宫颈癌患者血清微小RNA-196a表达变化及意义
摘要
目的观察宫颈癌患者血清微小RNA-196a(miR-196a)的表达变化,并探讨其意义。方法选取2014年1～12月收诊的80例宫颈癌患者,同时选取同期进行健康体检的80例健康志愿者,采用TaqMan实时荧光定量PCR检测入选受试者血清miR-196a表达,比较宫颈癌患者与健康志愿者血清miR-196a表达,并分析宫颈癌患者血清miR-196a表达与临床病理特征的关系。对宫颈癌患者的预后情况进行随访调查,分析不同血清miR-196a表达宫颈癌患者的生存情况。以受试者工作特征(ROC)曲线评估血清miR-196a用于宫颈癌诊断的灵敏度、特异度。结果宫颈癌患者血清miR-196a表达高于健康志愿者(t=12.082,P=0.000)。宫颈癌患者血清miR-196a表达与肿瘤直径、淋巴结转移和FIGO分期有关(t分别为2.035、-2.481、2.046,P分别为0.045、0.015、0.044)。42例宫颈癌患者血清miR-196a相对表达量高于平均值(miR-196a高表达组),38例宫颈癌患者血清miR-196a相对表达量低于平均值(miR-196a低表达组)。与血清miR-196a低表达组相比,血清miR-196a高表达组的3年生存率降低(χ~2=5.082,P=0.024)。ROC曲线分析结果显示,血清miR-196a诊断宫颈癌的ROC曲线下面积为0.853,对宫颈癌诊断的灵敏度和特异度分别为0.832和0.765。结论宫颈癌患者血清miR-196a表达升高,且与肿瘤的恶性程度、患者预后相关;检测血清miR-196a表达有助于宫颈癌诊断、病情预测及预后判断。
引文
[1] 张媛,贺桂芳,卞美璐,等.生物标记物对于宫颈癌的诊治和研究进展[J].中日友好医院学报,2017,31(5):307-309.
[2] 孙娟娟,宋晓婕,罗自娟,等.高危型HPV筛查与TCT联合检查在宫颈癌筛查中的临床应用[J].现代生物医学进展,2016,16(12):2336-2338,2263.
[3] Hayes J,Peruzzi PP,Lawler S.MicroRNAs in cancer:biomarkers,functions and therapy[J].Trends Mol Med,2014,20(8):460-469.
[4] Chen TH,Lee C,Chiu CT,et al.Circulating microRNA-196a is an early gastric cancer biomarker[J].Oncotarget,2017,9(12):10317-10323.
[5] Yang L,Peng F,Qin J,et al.Downregulation of microRNA-196a inhibits human liver cancer cell proliferation and invasion by targeting FOXO1[J].Oncol Rep,2017,38(4):2148-2154.
[6] Chen H,Fan Y,Xu W,et al.Exploration of miR-1202 and miR-196a in human endometrial cancer based on high throughout gene screening analysis[J].Oncol Rep,2017,37(6):3493-3501.
[7] Xu YF,Hannafon BN,Zhao YD,et al.Plasma exosome miR-196a and miR-1246 are potential indicators of localized pancreatic cancer[J].Oncotarget,2017,8(44):77028-77040.
[8] Liu P,Xin F,Ma CF.Clinical significance of serum miR-196a in cervical intraepithelial neoplasia and cervical cancer[J].Genet Mol Res,2015,14(4):17995-18002.
[9] 李慧聪.miR-196a高表达在肾脏损伤中作用的分子生物学机制[J].中国老年学杂志,2018,38(10):2472-2475.
[10] Yang B,Li SZ,Ma L,et al.Expression and mechanism of action of miR-196a in epithelial ovarian cancer[J].Asian Pac J Trop Med,2016,9(11):1105-1110.
[11] Jiang CF,Shi ZM,Li DM,et al.Estrogen-induced miR-196a elevation promotes tumor growth and metastasis via targeting SPRED1 in breast cancer[J].Mol Cancer,2018,17(1):83.
[12] Lu YC,Chang JT,Huang YC,et al.Combined determination of circulating miR-196a and miR-196b levels produces high sensitivity and specificity for early detection of oral cancer[J].Clin Biochem,2015,48(3):115-121.
[13] Zhang C,Yao C,Li H,et al.Combined elevation of microRNA-196a and microRNA-196b in sera predicts unfavorable prognosis in patients with osteosarcomas[J].Int J Mol Sci,2014,15(4):6544-6555.
[14] Gocze K,Gombos K,Juhasz K,et al.Unique microRNA expression profiles in cervical cancer[J].Anticancer Res,2013,33(6):2561-2567.
[15] Hou T,Ou J,Zhao X,et al.MicroRNA-196a promotes cervical cancer proliferation through the regulation of FOXO1 and p27Kip1[J].Br J Cancer,2014,110(5):1260-1268.
[16] Zhang J,Zheng F,Yu G,et al.miR-196a targets netrin 4 and regulates cell proliferation and migration of cervical cancer cells[J].Biochem Biophys Res Commun,2013,440(4):582-588.
[17] Tsai MM,Wang CS,Tsai CY,et al.MicroRNA-196a/-196b promote cell metastasis via negative regulation of radixin in human gastric cancer[J].Cancer Lett,2014,351(2):222-231.
[18] Wang K,Li J,Guo H,et al.MiR-196a binding-site SNP regulates RAP1A expression contributing to esophageal squamous cell carcinoma risk and metastasis[J].Carcinogenesis,2012,33(11):2147-2154.
[19] Yang W,Hong L.LncRNA GAS5 suppresses the tumorigenesis of cervical cancer by downregulating miR-196a and miR-205[J].Tumour Biol,2017,39(7):1010428317711315.
[2] 孙娟娟,宋晓婕,罗自娟,等.高危型HPV筛查与TCT联合检查在宫颈癌筛查中的临床应用[J].现代生物医学进展,2016,16(12):2336-2338,2263.
[3] Hayes J,Peruzzi PP,Lawler S.MicroRNAs in cancer:biomarkers,functions and therapy[J].Trends Mol Med,2014,20(8):460-469.
[4] Chen TH,Lee C,Chiu CT,et al.Circulating microRNA-196a is an early gastric cancer biomarker[J].Oncotarget,2017,9(12):10317-10323.
[5] Yang L,Peng F,Qin J,et al.Downregulation of microRNA-196a inhibits human liver cancer cell proliferation and invasion by targeting FOXO1[J].Oncol Rep,2017,38(4):2148-2154.
[6] Chen H,Fan Y,Xu W,et al.Exploration of miR-1202 and miR-196a in human endometrial cancer based on high throughout gene screening analysis[J].Oncol Rep,2017,37(6):3493-3501.
[7] Xu YF,Hannafon BN,Zhao YD,et al.Plasma exosome miR-196a and miR-1246 are potential indicators of localized pancreatic cancer[J].Oncotarget,2017,8(44):77028-77040.
[8] Liu P,Xin F,Ma CF.Clinical significance of serum miR-196a in cervical intraepithelial neoplasia and cervical cancer[J].Genet Mol Res,2015,14(4):17995-18002.
[9] 李慧聪.miR-196a高表达在肾脏损伤中作用的分子生物学机制[J].中国老年学杂志,2018,38(10):2472-2475.
[10] Yang B,Li SZ,Ma L,et al.Expression and mechanism of action of miR-196a in epithelial ovarian cancer[J].Asian Pac J Trop Med,2016,9(11):1105-1110.
[11] Jiang CF,Shi ZM,Li DM,et al.Estrogen-induced miR-196a elevation promotes tumor growth and metastasis via targeting SPRED1 in breast cancer[J].Mol Cancer,2018,17(1):83.
[12] Lu YC,Chang JT,Huang YC,et al.Combined determination of circulating miR-196a and miR-196b levels produces high sensitivity and specificity for early detection of oral cancer[J].Clin Biochem,2015,48(3):115-121.
[13] Zhang C,Yao C,Li H,et al.Combined elevation of microRNA-196a and microRNA-196b in sera predicts unfavorable prognosis in patients with osteosarcomas[J].Int J Mol Sci,2014,15(4):6544-6555.
[14] Gocze K,Gombos K,Juhasz K,et al.Unique microRNA expression profiles in cervical cancer[J].Anticancer Res,2013,33(6):2561-2567.
[15] Hou T,Ou J,Zhao X,et al.MicroRNA-196a promotes cervical cancer proliferation through the regulation of FOXO1 and p27Kip1[J].Br J Cancer,2014,110(5):1260-1268.
[16] Zhang J,Zheng F,Yu G,et al.miR-196a targets netrin 4 and regulates cell proliferation and migration of cervical cancer cells[J].Biochem Biophys Res Commun,2013,440(4):582-588.
[17] Tsai MM,Wang CS,Tsai CY,et al.MicroRNA-196a/-196b promote cell metastasis via negative regulation of radixin in human gastric cancer[J].Cancer Lett,2014,351(2):222-231.
[18] Wang K,Li J,Guo H,et al.MiR-196a binding-site SNP regulates RAP1A expression contributing to esophageal squamous cell carcinoma risk and metastasis[J].Carcinogenesis,2012,33(11):2147-2154.
[19] Yang W,Hong L.LncRNA GAS5 suppresses the tumorigenesis of cervical cancer by downregulating miR-196a and miR-205[J].Tumour Biol,2017,39(7):1010428317711315.