免疫球蛋白G N-糖基化与糖尿病视网膜病变的相关性研究
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摘要
目的探讨糖尿病视网膜病变(DR)与免疫球蛋白G (IgG)N-糖基化的相关性,为疾病的发生发展提供依据。方法将纳入的2型糖尿病患者以是否患有DR分为病例组和对照组,比较两组IgG N-糖基化水平,通过二分类logistic模型校正其他协变量。结果直接测量的糖基峰中,GP4、GP6、GP17和GP19在病例组水平较高,而GP15、GP16、GP18和GP21在对照组含量较高(P<0.05);在校正年龄、性别等其他协变量后,代表唾液酸化的10种衍生结构差异均有统计学意义(P<0.05)。结论 IgG N-糖基化的唾液酸化与DR的发生发展有相关性。
Objective To explore the association between diabetic retinopathy(DR)and immunoglobulin G(IgG)N-glycosylation,and to provide basis for disease control. Methods Type 2 diabetes mellitus(T2 DM)patients were divided into case group(with DR)and control group(without DR).The levels of IgG N-glycosylation were compared between two groups and the other covariates were adjusted by dichotomous logistic model. Results Among the directed measured glycans,the levels of GP4,GP6,GP17 and GP19 were higher in case group,while the levels of GP15,GP16,GP18 and GP21 were higher in control group(P<0.05).After adjusting for other covariates by age and gender,the differences of ten derived glycans representing sialylation were significant(P<0.05). Conclusions There is a positive correlation between IgG N-glycosylated sialylation and the development of DR.
Objective To explore the association between diabetic retinopathy(DR)and immunoglobulin G(IgG)N-glycosylation,and to provide basis for disease control. Methods Type 2 diabetes mellitus(T2 DM)patients were divided into case group(with DR)and control group(without DR).The levels of IgG N-glycosylation were compared between two groups and the other covariates were adjusted by dichotomous logistic model. Results Among the directed measured glycans,the levels of GP4,GP6,GP17 and GP19 were higher in case group,while the levels of GP15,GP16,GP18 and GP21 were higher in control group(P<0.05).After adjusting for other covariates by age and gender,the differences of ten derived glycans representing sialylation were significant(P<0.05). Conclusions There is a positive correlation between IgG N-glycosylated sialylation and the development of DR.
引文
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[15]张婷婷,陈忠平.炎症相关疾病—糖尿病视网膜病变[J].中国医师杂志,2017,19(10):1598-1600.
[2] Noda K,Ishida S.Role of chronic inflammation in diabetic retinopathy[J].Inflamm Regen,2013,33(5):230-237.
[3] Afzal N,Zaman S,Shahzad F,et al.Immune mechanisms in type-2diabetic retinopathy[J].J Pak Med Assoc,2015,65(2):159-163.
[4] Sebastian A,Alzain MA,Asweto CO,et al.Glycan biomarkers for rheumatoid arthritis and its remission status in Han Chinese patients[J].OMICS,2016,20(6):343-351.
[5] Akmaˇcic IT,Ventham NT,Theodoratou E,et al.Inflammatory bowel disease associates with proinflammatory potential of the immunoglobulin G glycome[J].Inflamm Bowel Dis,2015,21(6):1237-1247.
[6] Sj9wall C,Zapf J,von LS,et al.Altered glycosylation of complexed native IgG molecules is associated with disease activity of systemic lupus erythematosus[J].Lupus,2015,24(6):569-581.
[7] Lemmers RF,Vilaj M,Urda D,et al.IgG glycan patterns are associated with type 2diabetes in independent European populations[J].Biochim Biophys Acta,2017,1861(9):2240-2249.
[8] The Royal College of Ophthalmologist.The royal college of ophthalmologists diabetic retinopathy guidelines[EB/OL].[2018-04-06].https://www.worldcat.org/identities/lccnno98104720/.
[9]朱秋荣,骆田斌,徐惠庆,等.嘉兴市秀洲区2009-2014年糖尿病流行病学调查研究[J].中国预防医学杂志,2017,18(8):613-617.
[10] Quast I,Keller CW,Maurer MA,et al.Sialylation of IgG Fc domain impairs complement-dependent cytotoxicity[J].J Clin Invest,2015,125(11):4160-4170.
[11] Wong AH,Fukami Y,Sudo M,et al.Sialylated IgG-Fc:a novel biomarker of chronic inflammatory demyelinating polyneuropathy[J].J Neurol Neuro Psychiatry,2016,87(3):275-279.
[12] Vuˇckovic F,Kri2tic J,Gudelj I,et al.Association of systemic lupus erythematosus with decreased immunosuppressive potential of the IgG glycome[J].Arthritis Rheumatol,2015,67(11):2978-2989.
[13] Tanigaki K,Sacharidou A,Peng J,et al.Hyposialylated IgG activates endothelial IgG receptor FcγRIIB to promote obesityinduced insulin resistance[J].J Clin Invest,2018,128(1):309-322.
[14] Yu Y,Chen H,Su SB.Neuroinflammatory responses in diabetic retinopathy[J].J Neuroinflammation,2015,12:141.
[15]张婷婷,陈忠平.炎症相关疾病—糖尿病视网膜病变[J].中国医师杂志,2017,19(10):1598-1600.