‘保罗兰’睡莲花粉离体萌发及花粉管生长的研究
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摘要
【目的】研究不同培养基组分、不同培养时间对‘保罗兰’睡莲花粉萌发和花粉管生长的影响,为睡莲杂交育种提供科学的依据。【方法】采用花粉离体萌发法,用显微镜观察花粉离体萌发情况。【结果】20和40 mg/L的硼酸(H_3BO_3)处理均能促进花粉萌发,适宜浓度的蔗糖和低浓度的Ca~(2+)处理能显著提高花粉的萌发率;睡莲花粉在培养的4 h之内,花粉萌发率上升较快,4～6 h萌发率趋于稳定。花粉管生长在前2 h生长较快,2～6 h生长趋于稳定。【结论】10%蔗糖+20 mg/L或40 mg/L H_3BO_3+20 mg/L氯化钙(CaCl_2)是‘保罗兰’睡莲花粉最适萌发培养基组分,最佳镜检时间为4 h。
【Objective】To provide scientific basis for hybrid breeding of water lily, the effects of different medium components and culture time on pollen germination and tube growth of Nymphaea ‘Paul Stetson' were studied.【Method】The pollen germination in vitro was observed by microscope.【Result】H_3BO_3 of 20 and 40 mg/L could promote pollen germination, Sucrose of suitable concentration and Ca~(2+) of low concentration could significantly increase the germination rate of pollen. The pollen germination rate increased rapidly in the pre 4 hours, and the germination rate of 4 hours to 6 hours tended to be stable. Pollen tubes grew faster in the pre 2 hours and tended to be stable from 2 hours to 6 hours.【Conclusion】10 % sucrose+20 mg/L or 40 mg/L H_3BO_3+20 mg/L CaCl_2 was the optimum medium for the germination of Nymphaea ‘Paul Stetson' pollen, and the optimum microscopic examination time is 4 hours.
【Objective】To provide scientific basis for hybrid breeding of water lily, the effects of different medium components and culture time on pollen germination and tube growth of Nymphaea ‘Paul Stetson' were studied.【Method】The pollen germination in vitro was observed by microscope.【Result】H_3BO_3 of 20 and 40 mg/L could promote pollen germination, Sucrose of suitable concentration and Ca~(2+) of low concentration could significantly increase the germination rate of pollen. The pollen germination rate increased rapidly in the pre 4 hours, and the germination rate of 4 hours to 6 hours tended to be stable. Pollen tubes grew faster in the pre 2 hours and tended to be stable from 2 hours to 6 hours.【Conclusion】10 % sucrose+20 mg/L or 40 mg/L H_3BO_3+20 mg/L CaCl_2 was the optimum medium for the germination of Nymphaea ‘Paul Stetson' pollen, and the optimum microscopic examination time is 4 hours.
引文
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[18]张瑞,李洋,梁有旺,等.薄壳山核桃花粉离体萌发和花粉管生长特性研究[J].西北植物学报,2013,33(9):1916-1922.
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[21]de Graaf B H J,Derksen J W M,Mariani C.Pollen and pistil in the progamic phase[J].Sexual Plant Reproduction,2001,14(1-2):41-55.
[22]李千惠,姜文龙,徐同乐,等.日本海棠花粉最佳离体萌发培养基筛选及萌发过程研究[J].经济林研究,2018(2).
[23]汤红明,徐冬青,徐根娣,等.植物花粉萌发的研究进展[J].安徽农业科学,2006,34(24):6436-6438.
[24]Bednarska E.The effect of exogenous Ca2+ ions on pollen grain germination and pollen tube growth:Investigations with Ca2+ together with verapamil,La3+ and ruthenium red[J].Sexual Plant Reproduction,1989,2(1):53-58.
[25]白天,解玮佳,李世峰,等.糖、硼、钙对大喇叭杜鹃四合花粉萌发的影响[J].上海交通大学学报(农业科学版),2013,31(6):48-54.
[26]龚明,曹宗巽.钙和钙调素对花粉萌发和花粉管生长的调控[J].植物生理学报,1995(5):321-328.
[27]郝怀庆,王钦丽,陈艳梅.花粉管细胞壁结构及胞质运动[J].植物学报,2003,20(3):270-279.
[28]游韩莉,贾婷婷,田菊,等.杜仲花粉离体萌发特征及花粉管微丝骨架分布[J].西北植物学报,2014,34(3):495-501.
[29]王晓华,郝怀庆,王钦丽,等.花粉管细胞结构与生长机制研究进展[J].植物学报,2007,24(3):340-354.
[2]廖卫伟,杨志娟,朱天龙,等.睡莲的植物学性状及其分类[J].现代农业科技,2016(24):148,154.
[3]余翠薇,陈煜初,余东北,等.睡莲5个亚属花、叶、块茎和基因组大小比较[J].浙江农业科学,2017,58(8):1353-1356,1361.
[4]李子俊.我国首批跨亚属耐寒睡莲育成[J].中国花卉盆景,2013(10):4-7.
[5]刘义满,Slearmlarp Wasuwat,柯卫东,等.泰国睡莲考察报告[J].中国园艺文摘,2009(1).
[6]黄国振.睡莲[M].北京:中国林业出版社,2009:23-25,45,134-182.
[7]R E Preston.The Intrafloral phenology of Streptanthus tortuosus(Brassicaceae)[J].American Journal of Botany,1991,78(8):1044-1053.
[8]李佛莲,徐海燕,龚洵,等.山玉兰花粉离体萌发和花粉管生长特性的研究[J].广西植物,2017,37(4):478-484.
[9]周莉花,郝日明,赵宏波.蜡梅花粉活力检测方法筛选及保存时间观察[J].浙江农林大学学报,2006,23(3):37-41.
[10]姜雪婷,杜玉虎,张绍铃,等.梨43个品种花粉生活力及4种测定方法的比较[J].果树学报,2006,23(2):26-29.
[11]于艳杰,吴李君,吴跃进.陆地棉花粉粒萌发和花粉管生长特性[J].自然科学进展,2007,17(9):1299-1303.
[12]赵其龙.硼酸和蔗糖对‘柔毛齿叶’睡莲花粉萌发的影响[J].亚热带植物科学,2012(4):45-47.
[13]张亚栋,郝青,吕少杰,等.青岛耐寒睡莲花粉生活力研究[A].见:张启翔主编.中国观赏园艺研究进展2015[C].厦门:中国林业出版社,2015:57-61.
[14]杨梦飞,李怡鹏,楼宇涛,等.培养液组分及培养时间对睡莲花粉萌发和花粉管生长的影响[J].浙江农业科学,2016,57(10):1689-1691.
[15]王钦丽,卢龙斗,吴小琴,等.花粉的保存及其生活力测定[J].植物学报,2002,19(3):365-373.
[16]孙春丽,潘延云.拟南芥花粉活力的测定及其在花粉发育研究中的应用[J].植物学报,2008,25(3):268-275.
[17]杨姗,张家瑞,秦垚,等.“素心”蜡梅花粉生活力及贮藏力的研究[J].重庆师范大学学报(自然科学版),2016(2):134-137.
[18]张瑞,李洋,梁有旺,等.薄壳山核桃花粉离体萌发和花粉管生长特性研究[J].西北植物学报,2013,33(9):1916-1922.
[19]James L B,Beyoung H K.The essential role of calcium ion in pollen germination and pollen tube growth[J].American Journal of Botany,1963,50(9):859-865.
[20]邓衍明,叶晓青,梁丽建.茉莉花粉离体培养萌发及花粉管生长特性研究[J].华北农学报,2014,29(5):107-113.
[21]de Graaf B H J,Derksen J W M,Mariani C.Pollen and pistil in the progamic phase[J].Sexual Plant Reproduction,2001,14(1-2):41-55.
[22]李千惠,姜文龙,徐同乐,等.日本海棠花粉最佳离体萌发培养基筛选及萌发过程研究[J].经济林研究,2018(2).
[23]汤红明,徐冬青,徐根娣,等.植物花粉萌发的研究进展[J].安徽农业科学,2006,34(24):6436-6438.
[24]Bednarska E.The effect of exogenous Ca2+ ions on pollen grain germination and pollen tube growth:Investigations with Ca2+ together with verapamil,La3+ and ruthenium red[J].Sexual Plant Reproduction,1989,2(1):53-58.
[25]白天,解玮佳,李世峰,等.糖、硼、钙对大喇叭杜鹃四合花粉萌发的影响[J].上海交通大学学报(农业科学版),2013,31(6):48-54.
[26]龚明,曹宗巽.钙和钙调素对花粉萌发和花粉管生长的调控[J].植物生理学报,1995(5):321-328.
[27]郝怀庆,王钦丽,陈艳梅.花粉管细胞壁结构及胞质运动[J].植物学报,2003,20(3):270-279.
[28]游韩莉,贾婷婷,田菊,等.杜仲花粉离体萌发特征及花粉管微丝骨架分布[J].西北植物学报,2014,34(3):495-501.
[29]王晓华,郝怀庆,王钦丽,等.花粉管细胞结构与生长机制研究进展[J].植物学报,2007,24(3):340-354.