人参、黄芪药对血中移行成分质谱分析
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摘要
目的:探讨人参、黄芪药对在正常大鼠血中移行成分。方法:利用UPLC-MS/MS联用方法对人参、黄芪药对体外样品及灌胃后正常大鼠血清样品进行分析,比较各样品分析结果,确认人参、黄芪药对的血中移行成分及其药材饮片来源。结果:推断出给药后大鼠血清中原型成分11个,其中来源于人参的化学成分8个,分别是人参皂苷Ra1、人参皂苷Ra3、人参皂苷Rb1、人参皂苷Rc、人参皂苷Re、人参皂苷Rg2、人参皂苷Rg5、三七皂苷Fe;来源于黄芪的化学成分3个,分别是毛蕊异黄酮葡萄糖苷、芒柄花素、山柰酚苷。结论:初步确立了人参、黄芪的血中移行成分,为阐明该药对血清药物化学成分及药效物质基础研究提供了参考。
Objective To investigate the migration components of Ginseng and Astragalus membranaceus in normal rat blood. Methods: UPLC-MS/MS was used to analyze the serum samples of Ginseng and Astragalus membranaceus medicinal in vitro samples and normal rat serum sample after intragastric administration. The results of each sample were compared to confirm the blood migration components of Ginseng and Astragalus membranaceus and the sources of the medicinal herbs. Results: Eleven of the prototype components in the serum of the rats were inferred. Among them,there were 8 chemical components derived from Ginseng,namely ginsenoside Ra1,ginsenoside Ra3,ginsenoside Rb1,ginsenoside Rc,ginsenoside Re and ginsenoside. Rg2,ginsenoside Rg5,notoginsenoside Fe; there are three chemical components derived from Astragalus membranaceus,respectively,they are flavonoid glycosides,formononetin,and kaempferol. Conclusion: The blood migration components of Ginseng and Astragalus membranaceus were preliminarily established,which would provide a reference for the basic research of serum medicinal components and pharmacodynamic substances.
Objective To investigate the migration components of Ginseng and Astragalus membranaceus in normal rat blood. Methods: UPLC-MS/MS was used to analyze the serum samples of Ginseng and Astragalus membranaceus medicinal in vitro samples and normal rat serum sample after intragastric administration. The results of each sample were compared to confirm the blood migration components of Ginseng and Astragalus membranaceus and the sources of the medicinal herbs. Results: Eleven of the prototype components in the serum of the rats were inferred. Among them,there were 8 chemical components derived from Ginseng,namely ginsenoside Ra1,ginsenoside Ra3,ginsenoside Rb1,ginsenoside Rc,ginsenoside Re and ginsenoside. Rg2,ginsenoside Rg5,notoginsenoside Fe; there are three chemical components derived from Astragalus membranaceus,respectively,they are flavonoid glycosides,formononetin,and kaempferol. Conclusion: The blood migration components of Ginseng and Astragalus membranaceus were preliminarily established,which would provide a reference for the basic research of serum medicinal components and pharmacodynamic substances.
引文
[1]王喜军.中药血清药物化学的研究动态及发展趋势[J].中国中药杂志,2006,31(10):789-792.
[2]常存库,王喜军.中药血清药物化学理论与方法的意义[J].世界科学技术——中医药现代化,2010,12(4):634-637.
[3]黄财顺,向诚,李宝才,等.基于中药血清药物化学的活性成分筛选的现状和问题[J].中草药,2014,45(20):3009-3014.
[4]闫广利,孙晖,张爱华,等.中药血清药物化学研究概况及其理论和方法拓展[J].中国中药杂志,2015,40(17):3406-3412.
[5]张灵娜,林兵,宋洪涛,等.中药血清药理学、血清药物化学的研究概况及展望[J].中草药,2015,46(17):2662-2666.
[6]李凤林.人参茎叶皂苷药理作用的研究进展[J].贵州农业科学,2013,41(2):54-57.
[7]冯彦.人参药理作用及临床应用研究进展[J].中医临床研究,2013,5(6):121-122.
[8]周承.中药黄芪药理作用及临床应用研究[J].亚太传统医药,2014,10(22):100-101.
[9]孙政华,邵晶,郭玫.黄芪化学成分及药理作用研究进展[J].中医临床研究,2015,7(25):22-25.
[10]刘月,初杰.人参黄芪的性味与配伍对方剂构成的影响[J].辽宁中医药大学学报,2008,10(50):5.
[11]李雪峰,徐振秋,闫明,等.效应面法优化芪白平肺颗粒中人参黄芪浸膏真空带式干燥工艺[J].中国中药杂志,2015,40(4):3987-3992.
[12]GUAN S,XU W,HAN F,GU W,et al. Ginsenoside Rg1Attenuates Cigarette Smoke-Induced Pulmonary Epithelial-Mesenchymal Transition via Inhibition of the TGF-β1/Smad Pathway[J]. Biomed Res Int,2017,7171404.
[13]SHERGIS J L,DI Y M,ZHANG A L,et al. Therapeutic potential of Panax ginseng and ginsenosides in the treatment of chronic obstructive pulmonary disease[J]. Complement Ther Med,2014,22(5):944-953.
[14]SONG J,LI J,ZHENG S R,et al. Anti-inflammatory and immunoregulatory effects of Yupingfeng powder on chronic bronchitis rats[J]. Chin J Integr Med,2013,19(5):353-359.
[15]ZHANG Z,DAI X,QI J,et al. Astragalus mongholicus(Fisch.)Bge Improves Peripheral Treg Cell Immunity Imbalance in the Children With Viral Myocarditis by Reducing the Levels of miR-146b and miR-155[J]. Front Pediatr,2018,6:139.
[16]PENG Z,LI JI S,TIAN Y,et al. Restoring Th17/Treg balance via modulation of STAT3 and STAT5 activation contributes to the amelioration of chronic obstructive pulmonary disease by Bufei Yishen formula[J]. J of Ethnoph,2018,217,152-162.
[2]常存库,王喜军.中药血清药物化学理论与方法的意义[J].世界科学技术——中医药现代化,2010,12(4):634-637.
[3]黄财顺,向诚,李宝才,等.基于中药血清药物化学的活性成分筛选的现状和问题[J].中草药,2014,45(20):3009-3014.
[4]闫广利,孙晖,张爱华,等.中药血清药物化学研究概况及其理论和方法拓展[J].中国中药杂志,2015,40(17):3406-3412.
[5]张灵娜,林兵,宋洪涛,等.中药血清药理学、血清药物化学的研究概况及展望[J].中草药,2015,46(17):2662-2666.
[6]李凤林.人参茎叶皂苷药理作用的研究进展[J].贵州农业科学,2013,41(2):54-57.
[7]冯彦.人参药理作用及临床应用研究进展[J].中医临床研究,2013,5(6):121-122.
[8]周承.中药黄芪药理作用及临床应用研究[J].亚太传统医药,2014,10(22):100-101.
[9]孙政华,邵晶,郭玫.黄芪化学成分及药理作用研究进展[J].中医临床研究,2015,7(25):22-25.
[10]刘月,初杰.人参黄芪的性味与配伍对方剂构成的影响[J].辽宁中医药大学学报,2008,10(50):5.
[11]李雪峰,徐振秋,闫明,等.效应面法优化芪白平肺颗粒中人参黄芪浸膏真空带式干燥工艺[J].中国中药杂志,2015,40(4):3987-3992.
[12]GUAN S,XU W,HAN F,GU W,et al. Ginsenoside Rg1Attenuates Cigarette Smoke-Induced Pulmonary Epithelial-Mesenchymal Transition via Inhibition of the TGF-β1/Smad Pathway[J]. Biomed Res Int,2017,7171404.
[13]SHERGIS J L,DI Y M,ZHANG A L,et al. Therapeutic potential of Panax ginseng and ginsenosides in the treatment of chronic obstructive pulmonary disease[J]. Complement Ther Med,2014,22(5):944-953.
[14]SONG J,LI J,ZHENG S R,et al. Anti-inflammatory and immunoregulatory effects of Yupingfeng powder on chronic bronchitis rats[J]. Chin J Integr Med,2013,19(5):353-359.
[15]ZHANG Z,DAI X,QI J,et al. Astragalus mongholicus(Fisch.)Bge Improves Peripheral Treg Cell Immunity Imbalance in the Children With Viral Myocarditis by Reducing the Levels of miR-146b and miR-155[J]. Front Pediatr,2018,6:139.
[16]PENG Z,LI JI S,TIAN Y,et al. Restoring Th17/Treg balance via modulation of STAT3 and STAT5 activation contributes to the amelioration of chronic obstructive pulmonary disease by Bufei Yishen formula[J]. J of Ethnoph,2018,217,152-162.