茉莉酸甲酯对艾纳香活性成分、抗氧化酶活力以及内源激素含量的影响
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摘要
目的研究艾纳香植株不同叶位叶片中4种内源激素含量和3种抗氧化酶活性以及L-龙脑的含量与诱导剂的浓度、采样时间之间的规律。方法以0.01、0.10、1.00、10.00mmol/L的茉莉酸甲酯(MeJA)作为外源诱导剂,艾纳香不同叶位的叶片(嫩叶、成熟叶、老叶)为实验对象,以生长素(IAA)、脱落酸(ABA)、赤霉素(GA3)、玉米素(ZT)4种内源激素含量和过氧化物酶(POD)、过氧化氢酶(CAT)、超氧化物歧化酶(SOD)3种抗氧化酶的活力以及活性成分L-龙脑的含量作为检测指标。结果 1.00 mmol/L的MeJA对L-龙脑的积累效果较好;不同浓度的MeJA诱导下,抗氧化酶的变化比较复杂。对于POD来说,除了10.00 mmol/L MeJA浓度处理下的艾纳香3个叶位的叶片中其含量低于对照外,其他浓度处理下,POD在72 h均显著高于对照(P<0.05)。对于CAT来说,10.00 mmol/L MeJA诱导下,艾纳香3个叶位叶片其含量在24 h时达到最高,之后随着时间的推移,CAT活力极速下降。在其他浓度处理下,嫩叶和老叶中的CAT酶活力在72 h,显著低于对照,而在成熟叶中除了0.10 mmol/L MeJA处理,均在72 h时高于对照。对于SOD来说,除了1.00 mmol/L MeJA处理的3个叶位的叶片在48 h之后,SOD含量均高于对照,且与对照组有显著差异(P<0.05)外,其余浓度处理下的SOD均低于对照。低浓度的MeJA(≤0.10 mmol/L)可以促进艾纳香叶片中的IAA、GA3和ZT的积累,而高浓度的MeJA(≥10.00 mmol/L)促进ABA的积累。结论艾纳香植株在外源MeJA(1.00 mmol/L)诱导下可以促进活性成分积累,为其栽培生产提供理论依据。
Objective To study the regulation among the content of L-borneol and four endogenous hormones and the activity of three anti-oxidant enzymes in Blumea balsamifera leaves located at different leaf positions and the concentration of inducer and the sampling time. Methods Methyle Jasmonate(MeJA) of 0.01 mmol/L, 0.10 mmol/L, 1.00 mmol/L, and 10.00 mmol/L was chosen for exogenous inducer in this experiment. The leaves of B. balsamifera located at different leaf positions(tender leaves, mature leaves, aged leaves) were experimental materials. The active content of L-borneol, the content of four endogenous hormones of auxin(IAA), abscisic acid(ABA), gibberellic acid(GA3), and zeatin(ZT), and the activity of three antioxidant enzymes of peroxidase(POD), catalase(CAT), and superoxide dismutase(SOD) were detection indexes. Results The results showed that the effect of 1.00 mmol/L MeJA on the accumulation of L-borneol was good. The changes of anti-oxidant enzymes induced by different concentrations of MeJA were complex. For the content of POD, except that the B. balsamifera leaves treated with 10.00 mmol/L MeJA were lower than that in the control, POD were significantly higher than those of the control(P < 0.05) at 72 h in other conditions. Under the induction of 10.00 mmol/L MeJA, the CAT content of the B. balsamifera leaves at three leaf positions was highest at 24 h, and the activity of CAT was decreased rapidly over time. Under the MeJA treatments of other concentrations, the activities of CAT in young leaves and old leaves were significantly lower than those in the control at 72 h, but higher than those in the control at 72 h except for the treatment of 0.1 mmol/L MeJA in mature leaves. The content of SOD in the three leaf positions was lower than the control except that the B. balsamifera leaves treated with 1 mmol/L MeJA was significantly higher than that of the control after 48 h. The rest of the concentration of superoxide dismutase were lower than the control. Low concentration of MeJA(≤ 0.10 mmol/L) could promote the accumulation of IAA, GA3, and ZT in leaves of B. balsamifera, whereas the high concentration of MeJA(≥ 10.00 mmol/L) could promote the accumulation of ABA. Conclusion Under the induction of exogenous MeJA(1.00 mmol/L), B. balsamifera can promote the accumulation of active ingredients, providing a theoretical basis for its cultivation and production.
Objective To study the regulation among the content of L-borneol and four endogenous hormones and the activity of three anti-oxidant enzymes in Blumea balsamifera leaves located at different leaf positions and the concentration of inducer and the sampling time. Methods Methyle Jasmonate(MeJA) of 0.01 mmol/L, 0.10 mmol/L, 1.00 mmol/L, and 10.00 mmol/L was chosen for exogenous inducer in this experiment. The leaves of B. balsamifera located at different leaf positions(tender leaves, mature leaves, aged leaves) were experimental materials. The active content of L-borneol, the content of four endogenous hormones of auxin(IAA), abscisic acid(ABA), gibberellic acid(GA3), and zeatin(ZT), and the activity of three antioxidant enzymes of peroxidase(POD), catalase(CAT), and superoxide dismutase(SOD) were detection indexes. Results The results showed that the effect of 1.00 mmol/L MeJA on the accumulation of L-borneol was good. The changes of anti-oxidant enzymes induced by different concentrations of MeJA were complex. For the content of POD, except that the B. balsamifera leaves treated with 10.00 mmol/L MeJA were lower than that in the control, POD were significantly higher than those of the control(P < 0.05) at 72 h in other conditions. Under the induction of 10.00 mmol/L MeJA, the CAT content of the B. balsamifera leaves at three leaf positions was highest at 24 h, and the activity of CAT was decreased rapidly over time. Under the MeJA treatments of other concentrations, the activities of CAT in young leaves and old leaves were significantly lower than those in the control at 72 h, but higher than those in the control at 72 h except for the treatment of 0.1 mmol/L MeJA in mature leaves. The content of SOD in the three leaf positions was lower than the control except that the B. balsamifera leaves treated with 1 mmol/L MeJA was significantly higher than that of the control after 48 h. The rest of the concentration of superoxide dismutase were lower than the control. Low concentration of MeJA(≤ 0.10 mmol/L) could promote the accumulation of IAA, GA3, and ZT in leaves of B. balsamifera, whereas the high concentration of MeJA(≥ 10.00 mmol/L) could promote the accumulation of ABA. Conclusion Under the induction of exogenous MeJA(1.00 mmol/L), B. balsamifera can promote the accumulation of active ingredients, providing a theoretical basis for its cultivation and production.
引文
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[12]杜娟,郭华春.马铃薯品种中甸红实生种子发芽率和内源激素水平的相关性分析[J].云南农业大学学报2015,30(2):198-202.
[13]Zeng Q Q,Chen H B,Lu C H,et al.An optimized HPLCprocedure for analyzing endogenous hormones in different organs of litchi[J].J Fruit Sci,2006,23(1):145-148.
[14]Loreto F.Distribution of isoprenoid emitters in the Quercus genus around the world:Chemo-taxonomical implications and evolutionary considerations based on the ecological function of the trait[J].Perspect Plant Ecol,2002,5(3):185-192.
[15]付晓莹,郭慧敏,丛微,等.外源H2O2对黄芩次生代谢调控及道地质量形成机制研究[J].中国中药杂志,2017,doi:10.19540/j.cnki.cjcmm.20171030.009.
[2]施江.外源茉莉酸甲酯诱导对茶树鲜叶次生代谢产物的影响[D].北京:中国农业科学院,2014.
[3]李文渊,高伟,赵静,等.基于茉莉酸甲酯诱导的丹参毛状根酚酸类成分次生代谢机制研究[J].中国中药杂志,2012,37(1):13-16.
[4]于萌萌,申琳,生吉萍.茉莉酸甲酯诱导采后番茄果实抗病的作用[J].食品科学,2012(9):11-15.
[5]吴莹莹,黄凤宽,韦素美,等.茉莉酸甲酯诱导水稻后对褐飞虱的抗性研究[J].广西农业科学,2008(4):474-477.
[6]路文静.植物生理学[M].北京:中国林业出版社,2011.
[7]段娜,贾玉奎,徐军,等.植物内源激素研究进展[J].中国农学通报,2015,31(2):159-165.
[8]王学奎.植物生理生化实验原理和技术[M].第2版.北京:高等教育出版社,2006.
[9]徐应文.鱼腥草单萜次生代谢研究[D].雅安:四川农业大学,2012.
[10]侯凯.川白芷资源评价与植物激素对其生长发育和产量品质的影响[D].雅安:四川农业大学,2013.
[11]刘世红,李平生,陈国华,等.橡胶树老幼态芽内源激素差异分析[J].中国热带农业,2015,66(5):51-54.
[12]杜娟,郭华春.马铃薯品种中甸红实生种子发芽率和内源激素水平的相关性分析[J].云南农业大学学报2015,30(2):198-202.
[13]Zeng Q Q,Chen H B,Lu C H,et al.An optimized HPLCprocedure for analyzing endogenous hormones in different organs of litchi[J].J Fruit Sci,2006,23(1):145-148.
[14]Loreto F.Distribution of isoprenoid emitters in the Quercus genus around the world:Chemo-taxonomical implications and evolutionary considerations based on the ecological function of the trait[J].Perspect Plant Ecol,2002,5(3):185-192.
[15]付晓莹,郭慧敏,丛微,等.外源H2O2对黄芩次生代谢调控及道地质量形成机制研究[J].中国中药杂志,2017,doi:10.19540/j.cnki.cjcmm.20171030.009.