养殖克氏原螯虾不同季节稳定同位素特征
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摘要
为了更好地对克氏原螯虾进行育种和养殖,对江苏省淮安区南闸镇水产养殖基地中夏季和秋季的克氏原螯虾作生物学分析和稳定同位素分析。其中,有效试验龙虾共90尾,体长范围在6.48～12.88 cm,其中夏季和秋季克氏原螯虾体长、体质量、头胸甲之间差异均不显著。克氏原螯虾δ~(13)C值范围为-28.97～-19.03(n=90);δ~(15)N值范围为4.86～14.30(n=90)。按不同体长将克氏原螯虾进行分组,发现体型较小的组与体型较大的组之间稳定同位素值均存在显著差异(P<0.05),而不同性别之间的克氏原螯虾稳定同位素值不存在差异。不同季节之间雌性克氏原螯虾δ~(13)C值之间没有差异,而δ~(15)N值存在显著差异(P<0.05);不同季节雄性克氏原螯虾δ~(13)C值和δ~(15)N值均存在显著差异(P<0.05),并且δ~(15)N值差异极显著(P<0.01)。通过对克氏原螯虾的生物学特征和稳定同位素特征进行分析,可以更好地了解养殖环境中克氏原螯虾的生长情况以及摄食状况,从而能够根据克氏原螯虾的生长习性和生活习惯进行养殖改进,为克氏原螯虾的育种和养殖提供数据支持和指导作用。
引文
[1]李林春,段鸿斌. 克氏螯虾(龙虾)生物学特性研究[J]. 安徽农业科学,2005,33(6):1058-1059.
[2]徐增洪,周鑫,水燕. 克氏原螯虾的食物选择性及其摄食节律[J]. 大连海洋大学学报,2012,27(2):166-170.
[3]郭晓鸣,朱松泉. 克氏原螯虾幼体发育的初步研究[J]. 动物学报,1997,43(4):372-381.
[4]温小波,库夭梅,罗静波. 克氏原螯虾耗氧率及窒息点的研究[J]. 大连水产学院学报,2003,18(3):170-174.
[5]李云凯,贡艺,陈新军. 稳定同位素技术在头足类摄食生态学研究中的应用[J]. 应用生态学报,2014,25(5):1541-1546.
[6]Hertz E,Trudel M,Tucker S,et al. Overwinter shifts in the feeding ecology of juvenile Chinook salmon[J]. ICES Journal of Marine Science,2017,74(1):226-233.
[7]Sulak K J,Berg J J,Randall M. Feeding habitats of the Gulf sturgeon,Acipenser oxyrinchus desotoi,in the Suwannee and Yellow Rivers,Florida,as identified by multiple stable isotope analyses[J]. Environmental Biology of Fishes,2012,95(2):237-258.
[8]朱国平,朱小艳. 南极磷虾种群生物学研究进展Ⅲ——摄食[J]. 水生生物学报,2014,38(6):1152-1160.
[9]贡艺,陈新军,高春霞,等. 脂类抽提对北太平洋柔鱼肌肉碳、氮稳定同位素测定结果的影响[J]. 应用生态学报,2014,25(11):3349-3356.
[10]Larson E R,Twardochleb L A,Olden J D. Comparison of trophic function between the globally invasive crayfishes Pacifastacus leniusculus and Procambarus clarkii[J]. Limnology,2017,18(3):275-286.
[11]Glon M G,Larson E R,Pangle K L. Comparison of 13C and 15N discrimination factors and turnover rates between congeneric crayfish Orconectes rusticus and O. virilis(Decapoda,Cambaridae)[J]. Hydrobiologia,2016,768(1):51-61.
[12]Rosewarne P J,Mortimer R J,Newton R J,et al. Feeding behaviour,predatory functional responses and trophic interactions of the invasive Chinese mitten crab (Eriocheir sinensis) and signal crayfish (Pacifastacus leniusculus)[J]. Freshwater Biology,2016,61(4):426-443.
[13]Mao Z G,Gu X H,Zeng Q F. Food sources and trophic relationships of three decapod crustaceans:insights from gut contents and stable isotope analyses[J]. Aquaculture Research,2016,47(9):2888-2898.
[14]Guerao G,Rotllant G,Gisbert E,et al. Consistent habitat segregation between sexes in the spider crabs Maja brachydactyla and Maja squinado (Brachyura),as revealed by stable isotopes[J]. Scientia Marina,2016,80(1):103-110.
[15]Divine L M,Bluhm B A,Mueter F J,et al. Diet analysis of Alaska Arctic snow crabs(Chionoecetes opilio)using stomach contents and δ13C and δ15N stable isotopes[J]. Deep-Sea Research Part Ⅱ-Topical Studies in Oceanography,2017,135:124-136.
[16]Careddu G,Calizza E,Costantini M L. Isotopic determination of the trophic ecology of a ubiquitous key species-the crab Liocarcinus depurator (Brachyura:Portunidae)[J]. Estuarine Coastal and Shelf Science,2017,191:106-114.
[17]管卫兵,苏孙国,何文辉. 青草沙水库立体复合生态操纵池塘水生动植物的同位素特征[J]. 海洋湖沼通报,2015(1):41-49.
[18]Budge S M,Iverson S J,Koopman H N. Studying trophic ecology in marine ecosystems using fatty acids:a primer on analysis and interpretation[J]. Marine Mammal Science,2006,22(4):759-801.
[19]Hatase H,Takai N,Yoshimasa M,et al. Size-related differences in feeding habitat use of adult female loggerhead turtles[J]. Marine Ecology Progress,2002,233(1):273-281.
[20]Geffen A J. Otolith oxygen and carbon stable isotopes in wild and laboratory-reared plaice (Pleuronectes platessa)[J]. Environmental Biology of Fishes,2012,95(4):419-430.
[21]Suh Y J,Shin K H. Size-related and seasonal diet of the manila clam (Ruditapes philippinarum),as determined using dual stable isotopes[J]. Estuarine Coastal and Shelf Science,2013,135:94-105.
[22]Gherardi F,Barbaresi S. Feeding preferences of the invasive crayfish,Procambarus clarkii[J]. Knowledge and Management of Aquatic Ecosystems,2007,385(387):7-20.
[23]谢文星,董方勇,谢山. 克氏原螯虾的食性、繁殖和栖息习性研究[J]. 水利渔业,2008,28(4):63-65.
[24]彭刚,陈大鹏,黄鸿兵,等. 日本沼虾对克氏原螯虾幼虾胁迫试验[J]. 江苏农业科学,2017,45(21):190-192.
[25]Tomida Y,Suzuki T,Yamada T,et al. Differences in oxygen and carbon stable isotope ratios between hatchery and wild pink salmon fry[J]. Fisheries Science,2014,80(2):273-280.
[26]Sun Z L,Gao Q F,Dong S L,et al. Seasonal changes in food uptake by the sea cucumber Apostichopus japonicus in a farm pond:evidence from C and N stable isotopes[J]. Journal of Ocean University of China,2013,12(1):160-168.
[27]Stenroth P,Holmqvist N,Nystr?m P,et al. Stable isotopes as an indicator of diet in omnivorous crayfish(Pacifastacus leniusculus):the influence of tissue,sample treatment,and season[J]. Canadian Journal of Fisheries and Aquatic Sciences,2006,63(4):821-831.伍冰倩,刘妮妮,杨鑫豪,等. 新型免疫分子TRIM23参与斑马鱼抗嗜水气单胞菌免疫应答[J]. 江苏农业科学,2019,47(4):153-156.
[2]徐增洪,周鑫,水燕. 克氏原螯虾的食物选择性及其摄食节律[J]. 大连海洋大学学报,2012,27(2):166-170.
[3]郭晓鸣,朱松泉. 克氏原螯虾幼体发育的初步研究[J]. 动物学报,1997,43(4):372-381.
[4]温小波,库夭梅,罗静波. 克氏原螯虾耗氧率及窒息点的研究[J]. 大连水产学院学报,2003,18(3):170-174.
[5]李云凯,贡艺,陈新军. 稳定同位素技术在头足类摄食生态学研究中的应用[J]. 应用生态学报,2014,25(5):1541-1546.
[6]Hertz E,Trudel M,Tucker S,et al. Overwinter shifts in the feeding ecology of juvenile Chinook salmon[J]. ICES Journal of Marine Science,2017,74(1):226-233.
[7]Sulak K J,Berg J J,Randall M. Feeding habitats of the Gulf sturgeon,Acipenser oxyrinchus desotoi,in the Suwannee and Yellow Rivers,Florida,as identified by multiple stable isotope analyses[J]. Environmental Biology of Fishes,2012,95(2):237-258.
[8]朱国平,朱小艳. 南极磷虾种群生物学研究进展Ⅲ——摄食[J]. 水生生物学报,2014,38(6):1152-1160.
[9]贡艺,陈新军,高春霞,等. 脂类抽提对北太平洋柔鱼肌肉碳、氮稳定同位素测定结果的影响[J]. 应用生态学报,2014,25(11):3349-3356.
[10]Larson E R,Twardochleb L A,Olden J D. Comparison of trophic function between the globally invasive crayfishes Pacifastacus leniusculus and Procambarus clarkii[J]. Limnology,2017,18(3):275-286.
[11]Glon M G,Larson E R,Pangle K L. Comparison of 13C and 15N discrimination factors and turnover rates between congeneric crayfish Orconectes rusticus and O. virilis(Decapoda,Cambaridae)[J]. Hydrobiologia,2016,768(1):51-61.
[12]Rosewarne P J,Mortimer R J,Newton R J,et al. Feeding behaviour,predatory functional responses and trophic interactions of the invasive Chinese mitten crab (Eriocheir sinensis) and signal crayfish (Pacifastacus leniusculus)[J]. Freshwater Biology,2016,61(4):426-443.
[13]Mao Z G,Gu X H,Zeng Q F. Food sources and trophic relationships of three decapod crustaceans:insights from gut contents and stable isotope analyses[J]. Aquaculture Research,2016,47(9):2888-2898.
[14]Guerao G,Rotllant G,Gisbert E,et al. Consistent habitat segregation between sexes in the spider crabs Maja brachydactyla and Maja squinado (Brachyura),as revealed by stable isotopes[J]. Scientia Marina,2016,80(1):103-110.
[15]Divine L M,Bluhm B A,Mueter F J,et al. Diet analysis of Alaska Arctic snow crabs(Chionoecetes opilio)using stomach contents and δ13C and δ15N stable isotopes[J]. Deep-Sea Research Part Ⅱ-Topical Studies in Oceanography,2017,135:124-136.
[16]Careddu G,Calizza E,Costantini M L. Isotopic determination of the trophic ecology of a ubiquitous key species-the crab Liocarcinus depurator (Brachyura:Portunidae)[J]. Estuarine Coastal and Shelf Science,2017,191:106-114.
[17]管卫兵,苏孙国,何文辉. 青草沙水库立体复合生态操纵池塘水生动植物的同位素特征[J]. 海洋湖沼通报,2015(1):41-49.
[18]Budge S M,Iverson S J,Koopman H N. Studying trophic ecology in marine ecosystems using fatty acids:a primer on analysis and interpretation[J]. Marine Mammal Science,2006,22(4):759-801.
[19]Hatase H,Takai N,Yoshimasa M,et al. Size-related differences in feeding habitat use of adult female loggerhead turtles[J]. Marine Ecology Progress,2002,233(1):273-281.
[20]Geffen A J. Otolith oxygen and carbon stable isotopes in wild and laboratory-reared plaice (Pleuronectes platessa)[J]. Environmental Biology of Fishes,2012,95(4):419-430.
[21]Suh Y J,Shin K H. Size-related and seasonal diet of the manila clam (Ruditapes philippinarum),as determined using dual stable isotopes[J]. Estuarine Coastal and Shelf Science,2013,135:94-105.
[22]Gherardi F,Barbaresi S. Feeding preferences of the invasive crayfish,Procambarus clarkii[J]. Knowledge and Management of Aquatic Ecosystems,2007,385(387):7-20.
[23]谢文星,董方勇,谢山. 克氏原螯虾的食性、繁殖和栖息习性研究[J]. 水利渔业,2008,28(4):63-65.
[24]彭刚,陈大鹏,黄鸿兵,等. 日本沼虾对克氏原螯虾幼虾胁迫试验[J]. 江苏农业科学,2017,45(21):190-192.
[25]Tomida Y,Suzuki T,Yamada T,et al. Differences in oxygen and carbon stable isotope ratios between hatchery and wild pink salmon fry[J]. Fisheries Science,2014,80(2):273-280.
[26]Sun Z L,Gao Q F,Dong S L,et al. Seasonal changes in food uptake by the sea cucumber Apostichopus japonicus in a farm pond:evidence from C and N stable isotopes[J]. Journal of Ocean University of China,2013,12(1):160-168.
[27]Stenroth P,Holmqvist N,Nystr?m P,et al. Stable isotopes as an indicator of diet in omnivorous crayfish(Pacifastacus leniusculus):the influence of tissue,sample treatment,and season[J]. Canadian Journal of Fisheries and Aquatic Sciences,2006,63(4):821-831.伍冰倩,刘妮妮,杨鑫豪,等. 新型免疫分子TRIM23参与斑马鱼抗嗜水气单胞菌免疫应答[J]. 江苏农业科学,2019,47(4):153-156.