干旱胁迫与温度变化对粉花桔梗种子萌发特性的影响
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摘要
旨在为粉花桔梗种子萌发提供技术支撑,为桔梗新品系的示范推广提供技术参考。以粉花桔梗种子为试验材料,设置4个PEG-6000溶液处理[5%PEG-6000(H1)、10%PEG-6000(H2)、15%PEG-6000(H3)和蒸馏水对照(CK)]和3个温度处理[15℃(T1)、20℃(T2)、25℃(T3)],进行光照培养(12 h光/12 h暗),测定种子发芽率和发芽势。结果表明,不同干旱胁迫程度(PEG浓度)与温度变化对粉花桔梗种子萌发的时间、发芽率以及发芽势均呈现出显著性相关。在不同温度条件下,随着干旱程度的增加,发芽率逐渐的降低;同时低温不利于桔梗种子萌发,当温度低于15℃或干旱程度程度大于15%PEG时,种子萌发起始时间延迟,发芽率开始降低。在干旱胁迫程度小于5%PEG的条件下,温度在20℃、25℃时的条件下桔梗种子能够良好萌发。
The objectives are to provide the technical support for seed germination of Platycodon Grandiflorum,and technical reference for demonstration and popularization of new lines of Platycodon Grandiflorum.The seeds of Platycodon Grandiflorum were used as materials,four PEG-6000 treatments[5%PEG-6000(H1),10%PEG-6000(H2),15%PEG-6000(H3)and distilled water(CK)]and three temperature treatments[15℃(T1),20℃(T2),25℃(T3)]were set up.The seeds were cultivated with light culture(12 h light/12 h dark),and then the germination rate and germination potential was detected.The results showed that different drought stresses(PEG concentration)and temperature change affected the seed germination time,germination rate and germination potential significantly.Under different temperature,the germination rate decreased gradually with the drought stress increase.Low temperature was bad for the seed germination,the initiatory time of seed germination was delayed and the germination rate began to decrease when the temperature was below 15℃,or the degree of drought was more than 15%PEG.When the drought stress was below 5%PEG,the germination of Platycodon grandiflorum was good at the temperature of 20℃ and 25℃.
The objectives are to provide the technical support for seed germination of Platycodon Grandiflorum,and technical reference for demonstration and popularization of new lines of Platycodon Grandiflorum.The seeds of Platycodon Grandiflorum were used as materials,four PEG-6000 treatments[5%PEG-6000(H1),10%PEG-6000(H2),15%PEG-6000(H3)and distilled water(CK)]and three temperature treatments[15℃(T1),20℃(T2),25℃(T3)]were set up.The seeds were cultivated with light culture(12 h light/12 h dark),and then the germination rate and germination potential was detected.The results showed that different drought stresses(PEG concentration)and temperature change affected the seed germination time,germination rate and germination potential significantly.Under different temperature,the germination rate decreased gradually with the drought stress increase.Low temperature was bad for the seed germination,the initiatory time of seed germination was delayed and the germination rate began to decrease when the temperature was below 15℃,or the degree of drought was more than 15%PEG.When the drought stress was below 5%PEG,the germination of Platycodon grandiflorum was good at the temperature of 20℃ and 25℃.
引文
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[2]仉劲,李国清,毕研文,等.不同桔梗品种有效成分比较分析[J].时珍国医国药,2016(12):3010-3012.
[3]李国清,毕研文,陈宝芳,等.中草药桔梗人工栽培研究进展[J].农学学报,2016(07):55-59.
[4]仉劲,李国清,毕研文,等.中药材桔梗中常量元素和微量元素含量分析[J].农学学报,2017,7(07):43-46.
[5]陈宝芳,仉劲,毕研文,等.粉花桔梗与紫花桔梗中氨基酸组成与含量的比较[J].农业科技通讯,2016(06):140-142.
[6]庞立杰,王彦,许永华,等.桔梗的药用价值及其栽培[J].人参研究,2003(4):38-39.
[7]郭巧生.药用植物栽培学[M].北京:高等教育出版社,2004.
[8]刘自刚,张雁,王新军.桔梗育种研究进展[J].中草药,2006,37(6):7-8.
[9]严一字,吴基日.桔梗种子质量对发芽、出苗及苗素质的影响[J].中草药,2007,389(11):1726-1729.
[10]谭玲玲,胡正海.桔梗根的发育解剖学研究[J].西北植物学报,2014,34(5):938-942.
[11]刘自刚.桔梗种子休眠解除方法研究[J].种子,2009,28(1):72-74.
[12]张鸿雁,胡晓黎,李会军,等.桔梗适宜气候及优质高产栽培技术[J].陕西农业科学,2011,57(02):255-257.
[13]李尚擘.皖桔梗生长与田间环境因子的相关性研究[D].合肥:安徽大学,2015.
[14]王进,陈叶,肖占文.光照、温度和土壤水分对孜然芹种子萌发和幼苗生长的影响[J].植物生理学通讯,2006,42(6):1106-1108.
[15]史薇,徐海量,赵新风,等.胀果甘草种子萌发对干旱胁迫的生理响[J].应生态学报,2010,30(8):2112-2117.
[16]马红媛,梁正伟,孔祥军,等.盐分、温度及其互作对羊草种子发芽率和幼苗生长的影响[J].生态学报,2008,28(10):4710-4717.
[17]马红媛,梁正伟.不同p H值土壤及其浸提液对羊草种子萌发和幼苗生长的影响[J].植物学通报,2007,24(2):181-188.
[18]郭巧生,赵荣梅,刘丽,等.桔梗种子发芽特性的研究[J].中国中药杂志,2006,31(1):870-881.
[19]刘自刚,沈冰,张雁.桔梗种子萌发对低温、干旱及互作胁迫的响[J].应生态学报,2013,33(8):2615-2622.
[20]高留喜.山东春季降水特征与成因分析[D].青岛:中国海洋大学,2003.
[21]Pei L X,Bao Y W,Ma L,et al.A Sensitive Method for Determination of Platycodin D in Rat Plasma Using Liquid Chromatography/Tandem Mass Spectrometry and its Application to a Pharmacokinetic Study[J].Planta Medica,2012,78(03):244-251.
[22]高留喜,刘秦玉.山东春季降水的时空变化特征分析[J].高原气象,2005(05):811-815.
[23]韩荣青,包维虎,赵明华,等.1961-2012年山东省气温变化及对农业热量条件影响[J].中国农学通报,2017(16):112-122.
[24]田甜.桔梗种子萌发生理生态特性研究[D].长春:吉林农业大学,2008.
[25]丁膺宾,张海艳.PEG模拟干旱对桔梗种子发芽和幼苗生长的影响[J].山东农业科学,2016,48(10):51-53,57.