摘要
用硬模板法制得具有三维连续贯通孔道结构的整体型大孔/介孔SiO_2,通过多巴胺(DA)在大孔/介孔SiO_2孔道表面的原位氧化聚合,制得聚多巴胺(PDA)功能化修饰的整体型大孔/介孔复合材料(PDA/SiO_2)。应用SEM、BET、FTIR和TG等技术对修饰前后的材料进行表征。以PDA/SiO_2为载体固定诺维信工业级漆酶,系统研究了pH、固定化时间、漆酶初始浓度及温度对漆酶固定化的影响;以偶氮荧光桃红作为模拟污染物,研究了固定化漆酶对染料的催化降解性能。结果显示,在漆酶浓度为80mg/mL、pH为4.0、固定化时间为6h及固定化温度为25℃时,固定化漆酶酶活达到最高(348.9U/g)。在偶氮荧光桃红浓度为10mg/L、pH为7.0、温度为30℃、降解时间为8h时,固定化漆酶对偶氮荧光桃红脱色率≥99.9%,且固定化漆酶易从反应体系中分离,重复使用性能良好。
A monolithic macroporous/mesoporous SiO_2 with three-dimensional(3D) continuous passthrough pore structure was prepared by the hard template method. It was the surface functionalized with polydopamine(PDA) by the in situ oxidation polymerization of dopamine in its micro-channels to obtainthe macroporous/mesoporous composite PDA/SiO_2. Samples were characterized by SEM,BET,FTIR andTG. PDA/SiO_2 was used to immobilize commercial Novozymes laccase; and the effects of pH,immobilizingtime,initial concentration of laccase and temperature on laccase immobilization were investigated. Usingazophloxine as a simulated pollutant, the catalytic performance of the immobilized laccase for thedegradation of dye was studied. The results showed that the activity of immobilized laccase reached themaximum(348.9U/g) under conditions of initial laccase concentration 80mg/mL,pH of 4.0,immobilizingtime of 6h and temperature of 25℃. Up to 99.9% of azophloxine was decolored under azophloxine concentration 10mg/L,pH of 7.0,temperature of 30℃,and degradation time of 8h.The immobilized laccase exhibited good reusability and it can be easily recovered from the reaction system.
引文
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