正交试验优化黄芪消渴汤水提工艺
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摘要
目的研究黄芪消渴汤的最佳提取工艺。方法采用正交试验设计,以黄芪甲苷含量及干膏量为指标,考察水的用量、煎煮时间及煎煮次数3个因素对黄芪消渴汤提取效果的影响,优选提取工艺。结果黄芪消渴汤的最佳工艺条件为煎煮1 h,煎煮3次,加6倍量水。结论该提取工艺合理可行,能较好的保证制剂质量。
OBJECTIVE To optimize the extraction techniques for the compound of Huangqixiaoke decoction. METHODS With the contents of astragaloside Ⅳand the dry extract amount as the observation index,and using the amount of water,the boiling time,boiling frequency as factors,water extraction techniques was optimized. RESULTS The optimized extracting condition of Huangqixiaoke decoction was as follows: adding 6-fold water to decoct for 3 times,1 h for each time. CONCLUSION This extraction process is reasonable and practical,and can well guarantee its quality.
OBJECTIVE To optimize the extraction techniques for the compound of Huangqixiaoke decoction. METHODS With the contents of astragaloside Ⅳand the dry extract amount as the observation index,and using the amount of water,the boiling time,boiling frequency as factors,water extraction techniques was optimized. RESULTS The optimized extracting condition of Huangqixiaoke decoction was as follows: adding 6-fold water to decoct for 3 times,1 h for each time. CONCLUSION This extraction process is reasonable and practical,and can well guarantee its quality.
引文
[1]黄寅銮,曾纪斌,许希雪,等.黄芪消渴方联合西药治疗糖尿病肾病的临床观察[J].中国名族明间医药,2017,26(1):81-82.
[2]曾纪斌,潘艳,黄寅銮,等.黄芪消渴方对气阴两虚型2型糖尿病患者中医症状改善的影响[J].新中医,2014,46(4):146-148.
[3]魏敏,江雪华.正交设计法优选黄芪提取工艺研究[J].基层中药杂志,2000,14(6):123-125.
[4]俞冰,刘志承,李曙光,等.正交试验优化固本祛风颗粒提取工艺[J].中国药业,2013,22(14):79-82.
[5]周芹.黄芪甲苷抗凋亡作用机制的研究进展[J].中国现代应用药学,2017,34(5):783-788.
[6]江清林,姜吉文,吕学诜,等.黄芪甲苷对类胰升血糖素肽-1作用的研究[J].中国老年学杂志,2003,23(1):52.
[7]国家药典委员会.中华人民共和国药典[M].北京:中国医药科技出版社,2015.
[8]丁水平,马宝瑕,张锐,等.黄芪甲苷检测在黄芪及其制剂质控中的应用[J].中国药师,2001,4(5):360-362.
[9]王春怡,李卫民.HPLC-ELSD法测定不同产地黄芪中黄芪甲苷的含量[J].辽宁中医药大学学报,2008,10(10):144-145.
[10]罗雨,李文龙,黄文华,等.HPLC-UV-ELSD同时测定黄芪水提液与含醇提取物中6种成分[J].中国中药杂志,2016,41(5):850-858.
[2]曾纪斌,潘艳,黄寅銮,等.黄芪消渴方对气阴两虚型2型糖尿病患者中医症状改善的影响[J].新中医,2014,46(4):146-148.
[3]魏敏,江雪华.正交设计法优选黄芪提取工艺研究[J].基层中药杂志,2000,14(6):123-125.
[4]俞冰,刘志承,李曙光,等.正交试验优化固本祛风颗粒提取工艺[J].中国药业,2013,22(14):79-82.
[5]周芹.黄芪甲苷抗凋亡作用机制的研究进展[J].中国现代应用药学,2017,34(5):783-788.
[6]江清林,姜吉文,吕学诜,等.黄芪甲苷对类胰升血糖素肽-1作用的研究[J].中国老年学杂志,2003,23(1):52.
[7]国家药典委员会.中华人民共和国药典[M].北京:中国医药科技出版社,2015.
[8]丁水平,马宝瑕,张锐,等.黄芪甲苷检测在黄芪及其制剂质控中的应用[J].中国药师,2001,4(5):360-362.
[9]王春怡,李卫民.HPLC-ELSD法测定不同产地黄芪中黄芪甲苷的含量[J].辽宁中医药大学学报,2008,10(10):144-145.
[10]罗雨,李文龙,黄文华,等.HPLC-UV-ELSD同时测定黄芪水提液与含醇提取物中6种成分[J].中国中药杂志,2016,41(5):850-858.