静张应力对大鼠髁突软骨细胞增殖效应调节研究
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摘要
目的:就静张应力对大鼠髁突软骨细胞增殖效应调节进行研究。方法:对SD大鼠下颌髁突软骨细胞予以培养,分别在小牛血清浓度为15%、5%的DMEM培养基中培养第4代髁突软骨细胞0h、6h、12h后,对其DNA含量予以测定;分别在静张应力为10k Pa、5k Pa的DMEM培养基中培养第4代髁突软骨细胞0h、2h、4h、6h、8h、10h、12h后,对其DNA含量予以测定。结果:当静张应力为15k Pa时,随着培养时相的延长,大鼠髁突软骨细胞的PI指数也同样呈现出"先逐步上升、后又下降"的趋势,但是下降的起始时间要比静张应力为5k Pa时更早,在4~12h时的PI指数与对照组存在着较为明显的差异,具有统计学意义(P<0.05)。结论:髁突软骨细胞的增殖活性能够被静张应力所调节,值得推广应用。
Objective: To study the effects of static tension- stress on proliferation of MCC of rats. Methods:Mandibular condylar chondrocyte cells of SD rats were cultivated. The fourth- passage chondrocytes in the DMEM medium which calf serum concentration was 15% 、5%,respectively for 0h、6h、12h,and then the content of DNA was tested. Results: When the static tension- stress was 15 k Pa,with the extension of incubation time,the PI index of MCC also presented "upgrade firstly and then dropped",but the start time of falling was earlier than the time of static tension- stress 5k Pa. PI index of 4 ~ 12 h existed significant difference compared with the control group with significant statistics( P < 0. 05). Conclusion: Proliferation activity of MCC can be regulated by static tension- stress,which is worthy of popularizing and applying.
Objective: To study the effects of static tension- stress on proliferation of MCC of rats. Methods:Mandibular condylar chondrocyte cells of SD rats were cultivated. The fourth- passage chondrocytes in the DMEM medium which calf serum concentration was 15% 、5%,respectively for 0h、6h、12h,and then the content of DNA was tested. Results: When the static tension- stress was 15 k Pa,with the extension of incubation time,the PI index of MCC also presented "upgrade firstly and then dropped",but the start time of falling was earlier than the time of static tension- stress 5k Pa. PI index of 4 ~ 12 h existed significant difference compared with the control group with significant statistics( P < 0. 05). Conclusion: Proliferation activity of MCC can be regulated by static tension- stress,which is worthy of popularizing and applying.
引文
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[2]饶跃,罗颂椒,王大章,等.功能矫形前伸下颌后对大鼠髁突软骨DNA合成影响的研究[J].华西口腔医学杂志,1992,10(3):214-216
[3]周征,罗颂椒.大鼠下颌髁突软骨中IGF_1及其基因的差异性表达[J].华西口腔医学杂志,1998,16(2):164-166
[4]杨红岩,刘继光,王本才,等.咬合创伤兔颞下颌关节的组织学变化的实验研究[J].黑龙江医药科学,2007,25(3):42-43
[5]胡春江,徐宏光.细胞培养方式与力学刺激[J].黑龙江医药科学,2010,33(6):19-21