银屑平丸对BALB/c小鼠银屑病样模型血清中IL-6与IL-21表达的影响
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摘要
目的:探究银屑平丸对BALB/c小鼠银屑病样模型血清中IL-6与IL-21表达的影响。方法:将30只BALB/c小鼠随机分为正常组、模型组、雷公藤组、银屑平丸高剂量组及低剂量组,每组6只。除正常组小鼠背部皮肤每日涂抹1次凡士林乳膏外,其余各组小鼠均每日外涂1次咪喹莫特乳膏,连续8 d。造模期间,各组小鼠同时进行灌胃给药:正常组与模型组每日用生理盐水灌胃1次,0.4 mL/次;雷公藤组每日用雷公藤多苷溶液药液灌胃1次,0.4 mL/次;银屑平丸高、低剂量组则每日分别用不同浓度的银屑平丸溶液药液灌胃1次,0.4 mL/次。每日给药24 h后观察各组小鼠背部皮肤的校正PASI评分;末次给药24 h后取血与皮肤组织。通过光镜下观察各组小鼠皮损组织形态学的改变,检测小鼠血清中IL-6与IL-21表达。结果:正常组的校正PASI评分趋势一直为0,其余各组的评分均为上升趋势。模型组、银屑平丸低剂量组、雷公藤组、银屑平丸高剂量组的PASI评分依次降低。模型组小鼠皮损组织为典型的银屑病样病理改变,除正常组小鼠无皮损外,其余各组小鼠均出现不同程度的角化过度及角化不全的病理改变。银屑平丸高剂量组小鼠血清IL-6、IL-21水平低于雷公藤组与银屑平丸低剂量组;雷公藤组小鼠血清IL-6、IL-21水平低于银屑平丸低剂量组。结论:银屑平丸可抑制BALB/c小鼠银屑病模型血清中IL-6与IL-21表达,这可能是银屑平丸治疗银屑病的作用机制之一。
Objective:To explore the influence of Yinxieping pills on serum IL-6 and IL-21 in the BALE/c mice psoriases model.Methods:A total of 30 BALB/c mice were randomLy divided into normal group,model group Leigongteng(雷公藤) group,Yinxieping pills high dose group and low dose group.Except that mice back skin of the normal group were coated with Vaseline cream once a day,the rest of the mice were coated with imiquimod cream daily for 8 days.During model establishment,all mice were intragastric administration on the same time:the normal group and the model group were treated with normal saline once a day by 0.4 mL/times;the Leigongteng group was treated with Leigongteng polygene solution by 0.4 mL/times;Yinxieping pills high and low dose groups were daily treated with different concentrations of Yinxieping pills solution by 0.4 mL/times.The corrected PASI score of the dorsal skin of each group was observed after 24 hours of the daily administration.Blood and skin tissue were collected after 24 hours of the last administration,and the results were finally obtained by light microscopy.The changes of IL-6 and IL-21 in the serum of mice were observed.Results:Except that the corrected PASI score trend of the normal group was always 0,the scores of the other groups arised.The PASI scores of model group,low dose group,Leigongteng group and high dose group were decreased in turn.The pathological changes of the psoriasis-like pathogens were observed in the model group.But the skin of the normal group unchanged.The mice skin in the other groups showed different degrees of hyperkeratosis and parakeratosis.Expression levels of IL-6 and IL-21 in the high dose group was lower than those in Leigongteng group and the Yinxieping pills low dose group,while theose in Leigongteng group was slower than those in the low dose group.Conclusion:It is one of the possible mechanisms that Yinxieping pills control psoriasis by inhibiting serum IL-6 and IL-21.
Objective:To explore the influence of Yinxieping pills on serum IL-6 and IL-21 in the BALE/c mice psoriases model.Methods:A total of 30 BALB/c mice were randomLy divided into normal group,model group Leigongteng(雷公藤) group,Yinxieping pills high dose group and low dose group.Except that mice back skin of the normal group were coated with Vaseline cream once a day,the rest of the mice were coated with imiquimod cream daily for 8 days.During model establishment,all mice were intragastric administration on the same time:the normal group and the model group were treated with normal saline once a day by 0.4 mL/times;the Leigongteng group was treated with Leigongteng polygene solution by 0.4 mL/times;Yinxieping pills high and low dose groups were daily treated with different concentrations of Yinxieping pills solution by 0.4 mL/times.The corrected PASI score of the dorsal skin of each group was observed after 24 hours of the daily administration.Blood and skin tissue were collected after 24 hours of the last administration,and the results were finally obtained by light microscopy.The changes of IL-6 and IL-21 in the serum of mice were observed.Results:Except that the corrected PASI score trend of the normal group was always 0,the scores of the other groups arised.The PASI scores of model group,low dose group,Leigongteng group and high dose group were decreased in turn.The pathological changes of the psoriasis-like pathogens were observed in the model group.But the skin of the normal group unchanged.The mice skin in the other groups showed different degrees of hyperkeratosis and parakeratosis.Expression levels of IL-6 and IL-21 in the high dose group was lower than those in Leigongteng group and the Yinxieping pills low dose group,while theose in Leigongteng group was slower than those in the low dose group.Conclusion:It is one of the possible mechanisms that Yinxieping pills control psoriasis by inhibiting serum IL-6 and IL-21.
引文
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[13]胡文娟,闫玉红,陈海明,等.银屑病模型小鼠肠道Th17细胞及相关细胞因子的研究[J].广东医学,2016,37(13):1925-1928.
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[15]薛国辉,华琳,刘晓峰,等.外周血循环滤泡辅助性T细胞及IL-21在银屑病中的表达及意义[J].中国免疫学杂志,2016,32(11):1645-1648.
[16]王英杰,刘杰,罗瑞静.Th17细胞及其相关因子在银屑病中西医治疗中的研究现状[J].陕西中医,2016,37(2):251-253.
[2]Schut C,Muhl S,Reinisch K,et al.Agreeableness and Self-Consciousness as Predictors of Induced Scratching and Itch in Patients with Psoriasis[J].Int J Behav Med,2015,22(6):726-734.
[3]Zheng Y,Danilenko D M,Valdez P,et al.Interleukin-22,a T(H)17 cytokine,mediates IL-23-induced dermal inflammation and acanthosis[J].Nature,2007,445(7128):648-651.
[4]景海霞,段德鉴.Th17、Th22细胞与银屑病关系的研究进展[J].中国麻风皮肤病杂志,2013,29(10):648-651.
[5]Qin S,Wen J,Bai XC,et al.Endogenous n-3 polyunsaturated fatty acids protect against imiquimod-induced psoriasis-like inflammation via the IL-17/IL-23 axis[J].Mol Med Rep,2014,9(6):2097-2104.
[6]李小鹏,席建元,谢汶芳,等.银屑平丸对豚鼠银屑病样模型VEGF和TNF-α表达的影响[J].中国中西医结合皮肤性病学杂志,2014,13(5):294-297.
[7]席建元,张亚南,李小鹏,等.凉血养阴法对寻常型银屑病患者外周血VEGF和OPN表达的影响[J].中国中西医结合皮肤性病学杂志,2014,13(4):213-216.
[8]韩根成,沈倍奋.Th17细胞分化、调节及效应研究进展[J].生物化学与生物物理进展,2008,35(2):117-122.
[9]叶萍,黄伟林,郑力.Th17细胞相关因子与寻常型银屑病的相关性研究[J].重庆医学,2013,42(15):1706-1710.
[10]Zaretsky M,Etzyoni R,Kaye J,et al.Directed evolution of a soluble human IL-17A receptor for the inhibition of psoriasis plaque formation in a mouse mode[J].Chem Biol,2013,20(2):202-211.
[11]唐娟,张晓艳,宋佩华.Th17细胞相关因子与寻常性进行期银屑病的相关性研究[J].实用皮肤病学杂志,2014,7(3):190-192.
[12]范斌,李峰,李欣,等.血热证银屑病患者Th17细胞与相关转导因子的表达[J].时珍国医国学,2012,23(10):2612-2614.
[13]胡文娟,闫玉红,陈海明,等.银屑病模型小鼠肠道Th17细胞及相关细胞因子的研究[J].广东医学,2016,37(13):1925-1928.
[14]赵枫姝,窦骏.免疫平衡的重要调节者白细胞介素-21[J].生物技术通讯,2008,19(5):715-717.
[15]薛国辉,华琳,刘晓峰,等.外周血循环滤泡辅助性T细胞及IL-21在银屑病中的表达及意义[J].中国免疫学杂志,2016,32(11):1645-1648.
[16]王英杰,刘杰,罗瑞静.Th17细胞及其相关因子在银屑病中西医治疗中的研究现状[J].陕西中医,2016,37(2):251-253.