疏肝健脾方水提工艺的正交试验法结合UPLC优选
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摘要
目的优选疏肝健脾方的最佳水提工艺。方法采用正交试验法结合超高效液相色谱(UPLC)技术,以黄芪甲苷含量、干浸膏得率为评价指标,通过L_9(3~4)正交试验法,考察加水量、提取时间、提取次数对疏肝健脾方水提工艺的影响。结果疏肝健脾方最佳水提工艺为加8倍量水,提取3次,每次1.5h。结论优选出的最佳提取工艺稳定、可行,为疏肝健脾方的工业化生产提供了可靠的实验依据。
Objective To optimize the water- extraction process of Shu Gan Jian Pi Formula. Methods The L_9( 3~4) orthogonal design combining UPLC were applied with the content of total astragaloside and dry extract yield as evaluation index,water- extraction technology of Shu Gan Jian Pi Formula was optimized by the amount of water,extraction times and extraction time. Results The optimum technology of water extraction was that Shu Gan Jian Pi Formula was extracted with 8 times the amount of water for 3 times,1. 5 hours each time. Conclusion The optimum water- extraction technology was stable,feasible and provided experimental basis for the industrial production of Shu Gan Jian Pi Formula.
Objective To optimize the water- extraction process of Shu Gan Jian Pi Formula. Methods The L_9( 3~4) orthogonal design combining UPLC were applied with the content of total astragaloside and dry extract yield as evaluation index,water- extraction technology of Shu Gan Jian Pi Formula was optimized by the amount of water,extraction times and extraction time. Results The optimum technology of water extraction was that Shu Gan Jian Pi Formula was extracted with 8 times the amount of water for 3 times,1. 5 hours each time. Conclusion The optimum water- extraction technology was stable,feasible and provided experimental basis for the industrial production of Shu Gan Jian Pi Formula.
引文
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[2]曹承楼,杨素霞,张国梁,等.肝乐冲剂治疗慢性病毒性肝炎肝郁脾虚证疗效观察[J].河南中医,2007,27(10):28.
[3]姜辉,吴芙蓉,高家荣,等.疏肝健脾方对CCl4致大鼠肝纤维化的治疗作用动态观察[J].中药材,2014,37(10):1815.
[4]马雪松,谭蔚,李莉.正交试验法优选黄芪甲苷的提取工艺[J].河北科技大学学报,2006,27(1):57.
[5]陈永祥,孙耀志,高松,等.正交试验优选黄芪桂枝五物汤的水提取工艺[J].中国实验方剂学杂志,2012,18(2):56.
[6]庄华玲,黄亚非,张永明.优选黄芪甲苷的水煎煮提取工艺[J].中药材,2006,29(12):1363.
[7]薛东升.益气降浊胶囊提取工艺研究[J].中成药,2010,32(10):1812.
[8]杨连梅,刘养清,赵慧辉,等.黄芪药材中黄芪甲苷含量的荧光分光光度法测定[J].时珍国医国药,2010,21(9):2272.
[9]张丽,佟巍,龚海英,等.双波长薄层色谱扫描法测定复方黄芪胶囊中黄芪甲苷的含量[J].武警医学院学报,2008,17(6):492.
[10]孙聪,韩业超,吴强.高效液相色谱法测定保元清血颗粒中黄芪甲苷含量方法的建立及评价[J].吉林大学学报,2012,38(4):806.