可溶性微生物产物对偶氮染料废水脱色及产电性能的影响
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摘要
可溶性微生物产物(Soluble Microbial Product,SMP)是活性污泥污水处理体系中的微生物的重要代谢产物,是污泥絮体形成以及保证体系稳定运行的关键因素之一.本文对CASS和A2O工艺产生的脱水污泥进行了好氧和厌氧培养,提取了SMP和胞外聚合物(Extracellular Polymeric Substances,EPS),表征并探究了两者对含偶氮染料废水的微生物燃料电池产电性能的影响,以及对脱色效率的改善情况.红外分析表明,不同工艺和培养方法得到的SMP和EPS的成分构成相似度很高;PACl沉降试验表明,SMP中的大分子物质含量普遍低于EPS.SMP和EPS均可提升微生物燃料电池的电压,最高可达130mV,且两者均可提高偶氮染料的脱色率,最高可达93%.这些研究表明SMP和EPS有作为染料废水产电资源化助剂的价值.
Soluble microbial product(SMP),being an important metabolite of the microbes in activated sludge system of wastewater treatment,plays an essential role in the formation of sludge flocs and the stable operation of treatment system.The characteristics of SMP and extracellular polymeric substances(EPS)from the sludge of Cyclic Activated Sludge System(CASS)and Anaeroxic-Anoxic-Oxic(A2O)treatment processes,cultured anaerobically and aerobically,were investigated after long-term(60days)cultivation and used for the enhancement of the electricity generation and decoloring rate of the microbial fuel cell(MFC)to treat azodye-containing wastewater.The results showed that the SMP and EPS from different processes and cultivating methods were of high similarity in composition while the EPS had higher content of macromolecular substances than that in SMP according to the data of PACL sedimentation test.Both SMP and EPS were confirmed to be effective in enhancing the voltage to its peak value of 130 mV and the decoloring rate to 93%of the MFC for azodye-containing wastewater.The present study suggests that EPS and SMP are of valuable as auxiliary in the electricity reclamation from azodye-containing wastewater treatment.
Soluble microbial product(SMP),being an important metabolite of the microbes in activated sludge system of wastewater treatment,plays an essential role in the formation of sludge flocs and the stable operation of treatment system.The characteristics of SMP and extracellular polymeric substances(EPS)from the sludge of Cyclic Activated Sludge System(CASS)and Anaeroxic-Anoxic-Oxic(A2O)treatment processes,cultured anaerobically and aerobically,were investigated after long-term(60days)cultivation and used for the enhancement of the electricity generation and decoloring rate of the microbial fuel cell(MFC)to treat azodye-containing wastewater.The results showed that the SMP and EPS from different processes and cultivating methods were of high similarity in composition while the EPS had higher content of macromolecular substances than that in SMP according to the data of PACL sedimentation test.Both SMP and EPS were confirmed to be effective in enhancing the voltage to its peak value of 130 mV and the decoloring rate to 93%of the MFC for azodye-containing wastewater.The present study suggests that EPS and SMP are of valuable as auxiliary in the electricity reclamation from azodye-containing wastewater treatment.
引文
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[15]Laspidou C.S.,Rittmann B.E..Non-steady state modeling of extracellular polymeric substances,soluble microbial products and active and inert biomass[J].Water Res.,2002,36(8):1 983-1 992.
[16]Chen L.,Tian Y.,Cao C.Q.,et al.Interaction energy evaluation of soluble microbial products(SMP)on different membrane surfaces:role of the reconstructed membrane topology[J].Water Res,2012,46(8):2 693-2 704.
[17]Chang J.S.,Chen B.Y.,Lin Y.C..Stimulation of bacterial decolorization of an azo dye by extracellular metabolites from Escherichia coli strain NO3[J].Bioresour.Technol.,2004,91(3):243-248.
[18]Oh S.,Logan B.E..Proton exchange membrane and electrode surface areas as factors that affect power generation in microbial fuel cells[J].Appl.Microbiol.Biotechnol.,2006,70(2):162-169.
[19]Liang D,Wei J.,Song Y.H.,et al.The characteristics of extracellular polymeric substances and soluble microbial products in moving bed biofilm reactor-membrane bioreactor[J].Bioresour.Technol,2013,148(9):436-442.
[20]A.Ramesh,Duu Jong,Lee S.G.Hong.Soluble microbial products(SMP)and souble extracellular polymeric substances(EPS)from wastewater sludge[J].Appl.Microbiol and Biotechnol,2006,73(1):219-225.
[21]Bruce E.Logan.微生物燃料电池[M].冯玉杰,王鑫.北京:化学工业出版社,2009:18-30.
[2]孟凡松,冯雷雨.污水厌氧处理系统中可溶性微生物产物的研究进展[J].工业水处理,2012,32(6):6-10.
[3]孔晓英,李连华,孙永明.微生物燃料电池产能原理及输出功率的影响因素[J].现代化工,2007,27(2):282-286.
[4]Barker D.J.,Stuckey D.C..A review of soluble microbial products(SMP)in wastewater treatment systems[J].Water Res.,1999,33(14):3 063-3 082.
[5]Yao M,Ladewig B,Zhang K.Identification of the change of solublemicrobial products on membrane fouling in membrane bioreactor(MBR)[J].Desalination,2011,278(1):126-131.
[6]Berk R.S.,Canfield J.H..Bioelectrochemical energy conversion[J].Appl.Microbiol,1964,12(1):10-12.
[7]Rao J.R.,Richter G.J.,Von Sturm F.,et al.The performance of glucose electrodes and the haracteristics of different biofuel cell constructions[J].Bioelectrochem.Bioenerg,1976,3(1):139-150.
[8]Loqan B.E.,Hamelers B.,Rozendal R.,et al.Microbial fuel cells:Methodology and technology[J].Environ.Sci.Technol,2006,40(17):5 181-5 192.
[9]Mei X.J.,Wang Z.W.,Zheng X..Soluble microbial products in membrane bioreactors in the presence of ZnO nanoparticles[J].J.Membr.Sci.,2014,451(1):169-176.
[10]Lefebvre O.,Tan Z.,Shen Y.J..Optimization of a microbial fuel cell for wastewater treatment using recycled scrap metals as a cost-effective cathode material[J].Bioresour Technol,2013,127:158-64.
[11]Tommasi T.,Chiolerio A.,Crepaldi M..A microbial fuel cell powering an all-digital piezoresistive wireless sensor system[J].Microl.Technol.,2013,20(5):1 023-1033.
[12]Jiang T.,Myngheer S.,Pauw D.J.W.,et al.Modelling the production and degradation of soluble microbial products(SMP)in membrane bioreactors(MBR)[J].Water Res.,2008,42(20):4 955-4 964.
[13]Largus T.A.,Khursheed K.,Muthanna H.A.,et al.Production of bioenergy and biochemicals from industrial and agricul tural waste[J].Trends Biotechnol.,2004,22(9):477-485.
[14]Ibeid S.,Elektorowicz M.,Oleszkiewicz J.A..Impact of electro-coagulation on the fate of soluble microbial products(SMP)in submerged membrane electro-bioreactor(SMEBR)[J].Annual Conference-Canadian Society for Civil Engineering(CSCE),2010,1:634-640.
[15]Laspidou C.S.,Rittmann B.E..Non-steady state modeling of extracellular polymeric substances,soluble microbial products and active and inert biomass[J].Water Res.,2002,36(8):1 983-1 992.
[16]Chen L.,Tian Y.,Cao C.Q.,et al.Interaction energy evaluation of soluble microbial products(SMP)on different membrane surfaces:role of the reconstructed membrane topology[J].Water Res,2012,46(8):2 693-2 704.
[17]Chang J.S.,Chen B.Y.,Lin Y.C..Stimulation of bacterial decolorization of an azo dye by extracellular metabolites from Escherichia coli strain NO3[J].Bioresour.Technol.,2004,91(3):243-248.
[18]Oh S.,Logan B.E..Proton exchange membrane and electrode surface areas as factors that affect power generation in microbial fuel cells[J].Appl.Microbiol.Biotechnol.,2006,70(2):162-169.
[19]Liang D,Wei J.,Song Y.H.,et al.The characteristics of extracellular polymeric substances and soluble microbial products in moving bed biofilm reactor-membrane bioreactor[J].Bioresour.Technol,2013,148(9):436-442.
[20]A.Ramesh,Duu Jong,Lee S.G.Hong.Soluble microbial products(SMP)and souble extracellular polymeric substances(EPS)from wastewater sludge[J].Appl.Microbiol and Biotechnol,2006,73(1):219-225.
[21]Bruce E.Logan.微生物燃料电池[M].冯玉杰,王鑫.北京:化学工业出版社,2009:18-30.