液相色谱-大气压化学电离质谱分析鉴别地沟油中的氧化成分

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英文篇名：Determination of Oxidation Products of Glycerides in Hogwash Oil Using High Performance Liquid Chromatography-Atmospheric Pressure Chemical Ionization Mass Spectrometry 作者：王世成 ; 范津杉 ; 王颜红 ; 李国琛 ; 张红 ; 王莹 英文作者：WANG Shi-Cheng;FAN Jin-Shan;WANG Yan-Hong;LI Guo-Chen;ZHANG Hong;WANG Ying;Shenyang Key Laboratory of Detection and Control Technology of Food Safety,Institute of Applied Ecology,Chinese Academy of Sciences; 关键词：高效液相色谱-质谱 ; 地沟油 ; 甘油三酯环氧化物 ; 偏最小二乘法判别分析(PLS-DA) 英文关键词：High performance liquid chromatography-mass spectrometry;;Hogwash oil;;Epoxides of triacylglycerols;;Partial least square discriminate analysis 中文刊名：FXHX 英文刊名：Chinese Journal of Analytical Chemistry 机构：中国科学院沈阳应用生态研究所沈阳市食品安全检测与控制技术重点实验室; 出版日期：2014-05-09 08:54 出版单位：分析化学 年：2014 期：v.42 基金：辽宁省科技计划项目(No.2010415015);; 沈阳市科技计划项目(No.F12-162-9-00)资助 语种：中文; 页：FXHX201405026 页数：6 CN：05 ISSN：22-1125/O6 分类号：129-134

摘要

采用液相色谱-大气压化学电离质谱(HPLC-APCI-MS)方法,分析了食用油脂和地沟油的甘油三酯(TAG)组成。采用C18色谱柱,乙腈-丙酮为流动相,梯度洗脱,APCI正离子模式检测。应用本方法检测了15个地沟油和11个食用油样品,结果表明,地沟油中存在三亚油酸甘油酯(LLL)、二亚油酸单油酸甘油酯(LOL)、二亚油酸单亚麻酸甘油酯(LLnL),二油酸单亚油酸甘油酯(OOL)等甘油三酯的5种亚油酰基氧化产物。采用偏最小二乘法判别分析(PLS-DA)化学计量学方法,分析建立了地沟油和正常食用油的判别模型,实现了正常食用油和地沟油区分,模型正确判别率96.2%。在判别模型中,含有亚油酸环氧化物的TAG分子对判别贡献率较大,该类化合物可作为地沟油区别于正常油脂的标志成分。
        Triacylglycerols( TAGs) in edible oils( soybean,peanut,sesame) and hogwash oil were analyzed by HPLC-MS with atmospheric pressure chemical ionization( APCI). The gradient elution chromatographic separation was performed on a C18column using mobile phase of acetonitrile-acetone,and TAGs were monitored by APCI-MS in positive mode. 15 hogwash oils and 11 normal edible oils were analysed with the method. Five linoleoyl epoxides of trilinolein( LLL),dilinoleoyl-oleoyl glycerol( LOL),dilinoleoyl-linolenoyl glycerol( LLnL),dioleoyl-linoleoyl glycerol( OOL) in hogwash oil were detected. The discriminantion model established with partial least square discriminate analysis( PLS-DA),could distinguish the normal edible oil and the hogwash oil with an accuracy of 96. 2%. The linoleoyl epoxides of TAGs made the major distinguishing contribution for the model,and were proved as the mark components in hogwash oil different from the normal edible oil.

引文

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