正交试验优化健脾益肾丸的水提工艺
|
摘要
目的:优化健脾益肾丸的水提工艺。方法:以加水量、提取时间、提取次数为考察因素,以提取液中丹酚酸B含量和干膏量为综合评价指标,采用正交试验设计法优化健脾益肾丸的提取工艺。结果:最优提取工艺为提取2次,第1次加12倍水,第2次加8倍水,每次提取1 h。验证试验中综合评分平均值为95.5(RSD=0.52%,n=3)。结论:优化的水提工艺简便、可行,结果稳定,可用于健脾益肾丸的提取制备。
OBJECTIVE:To optimize the water extraction technology of Jianpi yishen pills.METHODS:Using water amount,extraction time and extraction times as factors,the content of salvianolic acid B and dry extract amount as index,orthogonal test was employed to optimize the extraction technology of Jianpi yishen pills.RESULTS:The optimal extraction technology was extracting twice,adding 12-fold water for the first time,8-fold water for the second time,extracting for 1 h each time.Average comprehensive score of validation test was 95.5(RSD=0.52%,n=3).CONCLUSIONS:The optimized technology is simple,suitable and stable,and can be used for the extraction and preparation of Jianpi yishen pills.
OBJECTIVE:To optimize the water extraction technology of Jianpi yishen pills.METHODS:Using water amount,extraction time and extraction times as factors,the content of salvianolic acid B and dry extract amount as index,orthogonal test was employed to optimize the extraction technology of Jianpi yishen pills.RESULTS:The optimal extraction technology was extracting twice,adding 12-fold water for the first time,8-fold water for the second time,extracting for 1 h each time.Average comprehensive score of validation test was 95.5(RSD=0.52%,n=3).CONCLUSIONS:The optimized technology is simple,suitable and stable,and can be used for the extraction and preparation of Jianpi yishen pills.
引文
[1]易无庸,杨曙东,李顺民,等.健脾益肾方治疗维持性血透患者营养不良的临床研究[J].新中医,2004,36(11):16.
[2]李顺民,杨曙东.健脾益肾方治疗脾肾气虚型慢性肾功能衰竭46例疗效观察[J].新中医,2005,37(10):36.
[3]黄桢.黄芪多糖的药理研究进展[J].中国临床药学杂志,2002,11(5):315.
[4]刘雄,李成明,高建德,等.肉苁蓉的研究进展[J].中国中医药科技,2013,20(5):575.
[5]孙仁弟,徐向阳,宋燕青.丹酚酸B的药理研究进展[J].药物流行病学杂志,2012,20(9):458.
[6]国家药典委员会.中华人民共和国药典:四部[S].2015年版.北京:中国医药科技出版社,2015:103.
[7]牟婵,周若鹏,赖滢滢.正交试验法优选复方淫羊藿咀嚼片的提取工艺[J].中国药房,2015,26(28):3 986.
[8]易铁钢,易无庸,周昕.健脾益肾丸对维持性血透患者微炎症状态的影响[J].中国中医药科技,2011,18(5):377.
[9]刘静.正交设计法在药物制备工艺研究中的应用[J].齐鲁药事,2011,30(11):668.
[10]赵燕,李秋芬,张忠亮,等.参芪麝蓉丸质量评价[J].中草药,2014,45(7):942.
[2]李顺民,杨曙东.健脾益肾方治疗脾肾气虚型慢性肾功能衰竭46例疗效观察[J].新中医,2005,37(10):36.
[3]黄桢.黄芪多糖的药理研究进展[J].中国临床药学杂志,2002,11(5):315.
[4]刘雄,李成明,高建德,等.肉苁蓉的研究进展[J].中国中医药科技,2013,20(5):575.
[5]孙仁弟,徐向阳,宋燕青.丹酚酸B的药理研究进展[J].药物流行病学杂志,2012,20(9):458.
[6]国家药典委员会.中华人民共和国药典:四部[S].2015年版.北京:中国医药科技出版社,2015:103.
[7]牟婵,周若鹏,赖滢滢.正交试验法优选复方淫羊藿咀嚼片的提取工艺[J].中国药房,2015,26(28):3 986.
[8]易铁钢,易无庸,周昕.健脾益肾丸对维持性血透患者微炎症状态的影响[J].中国中医药科技,2011,18(5):377.
[9]刘静.正交设计法在药物制备工艺研究中的应用[J].齐鲁药事,2011,30(11):668.
[10]赵燕,李秋芬,张忠亮,等.参芪麝蓉丸质量评价[J].中草药,2014,45(7):942.