摘要
将3株冲天湖底泥优势菌(Exiguobacterium.sp013、Bacillus.spD06与Bacillus.spD24)按不同配比与铜绿微囊藻休眠体用底泥包埋,在梯度温度下(10、15、20和25℃)进行复苏模拟实验.结果表明,在15、20和25℃条件下,菌组和无菌组铜绿微囊藻休眠体均能从底泥复苏进入上覆水体,但菌组复苏率显著高于无菌组(p<0.05).在10℃条件下,菌组铜绿微囊藻休眠体能复苏,而无菌组不能.在20和25℃时,菌组对铜绿微囊藻休眠体光合系统的光合效率F_v/F_m无显著提升作用(p> 0.05),而在10和15℃时,菌组能显著提升铜绿微囊藻休眠体的光合效率F_v/F_m(p<0.05),且菌组Mix-4的提升能力显著强于其他菌组和无菌组.基因测序和KEGG富集分析发现,15℃时菌组Mix-4差异表达的基因显著富集于Cell cycle通路中,包括氧化磷酸化(ATP生成反应)通路和内质网蛋白合成通路,同时与光合作用相关的蛋白基因psaB、psbA及rbcL的表达量均发生显著上调,这可能是Mix-4菌群在较低温度下(15℃)提升铜绿微囊藻休眠体光合效率F_v/F_m并促进其复苏的分子机理.
To explore the effects of the three dominant benthic freshwater bacteria( Exiguobacterium.sp013,Bacillus.spD06 and Bacillus.spD24) on the recruitment of dormant Microcystis aeruginosa from the sediment,and to find potential factors and mechanism,simulation on the recruitment of M. aeruginosa cells from sediment were performed in the presence or absence of the three bacterial strains. Three strains of the dominant bacteria isolated from Lake Chongtian and dormant M. aeruginosa were embedded in the sediment according to different bacterial ratio,and the recruitment experiments were conducted under different temperatures(10,15,20 and 25 ℃). Results show that the dormant M. aeruginosa cells in both of the bacterial and aseptic groups recruited from the sediment into the overlying water under the temperature of 15,20 and 25 ℃,and the recruitment rate of bacterial groups were significantly higher than that of aseptic groups( p<0.05). At 10 ℃,the recruitment of dormant M. aeruginosa cells takes place in bacterial groups,but not in aseptic groups. At 10 ℃ and 15 ℃,the photosynthetic efficiency( F_v/F_m) of the dormant M. aeruginosa were significantly enhanced by mixed bacterial group( Mix-4),which was significantly stronger than other bacterial and aseptic group. When the temperature was 20 ℃ and 25 ℃,there were no significant effect on the photosynthetic efficiency( F_v/F_m) of dormant M. aeruginosa cells(p>0.05) induced by mixed bacterial group. Then,the differentially expressed genes were found that were significantly enriched in the cell cycle pathway,including the oxidative phosphorylation( ATP generation reaction) pathway and endoplasmic reticulum protein synthesis pathway at 15 ℃ for Mix-4 groups by gene sequencing and KEGG enrichment analysis. At the same time,the expression levels of psa B,psb A and rbc L genes related to photosynthesis significantly increased( UP). These might be the molecular mechanism,which mixed bacteria Mix-4 enhanced photosynthetic efficiency( F_v/F_m) of dormant M.aeruginosa at lower temperature(15 ℃)and promoted its recruitment.
引文
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