康媛颗粒HPLC指纹图谱的建立及主要成分含量测定
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摘要
目的建立康媛颗粒HPLC指纹图谱,同时检测芍药苷、阿魏酸、甘草苷、橙皮苷4个成分的含量。方法采用YMC-Pack-ODS-A C18色谱柱(250 mm×4.6 mm,5μm),流动相为0.05%磷酸水-乙腈,梯度洗脱,流速0.6 mL/min,柱温30℃。结果 10批康媛颗粒的指纹图谱相似度较高,确定9个共有色谱峰。芍药苷、阿魏酸、甘草苷、陈皮苷含量分别在1.102～11.02μg(r=1)、0.201～2.01μg(r=0.999 5)、0.270～2.70μg(r=0.9998)、0.102～1.02μg(r=1)范围内与峰面积呈良好的线性关系,仪器精密度良好,供试品溶液在24h内基本稳定。结论本研究建立的康媛颗粒指纹图谱及4个成分含量测定方法简便、稳定、准确可靠,可用于康媛颗粒的质量控制。
Objective To establish HPLC fingerprints of Kangyuan Granules and determine the contents of peoniflorin, ferulic acid, glycyrrhizin and hesperidin. Methods The samples were separated by the chromatographic column of YMC-Pack-ODS-A C18(250 mm × 4.6 mm, 5 μm). The mobile phase was acetonitrile-0.05% phosphoric acid aqueous solution and gradient elution. The flow rate was 0.6 mL/min, and the column temperature was at 30 ℃. Results The fingerprints of 10 batches of Kangyuan Granules had higher similarity and nine common chromatogram peaks were determined. The contents of peoniflorin, ferulic acid, glycyrrhizin and hesperidin were among the ranges of 1.102–11.02 μg(r=1), 0.201–2.01 μg(r=0.999 5), 0.270–2.70 μg(r=0.999 8) and 0.102–1.02 μg(r=1), respectively, which showed good linear relationship with the peak area. The instrument precision was good and the sample solution was basically stable within 24 hours. Conclusion The method of Kangyuan Granules fingerprints and the content determination of four components are simple, stable, accurate and reliable, which can be used for the quality control of Kangyuan Granules.
Objective To establish HPLC fingerprints of Kangyuan Granules and determine the contents of peoniflorin, ferulic acid, glycyrrhizin and hesperidin. Methods The samples were separated by the chromatographic column of YMC-Pack-ODS-A C18(250 mm × 4.6 mm, 5 μm). The mobile phase was acetonitrile-0.05% phosphoric acid aqueous solution and gradient elution. The flow rate was 0.6 mL/min, and the column temperature was at 30 ℃. Results The fingerprints of 10 batches of Kangyuan Granules had higher similarity and nine common chromatogram peaks were determined. The contents of peoniflorin, ferulic acid, glycyrrhizin and hesperidin were among the ranges of 1.102–11.02 μg(r=1), 0.201–2.01 μg(r=0.999 5), 0.270–2.70 μg(r=0.999 8) and 0.102–1.02 μg(r=1), respectively, which showed good linear relationship with the peak area. The instrument precision was good and the sample solution was basically stable within 24 hours. Conclusion The method of Kangyuan Granules fingerprints and the content determination of four components are simple, stable, accurate and reliable, which can be used for the quality control of Kangyuan Granules.
引文
[1]张礼菊.色谱指纹图谱在中药质量标准体系中的应用[J].安徽医药,2009,13(11):1414-1417.
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[4]潘萍,潘金火.康媛颗粒质量标准研究[J].中国中医药信息杂志,2007,14(10):42-44.
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[6]刘仲.HPLC法测定康媛颗粒中芍药苷的含量[J].安徽医药,2005,9(1):41-42.
[7]孙立华,吴爱英,张春辉.高效液相色谱法测定康媛颗粒中阿魏酸的含量[J].中国药事,2003,17(12):36-37.
[8]李辉.康媛颗粒质量标准研究[J].黑龙江医药,2005,18(4):257-259.
[2]刘利军,朱又红,龚云.康媛颗粒质量标准研究[J].海峡药学,2009,21(9):50-52.
[3]朱玉华,种衍芝,吴敬超,等.高效液相色谱法测定康媛颗粒中芍药苷的含量[J].山东医药工业,2002,21(4):19-20.
[4]潘萍,潘金火.康媛颗粒质量标准研究[J].中国中医药信息杂志,2007,14(10):42-44.
[5]孙立华,吴爱英,张春辉.康媛颗粒质量标准的研究[J].中国药品标准,2006,7(1):66-68,42.
[6]刘仲.HPLC法测定康媛颗粒中芍药苷的含量[J].安徽医药,2005,9(1):41-42.
[7]孙立华,吴爱英,张春辉.高效液相色谱法测定康媛颗粒中阿魏酸的含量[J].中国药事,2003,17(12):36-37.
[8]李辉.康媛颗粒质量标准研究[J].黑龙江医药,2005,18(4):257-259.