LC3在成釉细胞瘤中的表达及意义
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摘要
目的探讨自噬标记蛋白微管相关蛋白1轻链3 (LC3)在成釉细胞瘤组织中的表达及意义。方法应用免疫组织化学染色方法检测104例成釉细胞瘤组织及20例正常口腔黏膜组织中LC3的表达,通过半定量方法分析其表达与临床病理资料的关系。结果 LC3在成釉细胞瘤上皮细胞中的阳性表达率显著高于正常口腔黏膜组织,差异有统计学意义(P <0.05);LC3的表达在性别、年龄、是否复发方面的差异无统计学意义(P> 0.05);发生于下颌骨的成釉细胞瘤中LC3的阳性表达率高于发生于上颌骨及牙龈的成釉细胞瘤,差异有统计学意义(P <0.05);实性/多囊型中LC3的阳性表达率显著高于其他3种病理分型,差异有统计学意义(P <0.05)。结论成釉细胞瘤中自噬活性显著高于正常口腔黏膜组织,自噬过程在成釉细胞瘤的发生发展中发挥一定作用,可能对成釉细胞瘤的局部侵袭性有一定影响。
Objective To investigate the expression and significance of microtubule-associated protein 1 light chain 3(LC3),an autophagy marker protein,in ameloblastomas. Methods Immunohistochemical methods were employed to evaluate the expression of LC3 in104 cases of ameloblastomas and 20 cases of normal oral mucosal tissues. The results were analyzed by a semiquantitative analysis method. Results The reactivity of LC3 in the epithelial cells of ameloblastomas was positive,and the positivity rate was significantly higher than that in normal oral mucosal tissues(P < 0.05). There were no significant differences between the LC3 expression levels with respect to age,gender,or recurrence(P < 0.05). The positivity rate in mandible ameloblastomas was significantly higher than that in maxilla and gingiva ameloblastomas(P < 0.05). The reactivity for LC3 was significantly higher in solid ameloblastomas than that in the other three tissue types(P < 0.05). Conclusion Autophagic activity in ameloblastomas was higher than that in normal oral mucosal tissues. This suggests that autophagy plays an important role during tumorigenesis,and contributes to the local invasion of ameloblastomas.
Objective To investigate the expression and significance of microtubule-associated protein 1 light chain 3(LC3),an autophagy marker protein,in ameloblastomas. Methods Immunohistochemical methods were employed to evaluate the expression of LC3 in104 cases of ameloblastomas and 20 cases of normal oral mucosal tissues. The results were analyzed by a semiquantitative analysis method. Results The reactivity of LC3 in the epithelial cells of ameloblastomas was positive,and the positivity rate was significantly higher than that in normal oral mucosal tissues(P < 0.05). There were no significant differences between the LC3 expression levels with respect to age,gender,or recurrence(P < 0.05). The positivity rate in mandible ameloblastomas was significantly higher than that in maxilla and gingiva ameloblastomas(P < 0.05). The reactivity for LC3 was significantly higher in solid ameloblastomas than that in the other three tissue types(P < 0.05). Conclusion Autophagic activity in ameloblastomas was higher than that in normal oral mucosal tissues. This suggests that autophagy plays an important role during tumorigenesis,and contributes to the local invasion of ameloblastomas.
引文
[1]FREGNANI ER,DA CRUZ PEREZ DE,DE ALMEIDA OP,et al.Clinicopathological study and treatment outcomes of 121 cases of ameloblastomas[J].Int J Oral Maxillofac Surg,2010,39(2):145-149.DOI:10.1016/j.ijom.2009.11.022.
[2]NOBORU M,MASAAKI K.Autophagy:renovation of cells and tissues[J].Cell,2011,147(4):728-741.DOI:10.1016/j.cell.2011.10.026.
[3]OHSUMI Y.Molecular dissection of autophagy:two ubiquitin-like systems[J].Nat Rev Mol Cell Biol,2001,2(3):211-216.DOI:10.1038/35056522.
[4]MIZUSHIMA N.Autophagy:process and function[J].Genes Dev,2007,21(22):2861-2873.DOI:10.1101/gad.1599207.
[5]WHITE E,MEHNERT JM,CHAN CS.Autophagy,metabolism,and cancer[J].Clin Cancer Res,2015,21(22):5037-5046.DOI:10.1158/1078-0432.CCR-15-0490.
[6]王雅雯,侯劲松.自噬基因Beclin 1在肿瘤中的作用及其与口腔癌的关系[J].中国实用口腔科杂志,2011,4(6):374-376.DOI:10.3969/j.issn.1674-1595.2011.06.019.
[7]YANG J,WAN C,NIE S,et al.Localization of Beclin1 in mouse developing tooth germs:possible implication of the interrelation between autophagy and apoptosis[J].J Mol Histol,2013,44(6):619-627.DOI:10.1007/s10735-013-9518-3.
[8]YANG JW,ZHU LX,YUAN GH,et al.Autophagy appears during the development of the mouse lower first molar[J].Histochem Cell Biol,2013,139(1):109-118.DOI:10.1007/s00418-012-1016-2.
[9]LI RF,CHEN G,ZHAO Y,et al.Increased expression of autophagy-related proteins in keratocystic odontogenic tumours:its possible association with growth potential[J].Br J Oral Maxillofac Surg,2014,52(6):551-556.DOI:10.1016/j.bjoms.2014.03.009.
[10]SELVAMANI M,YAMUNADEVI A,BASANDI PS,et al.Analysis of prevalence and clinical features of multicystic ameloblastoma and its histological subtypes in South Indian sample population:a retrospective study over 13 years[J].J Pharm Bioallied Sci,2014,6(Suppl 1):S131-S134.DOI:10.4103/0975-7406.137419.
[11]MASTHAN KM,ANITHA N,KRUPAA J,et al.Ameloblastoma[J].J Pharm Bioallied Sci,2015,7(Suppl 1):S167-S170.DOI:10.4103/0975-7406.155891.
[12]DASH S,SARASHETTI PM,RAJASHEKAR B,et al.TGF-β2-induced EMT is dampened by inhibition of autophagy and TNF-αtreatment[J].Oncotarget,2018,9(5):6433-6449.DOI:10.18632/oncotarget.23942.
[13]HAO FY,LIU J,ZHONG M,et al.Expression of E-cadherin,vimentin andβ-catenin in ameloblastoma and association with clinicopathological characteristics of ameloblastoma[J].Int J Clin Exp Pathol,2018,11(1):199-207.
[14]PATSA S,JADAV RB,HALDER GC,et al.Demographic and histopathological variation of ameloblastoma:a hospital-based study[J].JOral Maxillofac Pathol,2016,20(2):230-233.DOI:10.4103/0973-029X.185937.
[15]ANTONOGLOU GN,S NDOR GK.Recurrence rates of intraosseous ameloblastomas of the jaws:a systematic review of conservative versus aggressive treatment approaches and meta-analysis of non-randomized studies[J].J Craniomaxillofac Surg,2015,43(1):149-157.DOI:10.1016/j.jcms.2014.10.027.
[2]NOBORU M,MASAAKI K.Autophagy:renovation of cells and tissues[J].Cell,2011,147(4):728-741.DOI:10.1016/j.cell.2011.10.026.
[3]OHSUMI Y.Molecular dissection of autophagy:two ubiquitin-like systems[J].Nat Rev Mol Cell Biol,2001,2(3):211-216.DOI:10.1038/35056522.
[4]MIZUSHIMA N.Autophagy:process and function[J].Genes Dev,2007,21(22):2861-2873.DOI:10.1101/gad.1599207.
[5]WHITE E,MEHNERT JM,CHAN CS.Autophagy,metabolism,and cancer[J].Clin Cancer Res,2015,21(22):5037-5046.DOI:10.1158/1078-0432.CCR-15-0490.
[6]王雅雯,侯劲松.自噬基因Beclin 1在肿瘤中的作用及其与口腔癌的关系[J].中国实用口腔科杂志,2011,4(6):374-376.DOI:10.3969/j.issn.1674-1595.2011.06.019.
[7]YANG J,WAN C,NIE S,et al.Localization of Beclin1 in mouse developing tooth germs:possible implication of the interrelation between autophagy and apoptosis[J].J Mol Histol,2013,44(6):619-627.DOI:10.1007/s10735-013-9518-3.
[8]YANG JW,ZHU LX,YUAN GH,et al.Autophagy appears during the development of the mouse lower first molar[J].Histochem Cell Biol,2013,139(1):109-118.DOI:10.1007/s00418-012-1016-2.
[9]LI RF,CHEN G,ZHAO Y,et al.Increased expression of autophagy-related proteins in keratocystic odontogenic tumours:its possible association with growth potential[J].Br J Oral Maxillofac Surg,2014,52(6):551-556.DOI:10.1016/j.bjoms.2014.03.009.
[10]SELVAMANI M,YAMUNADEVI A,BASANDI PS,et al.Analysis of prevalence and clinical features of multicystic ameloblastoma and its histological subtypes in South Indian sample population:a retrospective study over 13 years[J].J Pharm Bioallied Sci,2014,6(Suppl 1):S131-S134.DOI:10.4103/0975-7406.137419.
[11]MASTHAN KM,ANITHA N,KRUPAA J,et al.Ameloblastoma[J].J Pharm Bioallied Sci,2015,7(Suppl 1):S167-S170.DOI:10.4103/0975-7406.155891.
[12]DASH S,SARASHETTI PM,RAJASHEKAR B,et al.TGF-β2-induced EMT is dampened by inhibition of autophagy and TNF-αtreatment[J].Oncotarget,2018,9(5):6433-6449.DOI:10.18632/oncotarget.23942.
[13]HAO FY,LIU J,ZHONG M,et al.Expression of E-cadherin,vimentin andβ-catenin in ameloblastoma and association with clinicopathological characteristics of ameloblastoma[J].Int J Clin Exp Pathol,2018,11(1):199-207.
[14]PATSA S,JADAV RB,HALDER GC,et al.Demographic and histopathological variation of ameloblastoma:a hospital-based study[J].JOral Maxillofac Pathol,2016,20(2):230-233.DOI:10.4103/0973-029X.185937.
[15]ANTONOGLOU GN,S NDOR GK.Recurrence rates of intraosseous ameloblastomas of the jaws:a systematic review of conservative versus aggressive treatment approaches and meta-analysis of non-randomized studies[J].J Craniomaxillofac Surg,2015,43(1):149-157.DOI:10.1016/j.jcms.2014.10.027.