模板中CGC~+数量对纳米银铂簇性能影响的探讨
|
摘要
对四种含不同数量胞嘧啶-鸟嘌呤-胞嘧啶三连体(CGC~+)的银铂双金属纳米簇的结构及催化活性进行研究,探讨模板中CGC~+数量对其性能的影响.方法以含不同数量CGC~+的DNA三倍体为模板合成四种银铂纳米簇,采用透射电镜、紫外可见分光光度、荧光光谱等方法,研究模板中CGC~+数量对纳米簇结构及催化活性的影响.结果表明,模板中CGC~+数量可影响纳米簇结构,四种簇均有类过氧化物酶活性,能催化H_2O_2氧化TMB,且活性随CGC~+数量增加而有所增强.该研究可为纳米簇的合成及应用提供新思路和参考.
The structure and catalytic activity of four kinds of triplex silver-platinum nanoclusters(triplex-AgPtNCs)containing different amounts of CGC~+were studied, and the effect of CGC~+number on the properties was discussed. Templated by the triplex DNA, four kinds of triplex-AgPtNCs were synthesized, respectively. Then the structure and catalytic activity of these triplex-AgPtNCs were studied by ultraviolet-visible spectrophotometry, fluorescence spectrometry and transmission electron microscope. Results showed the four kinds of triplex silver platinum nanoparticles had peroxidase-like activity, which can catalyze the oxidation of 3,3′,5,5′-tetramethylbenzidine by H_2O_2. Moreover, the catalytic activity had been enhanced along with the increase of the number of CGC~+. Meanwhile, it was also found that the number of CGC~+in template sequences would affect the uniformity of nanoclusters. The study may provide new train of thought or reference for the synthesis and application of nanoclusters.
The structure and catalytic activity of four kinds of triplex silver-platinum nanoclusters(triplex-AgPtNCs)containing different amounts of CGC~+were studied, and the effect of CGC~+number on the properties was discussed. Templated by the triplex DNA, four kinds of triplex-AgPtNCs were synthesized, respectively. Then the structure and catalytic activity of these triplex-AgPtNCs were studied by ultraviolet-visible spectrophotometry, fluorescence spectrometry and transmission electron microscope. Results showed the four kinds of triplex silver platinum nanoparticles had peroxidase-like activity, which can catalyze the oxidation of 3,3′,5,5′-tetramethylbenzidine by H_2O_2. Moreover, the catalytic activity had been enhanced along with the increase of the number of CGC~+. Meanwhile, it was also found that the number of CGC~+in template sequences would affect the uniformity of nanoclusters. The study may provide new train of thought or reference for the synthesis and application of nanoclusters.
引文
[1]Rao CNR,Cheetham A K.Science and technology of nanomaterials:current status and future prospects[J].J.Mater.Chem.,2001,11(12):2887-2894.
[2]Gao LZ,Zhuang J,Nie L,et al.Intrinsic peroxidase-like activity of ferromagnetic nanoparticles[J].Nat.Nanotechnol.,2007,2(9):577-583.
[3]Li RM,Zhen MM,Guan MR,et al.A novel glucose colorimetric sensor based on intrinsic peroxidase-like activity of C60-carboxyfullerenes[J].Biosens.Bioelectron.,2013(47):502-507.
[4]Lan J,Xu W,Wan Q,et al.Colorimetric determination of sarcosine in urine samples of prostatic carcinoma by mimic enzyme palladium nanoparticles[J].Anal.Chim.Acta,2014,825(12):63-68.
[5]Dalui A,Pradhan B,Thupakula U,et al.Insight into the mechanism revealing the peroxidase mimetic catalytic activity of quaternary CuZnFeS nanocrystals:colorimetric biosensing of hydrogen peroxide and glucose[J].Nanoscale,2015(19):9062-9074.
[6]Tian,L,Qi JX,Oderinde O,et al.Planar intercalated copper(II)complex molecule as small molecule enzyme mimic combined with Fe3O4nanozyme for bienzyme synergistic catalysis applied to the microRNA biosensor[J].Biosens.Bioelectron.,2018(110):110-117.
[7]张雅云,钟炎妹,李顺兴,等.基于纳米材料及碳量子点对乙醇的电催化研究[J].闽南师范大学学报(自然科学版),2014,83(1):78-83.
[8]Fu XM,Liu ZJ,Cai SX,et al.Electrochemical aptasensor for the detection of vascular endothelial growth factor(VEGF)based on DNA-templated Ag/Pt bimetallic nanoclusters[J].Chin.Chem.Lett,27,2016:920-926.
[9]Feng J,Huang P,Wu FY.Gold-platinum bimetallic nanoclusters with enhanced peroxidase-like activity and their integrated agarose hydrogel-based sensing platform for the colorimetric analysis of glucose levels in serum[J].Analyst,2017,142(21):4106-4115.
[10]Song Q,Shi Y,He D,et al.Sequence dependent dsDNA-templated formation of fluorescent copper nanoparticles[J]Chemistry,2015,21(6):2417-2422.
[11]Feng L,Huang Z,Ren J,et al.Toward site-specific,homogeneous and highly stable fluorescent silver nanoclusters fabrication on triplex DNA scaffolds[J].Nucleic Acids Res.,2012,40(16):e122.
[12]Xu J,Wei C.The aptamer DNA-templated fluorescence silver nanoclusters:ATP detection and preliminary mechanism investigation[J].Biosens.Bioelectron.,2017(87):422-427.
[13]Thyrhaug E,Bogh SA,Carro-Temboury M R,et al.Ultrafast coherence transfer in DNA-templated silver nanoclusters[J].Nat.Commun.,2017(8):15577.
[14]Lee ST,Beaumont D,Su XD,et al.Formulation of DNA chimera templates:Effects on emission behavior of silver nanoclusters and sensing[J].Anal.Chim.Acta,2018(1010):62-68.
[15]Zheng C,Zheng X,Liu B,et al.One-pot synthesized DNA-templated Ag/Pt bimetallic nanoclusters as peroxidase mimics for colorimetric detection of thrombin[J].Chem.Commun.,2014,50(86):13103-13106.
[16]Yu CJ,Chen TH,Jiang JY,et al.Lysozyme-directed synthesis of platinum nanoclusters as a mimic oxidase[J].Nanoscale,2014(6):9618-9624.
[2]Gao LZ,Zhuang J,Nie L,et al.Intrinsic peroxidase-like activity of ferromagnetic nanoparticles[J].Nat.Nanotechnol.,2007,2(9):577-583.
[3]Li RM,Zhen MM,Guan MR,et al.A novel glucose colorimetric sensor based on intrinsic peroxidase-like activity of C60-carboxyfullerenes[J].Biosens.Bioelectron.,2013(47):502-507.
[4]Lan J,Xu W,Wan Q,et al.Colorimetric determination of sarcosine in urine samples of prostatic carcinoma by mimic enzyme palladium nanoparticles[J].Anal.Chim.Acta,2014,825(12):63-68.
[5]Dalui A,Pradhan B,Thupakula U,et al.Insight into the mechanism revealing the peroxidase mimetic catalytic activity of quaternary CuZnFeS nanocrystals:colorimetric biosensing of hydrogen peroxide and glucose[J].Nanoscale,2015(19):9062-9074.
[6]Tian,L,Qi JX,Oderinde O,et al.Planar intercalated copper(II)complex molecule as small molecule enzyme mimic combined with Fe3O4nanozyme for bienzyme synergistic catalysis applied to the microRNA biosensor[J].Biosens.Bioelectron.,2018(110):110-117.
[7]张雅云,钟炎妹,李顺兴,等.基于纳米材料及碳量子点对乙醇的电催化研究[J].闽南师范大学学报(自然科学版),2014,83(1):78-83.
[8]Fu XM,Liu ZJ,Cai SX,et al.Electrochemical aptasensor for the detection of vascular endothelial growth factor(VEGF)based on DNA-templated Ag/Pt bimetallic nanoclusters[J].Chin.Chem.Lett,27,2016:920-926.
[9]Feng J,Huang P,Wu FY.Gold-platinum bimetallic nanoclusters with enhanced peroxidase-like activity and their integrated agarose hydrogel-based sensing platform for the colorimetric analysis of glucose levels in serum[J].Analyst,2017,142(21):4106-4115.
[10]Song Q,Shi Y,He D,et al.Sequence dependent dsDNA-templated formation of fluorescent copper nanoparticles[J]Chemistry,2015,21(6):2417-2422.
[11]Feng L,Huang Z,Ren J,et al.Toward site-specific,homogeneous and highly stable fluorescent silver nanoclusters fabrication on triplex DNA scaffolds[J].Nucleic Acids Res.,2012,40(16):e122.
[12]Xu J,Wei C.The aptamer DNA-templated fluorescence silver nanoclusters:ATP detection and preliminary mechanism investigation[J].Biosens.Bioelectron.,2017(87):422-427.
[13]Thyrhaug E,Bogh SA,Carro-Temboury M R,et al.Ultrafast coherence transfer in DNA-templated silver nanoclusters[J].Nat.Commun.,2017(8):15577.
[14]Lee ST,Beaumont D,Su XD,et al.Formulation of DNA chimera templates:Effects on emission behavior of silver nanoclusters and sensing[J].Anal.Chim.Acta,2018(1010):62-68.
[15]Zheng C,Zheng X,Liu B,et al.One-pot synthesized DNA-templated Ag/Pt bimetallic nanoclusters as peroxidase mimics for colorimetric detection of thrombin[J].Chem.Commun.,2014,50(86):13103-13106.
[16]Yu CJ,Chen TH,Jiang JY,et al.Lysozyme-directed synthesis of platinum nanoclusters as a mimic oxidase[J].Nanoscale,2014(6):9618-9624.