly difference,and some indicators showed a decreasing trend.Conclusion:The water extract of Lingxianjiangsuan compound has better effects on the reduction of UA and alleviation of pathological damage to kidney,as compared with the water extraction and alcohol precipitation and alcohol extraction,and the optimal extraction method of Lingxianjiangsuan compound is water extraction.
引文
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[2]马力.高尿酸血症和代谢综合征[J].中国医刊,2010,45(8):18-19.MA Li.Hyperuricemia and metabolic syndrome[J].Chin J Med,2010,45(8):18-19.In Chinese.
[3]周启蒙,杜冠华.治疗高尿酸血症相关药物研究进展[J].中国新药杂志,2016,25(18):2129-2135.ZHOU QiMeng,DU GuanHua.Progress in the research of drugs for the treatment of hyperuricemia[J].Chin J New Drugs,2016,25(18):2129-2135.In Chinese with English abstract.
[4] WANG Ming,ZHAO Jing,ZHANG Nan,et al.Astilbin improves potassium oxonate-induced hyperuricemia and kidney injury through regulating oxidative stress and inflammation response in mice[J].Biomed Pharmacother,2016,83(6):975-988.
[5]王建平,傅旭春.土茯苓降血尿酸乙醇提取物的提取工艺研究[J].中华中医药学刊,2012,30(6):1382-1384.WANG JianPing,FU XuChun.Research on extraction technology of ethanol extract from oriental bittersweet for reducing blood uric acid[J].Chin Arch Tradit Chin Med,2012,30(6):1382-1384.In Chinese with English abstract.
[6]王晓梅,赵岩,赵卫星.痛风宁部分提取物对黄嘌呤氧化酶的抑制作用[J].安徽中医学院学报,2012,31(5):56-58.WANG XiaoMei,ZHAO Yan,ZHAO WeiXing.Inhibitory effects of some extracts of Tongfengning on xanthine oxidase[J].J Anhui Tradit Chin Med Coll,2012,31(5):56-58.In Chinese with English abstract.
[7]钱莺,傅旭春,王建平,等.车前草降血尿酸有效成分提取工艺的研究[J].浙江大学学报(理学版),2010,37(5):560-562,567.QIAN Ying,FU XuChun,WANG JianPing,et al.Study on the extraction process of active ingredients with hypouricuria effect from Plantaginis Herba[J].J Zhejiang Univ(Sci Ed),2010,37(5):560-562,567.In Chinese with English abstract.
[8]王元清,严建业,师白梅,等.中药复方药效物质基础研究进展[J].中国中医药信息杂志,2012,19(5):99-102.WANG YuanQing,YAN JianYe,SHI BaiMei,et al.Progress in research on pharmacodynamic basis of compound traditional Chinese medicine[J].Chin J Inf Tradit Chin Med,2012,19(5):99-102.In Chinese.
[9]任钧国,刘建勋.中药复方新药组方设计思路与方法[J].中国中药杂志,2015,40(17):3413-3415.REN JunGuo,LIU JianXun.Drug design ideas and methods of Chinese herb prescriptions[J].China J Chin Materia Medica,2015,40(17):3413-3415.In Chinese with English abstract.