美洲大蠊提取液对溃疡性结肠炎大鼠EGF,IGF-1表达的影响
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摘要
目的观察美洲大蠊提取液(ACE)对大鼠溃疡性结肠炎(UC)的治疗效果及对大鼠结肠黏膜表皮生长因子(EGF)、胰岛素样生长因子-1(IGF-1)表达的影响,并与地塞米松作比较。方法用2,4,6-三硝基苯磺酸(TNBS)灌肠制作大鼠UC模型,取40只Sprague Dawley大鼠,随机均分为ACE组、地塞米松组、模型对照组、正常对照组4组,每组10只。造模并干预性治疗10d后观察每组大鼠疾病活动指数(DAI)及肠黏膜损伤指数评分(CMDI),并检测结肠黏膜组织中EGF及IGF-1的表达水平。结果与模型对照组相比,ACE及地塞米松组DAI评分及CMDI评分显著降低(P<0.01);与地塞米松组相比,ACE组DAI评分较高(P<0.05),而CMDI评分差距无统计学意义(P>0.05)。与模型对照组对比,ACE组及地塞米松组的EGF表达水平升高(P<0.05),ACE组与地塞米松组IGF-1表达水平均降低(P<0.05)。结论 ACE对TNBS诱导的大鼠UC有治疗作用,其作用较地塞米松弱,上调EGF及下调IGF-1的表达,可能为其治疗机制之一。
Objective To observe the therapeutic effects and the expression of Epidermal Growth Factor(EGF) and Insulin-like Growth Factors-1(IGF-1) of America Cockroach Extract(ACE) on ulcerative colitis of rats,and to compare the effects of ACE on UC with Dexamethasone.Methods Forty healthy Sprague-Dawley rats were randomly divided into normal control group,model control group,ACE group and Dexamethasone group,with 10 rats in each group.Colitis was induced by rectal administration of TNBS in the model control group,ACE group and Dexamethasone group.Rats in the ACE group were treated with ACE,and the Dexamethasone group were treated with Dexamethasone.Disease Activity Index(DAI) and Colon Macroscopic Damage Index(CMDI) were calculated,and the expressions of EGF and IGF-1 in colon tissues were detected.Results Compared with the model control,the DAI and CMDI scores in ACE and Dexamethasone group were decreased significantly(P < 0.01).The EGF expression level in ACE group were increased(P < 0.05),the IGF-1 expression level in ACE group and Dexamethasone group were decreased(P <0.05).Conclusion ACE has therapeutic effect for the UC of rats,but its effect is relatively weak when compared with dexamethasone.The increased expression of EGF and decreased expression of IGF-1 maybe one of the possible mechanisms.
Objective To observe the therapeutic effects and the expression of Epidermal Growth Factor(EGF) and Insulin-like Growth Factors-1(IGF-1) of America Cockroach Extract(ACE) on ulcerative colitis of rats,and to compare the effects of ACE on UC with Dexamethasone.Methods Forty healthy Sprague-Dawley rats were randomly divided into normal control group,model control group,ACE group and Dexamethasone group,with 10 rats in each group.Colitis was induced by rectal administration of TNBS in the model control group,ACE group and Dexamethasone group.Rats in the ACE group were treated with ACE,and the Dexamethasone group were treated with Dexamethasone.Disease Activity Index(DAI) and Colon Macroscopic Damage Index(CMDI) were calculated,and the expressions of EGF and IGF-1 in colon tissues were detected.Results Compared with the model control,the DAI and CMDI scores in ACE and Dexamethasone group were decreased significantly(P < 0.01).The EGF expression level in ACE group were increased(P < 0.05),the IGF-1 expression level in ACE group and Dexamethasone group were decreased(P <0.05).Conclusion ACE has therapeutic effect for the UC of rats,but its effect is relatively weak when compared with dexamethasone.The increased expression of EGF and decreased expression of IGF-1 maybe one of the possible mechanisms.
引文
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[2]何小姣,奚再兴,原皓.美洲大蠊提取液对溃疡性结肠炎大鼠模型结肠黏膜MUC2,IL-6表达的影响[J].潍坊医学院学报,2012,34(1):59~62.
[3]张涛,谢建军.大鼠溃疡性结肠炎模型的实验研究[J].中国中西医结合消化杂志,2006,14(4):240~242.
[4]陈迟,冉志华,萧树东,等.普伐他汀对乙酸诱导大鼠结肠炎的治疗作用及其机制的研究[J].中华医学杂志,2006,86(18):1284~1288.
[5]王皓,欧阳钦,胡仁伟.三硝基苯磺酸结肠炎动物模型的建立[J].胃肠病学,2001,6(1):7~10.
[6]龚励军,李永祥,汪国华.康复新液治疗复发性口腔溃疡82例观察[J].浙江中医杂志,2009,44(10):779.
[7]Rahman FB.Heparin-binding EGF-like factor augments esophageal epithelial cell proliferation,migration and inhibits TRAlL-mediated apoptosis via EGFR/MAPK signaling[J].Scand J Gastroenterol,2010,45(11):1350~1359.
[8]Kurmasheva RT,Houghton PJ.IGF-1 mediated survival pathways in normal and malignant cells[J].Biochim Biophys Acta,2006,1766(1):1~22.
[9]Lee YJ.Adenovirus expressing shRNA to IGF-1R enhances the chemosensitivity of lung cancer cell lines by blocking IGF-1 pathway[J].Lung Cancer,2007,55(3):279~286.
[10]王映珍,王世文,李培杰,等.胰岛素样生长因子-Ⅰ对重症急性胰腺炎大鼠小肠黏膜上皮细胞bax和bcl-2mRNA表达的影响[J].世界华人消化杂志,2008,16(13):1396~1401.
[11]任燕华,潘励山,宁亚清,等.康复新胶囊对动物的脱毒药效学评价[J].中国药物滥用防治杂志,2000,4(27):42~46.