健脾调肝饮对单纯性肥胖症小鼠核心体温/自主活动度及白色脂肪棕色化的影响研究
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摘要
目的:探讨健脾调肝饮对单纯性肥胖症的作用及机制。方法:将40只C57BL/6J小鼠随机分为空白组10只与模型组30只,模型组通过高脂饲料的喂养造模后,随机分为中药组与肥胖对照组。空白组与肥胖对照组给予生理盐水灌胃,中药组给予中药健脾调肝饮灌胃,实验周期共10周。实验结束时,置入自主活动度/核心体温无线讯号发射器并留取白色脂肪组织,对比3组小鼠自主活动度、核心体温、白色脂肪棕色化特异性标志物UCP-1/PGC-1α等方面指标。结果:10周后,与肥胖对照组相比中药组体质量显著降低;自主活动度及核心体温全天均有所提高;白色脂肪组织中UCP-1、PGC-1α蛋白及mRNA表达水平显著增高。结论:健脾调肝饮可有效减轻肥胖小鼠体质量,提高肥胖小鼠自主活动度及核心体温,提高基础代谢率,上调脂肪组织UCP-1、PGC-1α蛋白及mRNA表达,其可能作用机制为通过增高小鼠白色脂肪棕色化程度,促进产热消耗能量以达到减重的治疗效果。
Objective: To observe the effect and mechanism of Jianpi Tiaogan Yin on simple obesity. Methods: Forty C57 BL/6 J mice were randomly divided into 2 groups: blank group and model group. Model group were given high-fat diet to build simple obesity mice model. The simple obesity mice were randomly divided into 2 groups:Jianpi Tiaogan Yin group( JPTGY) and Control group. The Control group and blank group were given normal saline. JPTGY group were given Jianpi Tiaogan Yin. At the end of the experiment,the activity/core-temperature wireless signal transmitter was placed and the white adipose tissue was collected. We compared the differences in the activity,the core-temperature and the browning of white adipose marker UCP-1/PGC-1α mRNA expression changes among groups. Results: After 10 weeks,compared with the Control group,the body weight of the JPTGY group was significantly decreased. The activity/core-temperature increased all day. The expressions of UCP-1 and PGC-1α protein/mRNA in white adipose tissue were significantly increased. Conclusion: Jianpi Tiaogan Yin can effectively reduce body weight and increase the activity/core-temperature of the obese mice. Jianpi Tiaogan Yin also can increase the basal metabolic rate and the expressions of UCP-1 and PGC-1α mRNA in adipose tissue. The possible mechanism is to increase the browning of white adipose and promote the heat consumption of energy,and then achieving the effect of weight loss.
Objective: To observe the effect and mechanism of Jianpi Tiaogan Yin on simple obesity. Methods: Forty C57 BL/6 J mice were randomly divided into 2 groups: blank group and model group. Model group were given high-fat diet to build simple obesity mice model. The simple obesity mice were randomly divided into 2 groups:Jianpi Tiaogan Yin group( JPTGY) and Control group. The Control group and blank group were given normal saline. JPTGY group were given Jianpi Tiaogan Yin. At the end of the experiment,the activity/core-temperature wireless signal transmitter was placed and the white adipose tissue was collected. We compared the differences in the activity,the core-temperature and the browning of white adipose marker UCP-1/PGC-1α mRNA expression changes among groups. Results: After 10 weeks,compared with the Control group,the body weight of the JPTGY group was significantly decreased. The activity/core-temperature increased all day. The expressions of UCP-1 and PGC-1α protein/mRNA in white adipose tissue were significantly increased. Conclusion: Jianpi Tiaogan Yin can effectively reduce body weight and increase the activity/core-temperature of the obese mice. Jianpi Tiaogan Yin also can increase the basal metabolic rate and the expressions of UCP-1 and PGC-1α mRNA in adipose tissue. The possible mechanism is to increase the browning of white adipose and promote the heat consumption of energy,and then achieving the effect of weight loss.
引文
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[13]何旭云,贺姣姣,郑宁宁,等.黄芪多糖对肥胖小鼠的减肥作用与调节肠道菌群的关系研究[J].世界中医药,2016,11(11):2379-2384,2388.
[14]李晓,张佳琪,王雪,等.黄芪对饮食诱导肥胖大鼠脂肪蓄积及瘦素抵抗的影响[J].中华中医药杂志,2016,31(3):833-837.
[15]Bin Ke,Xiao Ke,Xuesi Wan,et al. Astragalus polysaccharides attenuates TNF-α-induced insulin resistance via suppression of miR-721 and activation of PPAR-γand PI3K/AKT in 3T3-L1 adipocytes[J]. American Journal of Translational Research,2017,9(5):2195.
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[18]游昕,熊大国,郭志斌,等.茯苓多种化学成分及药理作用的研究进展[J].安徽农业科学,2015,43(2):106-109.
[19]岳嘉,常燕琴,蔺美玲,等.丹参对去卵巢肥胖大鼠的影响[J].中医研究,2016,29(4):63-66.
[20]张明发,沈雅琴.薏苡仁抗代谢综合征的药理作用研究进展[J].药物评价研究,2014,37(2):178-183.
[21]宋钦兰,姜萍,张佳琪,等.补脾与醒脾药物对饮食诱导肥胖大鼠瘦素抵抗的调节作用[J].时珍国医国药,2015,26(1):1-3.
[22]Idehen E,Tang Y,Sang S. Bioactive phytochemicals in barley[J]. Journal of Food&Drug Analysis,2017,25(1):148-161.
[23]孙红爽,乜春城,朱小丽,等.大黄素对胰岛素抵抗大鼠脂肪组织11β-羟基类固醇脱氢酶1表达的影响[J].安徽农业大学学报,2016,43(3):427-430.
[24]Jang J,Jung Y,Chae S,et al. Gangjihwan,a polyherbal composition,inhibits fat accumulation through the modulation of lipogenic transcription factors SREBP1C,PPARγand C/EBPα[M]. Journal of Ethnopharmacology,2017.
[25]孙武千,方灵,程丹,等.天然植物复方提取液对摄食高脂饲料小鼠的降脂减肥作用[J].中国食品学报,2016,16(4):25-29.
[2]冯博,吕鸿飞,冯维华,等.健脾调肝饮对单纯性肥胖合并IGR大鼠血清Nesfatin-1含量及Nesfatin-1mRNA在胃部组织表达的影响[J].世界中西医结合杂志,2016,11(6):741-745.
[3]Di C M,Bentham J,Stevens G A,et al. Trends in adult bodymass index in 200 countries from 1975 to 2014:a pooled analysis of 1698 population-based measurement studies with 19. 2 million participants[J]. Lancet,2016,387(10026):1377.
[4]Wang Q A,Tao C,Gupta R K,et al. Tracking adipogenesis during white adipose tissue development,expansion and regeneration[J]. Nature medicine,2013,19(10):1338-1344.
[5]Wang C,Wang L,Li W,et al. Irisin has no effect on lipolysis in 3T3-L1 adipocytes or fatty acid metabolism in Hep G2 hepatocytes[J]. Endocrine,2015,49(1):90-96.
[6]Nedergaard J,Cannon B. The browning of white adipose tissue:some burning issues[J]. Cell metabolism,2014,20(3):396-407.
[7]Lo K A,Sun L. Turning WAT into BAT:a review on regulators controlling the browning of white adipocytes[J]. Bioscience Reports,2013,33(5):711-719.
[8]Crane J D,Palanivel R,Mottillo E P,et al. Inhibiting peripheral serotonin synthesis reduces obesity and metabolic dysfunction by promoting brown adipose tissue thermogenesis[J]. Nature medicine,2015,21(2):166-172.
[9]刘喜明,仝小林,王朋倩.试论“膏浊”致病论[J].世界中西医结合杂志,2009,4(12):839-842.
[10]仝小林,刘文科.论膏浊病[J].中医杂志,2011,52(10):816-818.
[11]李春桂,曹柏龙,苗桂珍,等.健脾祛湿化痰降浊方为主治疗肥胖型糖尿病前期的临床观察[J].陕西中医,2016,37(8):1021-1022.
[12]杨立宏,李靖,王红.中医健脾补肾祛瘀降浊法治疗肥胖的疗效研究[J].时珍国医国药,2015(1):135-136.
[13]何旭云,贺姣姣,郑宁宁,等.黄芪多糖对肥胖小鼠的减肥作用与调节肠道菌群的关系研究[J].世界中医药,2016,11(11):2379-2384,2388.
[14]李晓,张佳琪,王雪,等.黄芪对饮食诱导肥胖大鼠脂肪蓄积及瘦素抵抗的影响[J].中华中医药杂志,2016,31(3):833-837.
[15]Bin Ke,Xiao Ke,Xuesi Wan,et al. Astragalus polysaccharides attenuates TNF-α-induced insulin resistance via suppression of miR-721 and activation of PPAR-γand PI3K/AKT in 3T3-L1 adipocytes[J]. American Journal of Translational Research,2017,9(5):2195.
[16]刘洋,庞敏.大柴胡汤治疗高脂血症概况[J].实用中医内科杂志,2017,31(6):86-89.
[17]Kim S O,Park J Y,Jeon S Y,et al. Saikosaponin a,an active compound of Radix Bupleuri,attenuates inflammation in hypertrophied 3T3-L1 adipocytes via ERK/NF-κB signaling pathways[J]. International Journal of Molecular Medicine,2015,35(4):1126.
[18]游昕,熊大国,郭志斌,等.茯苓多种化学成分及药理作用的研究进展[J].安徽农业科学,2015,43(2):106-109.
[19]岳嘉,常燕琴,蔺美玲,等.丹参对去卵巢肥胖大鼠的影响[J].中医研究,2016,29(4):63-66.
[20]张明发,沈雅琴.薏苡仁抗代谢综合征的药理作用研究进展[J].药物评价研究,2014,37(2):178-183.
[21]宋钦兰,姜萍,张佳琪,等.补脾与醒脾药物对饮食诱导肥胖大鼠瘦素抵抗的调节作用[J].时珍国医国药,2015,26(1):1-3.
[22]Idehen E,Tang Y,Sang S. Bioactive phytochemicals in barley[J]. Journal of Food&Drug Analysis,2017,25(1):148-161.
[23]孙红爽,乜春城,朱小丽,等.大黄素对胰岛素抵抗大鼠脂肪组织11β-羟基类固醇脱氢酶1表达的影响[J].安徽农业大学学报,2016,43(3):427-430.
[24]Jang J,Jung Y,Chae S,et al. Gangjihwan,a polyherbal composition,inhibits fat accumulation through the modulation of lipogenic transcription factors SREBP1C,PPARγand C/EBPα[M]. Journal of Ethnopharmacology,2017.
[25]孙武千,方灵,程丹,等.天然植物复方提取液对摄食高脂饲料小鼠的降脂减肥作用[J].中国食品学报,2016,16(4):25-29.