槐耳清膏对人肺腺癌A549/DDP细胞增殖的影响及顺铂耐药逆转作用
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摘要
目的:探讨槐耳清膏对人肺腺癌A549细胞和A549/DDP细胞增殖的抑制影响及其顺铂耐药细胞A549/DDP的逆转作用。方法:以体外培养的人肺腺癌A549及A549/DDP细胞为实验模型,采用MTT法测定不同浓度槐耳清膏对A549细胞及A549/DDP细胞的增殖抑制率,并运用流式细胞术检测顺铂联合槐耳清膏对A549/DDP细胞的变化,以及增敏作用效应。结果:槐耳清膏对A549细胞及A549/DDP细胞的增殖有明显抑制作用,随着药物浓度的增加,其抑制率逐渐增加,具有明显的量-效关系(P<0.05)。顺铂40μmol/L作用于A549及A549/DDP细胞48 h后,其凋亡率分别为(27.61±3.45)%和(17.93±1.73)%,两组间有统计学差异(P<0.01)。槐耳清膏1 mg/m L作用于人肺腺癌A549和A549/DDP细胞40 h后,再以相同浓度顺铂诱导,A549细胞凋亡率为(32.35±2.68)%,A549/DDP细胞凋亡率为(30.01±3.65)%,两组间无统计学意义,但与单独DDP作用相比,差异具有显著意义(P<0.01)。结论:槐耳清膏能显著抑制人肺腺癌A549及A549/DDP细胞的增殖,诱导细胞凋亡,还可以增加A549/DDP细胞对顺铂的敏感性。
Objective: To observe the effect of Huaier on proliferation and the reversal effect of Huaier on cisplatin-resistant human lung adenocarcinoma cell line A549 / DDP. Methods: A model of cell line A549 and A549 / DDP of cisplatin resistance in vitro was built. MTT was used to detect proliferation and flow cytometry( FCM) used to analyze the interaction of cisplatin and to A549 / DDP cell and sensitization to cisplatin resistance. Results: Huaier inhibited the proliferation of A549 and A549 / DDP cells and the inhibition rate increased with the increase of the concentration of Huaier. It was time- and dose- dependent. The inhibition rate of A549 and A549 / DDP after 48 hours was different from that in the group of 40 μmol / L cisplatin[( 27. 61 ± 3. 45) % vs( 17. 93 ± 1. 73) % ]( P < 0. 05). After 1 mg / m L Huaier intervening for 40 hours later,the apoptosis rate of A549 / DDP induced by cisplatin and Huaier was( 30. 01 ± 3. 65) %,not significantly different from that of A549( 32. 35 ± 2. 68) %( P > 0. 05),but was significantly different from the group of cisplatin only( P < 0. 01). Conclusion: Huaier shows cell proliferation inhibitory and apoptosis inducing effects on human lung adenocarcinoma A549 and A549 / DDP cells. Huaier improves the sensibility of A549 / DDP cells to cisplatin.
Objective: To observe the effect of Huaier on proliferation and the reversal effect of Huaier on cisplatin-resistant human lung adenocarcinoma cell line A549 / DDP. Methods: A model of cell line A549 and A549 / DDP of cisplatin resistance in vitro was built. MTT was used to detect proliferation and flow cytometry( FCM) used to analyze the interaction of cisplatin and to A549 / DDP cell and sensitization to cisplatin resistance. Results: Huaier inhibited the proliferation of A549 and A549 / DDP cells and the inhibition rate increased with the increase of the concentration of Huaier. It was time- and dose- dependent. The inhibition rate of A549 and A549 / DDP after 48 hours was different from that in the group of 40 μmol / L cisplatin[( 27. 61 ± 3. 45) % vs( 17. 93 ± 1. 73) % ]( P < 0. 05). After 1 mg / m L Huaier intervening for 40 hours later,the apoptosis rate of A549 / DDP induced by cisplatin and Huaier was( 30. 01 ± 3. 65) %,not significantly different from that of A549( 32. 35 ± 2. 68) %( P > 0. 05),but was significantly different from the group of cisplatin only( P < 0. 01). Conclusion: Huaier shows cell proliferation inhibitory and apoptosis inducing effects on human lung adenocarcinoma A549 and A549 / DDP cells. Huaier improves the sensibility of A549 / DDP cells to cisplatin.
引文
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[3]胡保全,唐鹏,齐晓伟,等.槐耳清膏对乳腺癌细胞系SUM-159细胞干性特征的影响[J].第三军医大学学报,2013,35(11):1107-1110.
[4]海艳洁,郑宇,庄亚严,等.槐耳颗粒联合化疗对晚期大肠癌的初步临床研究[J].药物流行病学杂志,2012,21(2):53-55.
[5]周平,雷秋模,曹亚丽,等.槐耳颗粒治疗老年乳腺癌的临床疗效[J].中国老年学杂志,2012,9(32):3892-3893.
[6]Spiro SG,Tanner NT,Silvestri GA,et al.Lung cancer:Progress in diagnosis,staging and therapy[J].Respirology,2010,15(1):44-50.
[7]Mingqian Lu,Qingzhi Kong,Xinhua Xu,et al.Pectolinarigenin,a Flavonoid Compound from Cirsium Japonicum with Potential Anti-proliferation Activity in MCF-7 Breast Cancer Cell[J].Trop J Pharm Res,2014,13(2):225-228.
[8]Zhang N,Kong X,Yan S,et al.Huaier aqueous extract inhibits proliferation of breast cancer cells by inducing apoptosis[J].Cancer Sci,2010,101(11):2375-2383.
[9]Wu T,Chen W,Liu S,et al.Huaier suppresses proliferation and induces apoptosis in human pulmonary cancer cells via upregulation of miR-26b-5p[J].Cancer Sci,2014,588(12):2107-2114.
[10]喻喆,吴涛,张阳.槐耳清膏体外逆转人肝癌耐药细胞BEL-7402/5-Fu多药耐药性[J].肿瘤学杂志,2013,19(6):443-447.