槐耳清膏对人乳腺癌MCF-7及MDA-MB-231细胞增殖的影响
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摘要
目的:研究槐耳清膏对人乳腺癌MCF-7及MDA-MB-231细胞增殖的影响。方法:配制不同浓度的槐耳清膏(3、6、9 mg·ml~(-1))作用于MCF-7及MDA-MB-231细胞48 h后,MTT比色法测定槐耳清膏各浓度组对MCF-7及MDA-MB-231细胞的增殖抑制率。结果:槐耳清膏作用于乳腺癌MCF-7细胞48 h后,与阴性对照组比较,各浓度组的OD值逐渐下降,在槐耳清膏浓度为3、6、9 mg·ml~(-1)时其细胞抑制率分别为(17. 4±1. 4)%、(58. 6±3. 7)%、(76. 1±2. 9)%,差异具有统计学意义(P <0. 05)。槐耳清膏作用于乳腺癌MDAMB-231细胞48 h后,与阴性对照组比较,各浓度组的OD值变化不大,在槐耳清膏浓度为3、6、9 mg·ml~(-1)时其细胞抑制率分别为(10. 3±1. 7)%、(15. 1±2. 6)%、(17. 8±3. 9)%,差异无统计学意义(P> 0. 05)。结论:槐耳清膏对人乳腺癌MCF-7细胞的增殖具有明显的抑制作用,随着药物浓度的增加抑制作用更加明显。槐耳清膏对乳腺癌MDA-MB-231细胞无明显的抑制增殖作用。
Objective: To investigate the effects of Huaier on proliferation of human breast cancer MCF-7 and MDA-MB-231 cells. Methods: MCF-7 and MDA-MB-231 cells at the logarithmic phase were treated with 3,6 and9 mg·ml~(-1) Huaier cream for 48 h,respectively. MTT was used to determine OD value,and the inhibitory rate was calculated. Results: Compared with the negative control group,OD values in MCF-7 cells of each concentration group was gradually decreased,the inhibitory rates of MCF-7 cells were( 17. 4 ± 1. 4) %,( 58. 6 ± 3. 7) % and( 76. 1 ± 2. 9) %,when the concentrations of Huaier cream were 3,6,and 9 mg·ml~(-1),respectively,with statistically significant difference( P < 0. 05). Compared with the negative control group,OD values were not obviously changed in human breast cancer MDA-MB-231 cells after treatment with Huaier cream for 48 h and the inhibitory rates of MDA-MB-231 cells were( 10. 3 ± 1. 7) %,( 15. 1 ± 2. 6) % and( 17. 8 ± 3. 9) %,when the concentrations of Huaier cream were 3,6,and 9 mg·ml~(-1),respectively,and there was no statistically significant difference( P >0. 05). Conclusion: Huaier cream can obvious inhibit the proliferation of human breast cancer line MCF-7,the inhibitory role is enhanced by increasing the drug's concentration. While it does not inhibit the proliferation of human breast cancer cell line MDA-MB-231 obviously.
Objective: To investigate the effects of Huaier on proliferation of human breast cancer MCF-7 and MDA-MB-231 cells. Methods: MCF-7 and MDA-MB-231 cells at the logarithmic phase were treated with 3,6 and9 mg·ml~(-1) Huaier cream for 48 h,respectively. MTT was used to determine OD value,and the inhibitory rate was calculated. Results: Compared with the negative control group,OD values in MCF-7 cells of each concentration group was gradually decreased,the inhibitory rates of MCF-7 cells were( 17. 4 ± 1. 4) %,( 58. 6 ± 3. 7) % and( 76. 1 ± 2. 9) %,when the concentrations of Huaier cream were 3,6,and 9 mg·ml~(-1),respectively,with statistically significant difference( P < 0. 05). Compared with the negative control group,OD values were not obviously changed in human breast cancer MDA-MB-231 cells after treatment with Huaier cream for 48 h and the inhibitory rates of MDA-MB-231 cells were( 10. 3 ± 1. 7) %,( 15. 1 ± 2. 6) % and( 17. 8 ± 3. 9) %,when the concentrations of Huaier cream were 3,6,and 9 mg·ml~(-1),respectively,and there was no statistically significant difference( P >0. 05). Conclusion: Huaier cream can obvious inhibit the proliferation of human breast cancer line MCF-7,the inhibitory role is enhanced by increasing the drug's concentration. While it does not inhibit the proliferation of human breast cancer cell line MDA-MB-231 obviously.
引文
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[18]张芷旋,范羽,周清华,等.槐耳清膏对人高转移大细胞肺癌细胞L9981血管生成相关基因表达的影响[J].中国肺癌杂志,2006,9(2):137-142.
[19]JIN F P,ZHANG M. Progress of experimental researches on Chinese herbal compounds for inducing tumor cell apoptosis[J]. Chin J Integr Med,2010,16(6):565-571.
[20]黄涛,孔庆志,卢宏达,等.槐耳清膏对耐顺铂人肺腺癌细胞系A549DDP逆转的实验研究[J].中国药师,2002,5(9):517-521.
[21]崔洋,刘伟东,史进,等.槐耳清膏对骨肉瘤MG63细胞凋亡、迁移和侵袭的影响[J].中华临床医师杂志(电子版),2013,7(12):5415-5419.
[2]陈万清,郑荣寿,张思维,等. 2012年中国恶性肿瘤发病与死亡分析[J].中国肿瘤,2016,25(1):1-8.
[3]吴一龙,秦叔逵,马军.中国临床肿瘤学进展2015[M].北京:人民卫生出版社,2015:105-107.
[4]ZHANG N,KONG X L,YAN S,et al. Huaier aqueous extract inhibits proliferation of breast cancer cells by inducing apoptosis[J]. Cancer Sci,2010,101(11):2375-2383.
[5]海艳杰,郑宇,庄亚严,等.槐耳颗粒联合化疗对晚期大肠癌的初步临床研究[J].药物流行病学杂志,2012,21(2):53-55.
[6]鲁明骞,冯雪松,孔庆志,等.槐耳颗粒对乳腺癌术后化疗患者免疫功能及生活质量的影响[J].东南大学学报(医学版),2017,36(4):567-571.
[7]LV H L,JIANG Y F,LIAO M,et al. In vitro and in vivo treatments of echinococcus granulosus with huaier aqueous extract and albendazole liposome[J]. Parasitol Res,2013,112(1):193-198.
[8]鲁明骞,孔庆志.槐耳制剂治疗乳腺癌的作用及机理研究[J].湖北中医药大学学报,2015,17(5):101-103.
[9]TAYLOR S G,GELMAN R S,FALKSON G,et al. Combination chemotherapy compared to tamosifen as initial therapy for stageⅣbreast cancr in elderly women[J]. Ann Intern Med,1986,104(4):455-461.
[10]WAKELING A E,DEKES M,BOWLER J. A potent specific pure antiestrogen with clinical potential[J]. Cancer Res,1991,51(15):3867-3873.
[11]ARTEAGA C L,ENGELMAN J. ERBB receptors:from oncogene discovery to basic science to mechanism-based cancer therapeutics[J]. Cancer Cell,2014,25(3):282-303.
[12] IGNATIADIS M,SOTIRIOU C. Luminal breast cancer:from biology to treatment[J]. Nat Rev Clin Oncol,2013,10(9):494-506.
[13]OSBORNE C K,SCHIFF R. Mechanisms of endocrine resistance in breast cancer[J]. Annu Rev Med,2011,62:233-247.
[14]DHILON S. Everolimus in combination with exemestane:a review of its use in the treatmentof patients with postmenopausal hormone receptor-positive,HER2-negative advanced breast cancer[J]. Drugs,2013,73(5):475-485.
[15]HOUGHTON P J. Everolimus[J]. Clin Cancer Res,2010,16(5):1368-1372.
[16]KUO P L,CHIANG,L C,LIN C C. Resveratrol-induced apoptosis is mediated by p53-dependent pathway in Hep G2 cells[J]. Life Sci,2002,72(1):23-24.
[17]鲁明骞,卢宏达,孔庆志.槐耳治疗恶性肿瘤的研究进展[J].中国中医基础医学杂志,2015,21(12):1607-1609.
[18]张芷旋,范羽,周清华,等.槐耳清膏对人高转移大细胞肺癌细胞L9981血管生成相关基因表达的影响[J].中国肺癌杂志,2006,9(2):137-142.
[19]JIN F P,ZHANG M. Progress of experimental researches on Chinese herbal compounds for inducing tumor cell apoptosis[J]. Chin J Integr Med,2010,16(6):565-571.
[20]黄涛,孔庆志,卢宏达,等.槐耳清膏对耐顺铂人肺腺癌细胞系A549DDP逆转的实验研究[J].中国药师,2002,5(9):517-521.
[21]崔洋,刘伟东,史进,等.槐耳清膏对骨肉瘤MG63细胞凋亡、迁移和侵袭的影响[J].中华临床医师杂志(电子版),2013,7(12):5415-5419.