摘要
目的探究沉默细胞膜锚蛋白(ANK2)基因表达对人胃癌细胞SGC-7901增殖、侵袭、细胞周期的影响及作用机制。方法实验分为对照组、无义组、siRNA-ANK2组,胃癌细胞SGC-7901转染siRNA-ANK2或siRNA无义序列,蛋白质印迹法(Western blotting)检测细胞中ANK2蛋白的水平。CCK-8法检测细胞的活性,流式细胞术检测细胞周期变化,Transwell法检测细胞的侵袭力。结果 SGC-7901细胞转染siRNA-ANK2后,与对照组相比,siRNA-ANK2组ANK2蛋白表达量显著降低(P<0.05),细胞活性明显降低(P<0.05);siRNA-ANK2组细胞周期G0/G1期较对照组显著升高,S期降低(P<0.05),侵袭数目明显降低(P<0.05)。结论沉默ANK2基因抑制胃癌细胞SGC-7901增殖和转移,阻碍细胞周期G1向S期转换。
Objective To investigate the effect of silencing cell membrane ankyrin B(ANK2) gene expression on the proliferation,invasion and cell cycle of human gastric cancer cell line SGC-7901 and its mechanism. Methods The experiments were divided into control group,nonsense group and siRNA-ANK2 group. Gastric cancer cell SGC-7901 was transfected into siRNA-ANK2 or siRNA nonsense sequence. The expression of ANK2 protein was detected by Western blotting. The cell activity was detected by CCK-8 assay. Flow cytometry was used to detect cell cycle changes. Transwell method was used to detect cell invasiveness. Results Compared with the control group,the expression of ANK2 protein in siRNA-ANK2 group was decreased significantly after transfected into siRNA-ANK2( P < 0. 05),cell activity was decreased significantly( P < 0. 05). The G0/G1 phase of siRNA-ANK2 group was significantly higher than that of the control group,S phase was decreased( P < 0. 05),and the number of invasion was significantly decreased( P <0. 05). Conclusion Silencing ANK2 gene can inhibit the proliferation,induce metastasis of gastric cancer cell line SGC-7901,and hinder the transition from cell cycle G1 to S phase.
引文
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