外源茉莉酸对桑天牛成虫取食及桑枝内抗虫次生物质含量和防御酶活性的影响
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摘要
茉莉酸作为信号分子在提高植物抗虫性方面起着重要作用。通过取食选择试验调查分别经3种浓度茉莉酸处理不同时间的桑枝被桑天牛成虫取食的面积差异,在此基础上测定分析茉莉酸处理桑枝内具有抗虫活性的次生物质含量及防御酶活性动态变化。用10、100、1 000μmol/L茉莉酸处理24 h或48 h后,桑枝被桑天牛成虫取食的面积与对照组无显著差异;处理72 h后,仅1 000μmol/L茉莉酸处理组桑枝被桑天牛成虫取食的面积明显低于对照组。Sperman等级相关性分析表明,茉莉酸浓度与桑天牛成虫的取食面积呈极显著负相关。用1 000μmol/L茉莉酸处理4~72 h,桑枝内多酚氧化酶(PPO)活性均明显高于对照,且在48 h时达到最大值;脂氧合酶(LOX)活性仅在处理8 h时显著提高;胰蛋白酶抑制剂(TI)活性和单宁含量随着处理时间的延长逐渐提高,但仅在72 h时显著高于对照组;1 000μmol/L茉莉酸处理可提高桑枝内总酚含量,在处理48 h时达到显著程度,但在72 h又降低至与对照组相当的水平。研究结果表明,1 000μmol/L茉莉酸处理桑枝具有阻碍桑天牛成虫取食的效果,并可诱导桑枝内抗虫次生物质含量及防御酶活性显著提高。
Jasmonic acid( JA),a defense signaling molecule,plays an important role in improving plant resistance against insects. Feeding choice test was employed to investigate the differences in the feeding area of Apriona germari adult on mulberry branches at different time points after treatment with three concentrations of JA. In addition,the dynamic changes of secondary metabolite content and defense enzyme activity in mulberry branches were measured after JA treatment. The feeding area of A. germari adult on mulberry branches,which were treated with 10,100 and 1 000 μmol/L JA for 24 and 48 h respectively,was not significantly different with that in control group. The feeding area of A.germari adult on mulberry branches treated with 1 000μmol/L JA for 72 h was significantly lower than that of the control. By Spearman's rank correlation analysis,a significant negative correlation between JA concentration and the feeding area of A. germari adult was found. Within 4 h to 72 h after treated with 1 000 μmol/L JA,the activities of polyphenol oxidase( PPO) in mulberry branches was significantly higher than that of the control,and reached the highest value at 48 h after treatment. The activity of lipoxygenase( LOX) in mulberry branches was significantly higher than that of the control only at 8 h after treatment. The trypsin inhibitor( TI) activity and tannin content in mulberry branches were gradually increased with treatment duration. However,they were significantly higher than those of the control only at 72 h after JA treatment. The content of total phenols in mulberry branches was increased after 1 000 μmol/L JA treatment. It was significantly higher than that of the control at 48 h after treatment,while decreased to the control level at 72 h after treatment. The results showed that 1 000 μmol/L JA was able to induce mulberry branches to display a significant antifeedant activity against A. germari adult and significant elevation of secondary metabolite content and defense enzyme activity.
Jasmonic acid( JA),a defense signaling molecule,plays an important role in improving plant resistance against insects. Feeding choice test was employed to investigate the differences in the feeding area of Apriona germari adult on mulberry branches at different time points after treatment with three concentrations of JA. In addition,the dynamic changes of secondary metabolite content and defense enzyme activity in mulberry branches were measured after JA treatment. The feeding area of A. germari adult on mulberry branches,which were treated with 10,100 and 1 000 μmol/L JA for 24 and 48 h respectively,was not significantly different with that in control group. The feeding area of A.germari adult on mulberry branches treated with 1 000μmol/L JA for 72 h was significantly lower than that of the control. By Spearman's rank correlation analysis,a significant negative correlation between JA concentration and the feeding area of A. germari adult was found. Within 4 h to 72 h after treated with 1 000 μmol/L JA,the activities of polyphenol oxidase( PPO) in mulberry branches was significantly higher than that of the control,and reached the highest value at 48 h after treatment. The activity of lipoxygenase( LOX) in mulberry branches was significantly higher than that of the control only at 8 h after treatment. The trypsin inhibitor( TI) activity and tannin content in mulberry branches were gradually increased with treatment duration. However,they were significantly higher than those of the control only at 72 h after JA treatment. The content of total phenols in mulberry branches was increased after 1 000 μmol/L JA treatment. It was significantly higher than that of the control at 48 h after treatment,while decreased to the control level at 72 h after treatment. The results showed that 1 000 μmol/L JA was able to induce mulberry branches to display a significant antifeedant activity against A. germari adult and significant elevation of secondary metabolite content and defense enzyme activity.
引文
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[22]越慧芳.外源茉莉酸诱导的青杨叶片保护酶活性变化及其对舞毒蛾幼虫生长发育的影响[D].呼和浩特:内蒙古农业大学,2013
[23]BHONWONG A,STOUT M J,ATTAJARUSIT J,et al.Defensive role of tomato polyphenol oxidases against cotton bollworm(Helicoverpa armigera)and beet armyworm(Spodoptera exigua)[J].J Chem Ecol,2009,35(1):28-38
[24]ZHOU G X,REN N,QI J F,et al.The 9-lipoxygenase Osr9-LOX1interacts with the 13-lipoxygenase-mediated pathway to regulate resistance to chewing and piercing-sucking herbivores in rice[J].Physiol Plant,2014,152(1):59-69
[25]崔伟康,杨鹏,廉法卓,等.茉莉酸信号转导途径介导番茄对棉铃虫的抗性[J].福建农林大学学报(自然科学版),2016,45(4):371-375
[26]KAROWE D N.Differential effect of tannic acid on two tree-feeding Lepidoptera:implications for theories of plant anti-herbivore chemistry[J].Oecologia,1989,80(4):507-512
[27]李继东,桑玉强,毕会涛,等.4树种树皮有机物质含量与对桑天牛抗性关系的研究[J].河南科学,2007,25(4):578-581
[2]WAR A R,PAULRAJ M G,IGNACIMUTHU S,et al.Induced resistance to Helicoverpa armigera through exogenous application of jasmonic acid and salicylic acid in groundnut,Arachis hypogaea[J].Pest Manag Sci,2015,71(1):72-82
[3]DELANEY K J,WAWRZYNIAK M,LEMAN'CZYK G,et al.Synthetic cis-jasmone exposure induces wheat and barley volatiles that repel the pest cereal leaf beetle,Oulema melanopus L.[J].J Chem Ecol,2013,39(5):620-629
[4]杨世勇,王蒙蒙,谢建春.茉莉酸对棉花单宁含量和抗虫相关酶活性的诱导效应[J].生态学报,2013,33(5):1615-1625
[5]MANDAL S.Induction of phenolics,lignin and key defense enzymes in eggplant(Solanum melongena L.)roots in response to elicitors[J].Afr J Biotechnol,2010,9(47):8038-8047
[6]宫玉艳,段立清,王爱清,等.外源茉莉酸诱导枸杞对枸杞蚜生长发育和繁殖的影响[J].昆虫学报,2010,53(6):670-674
[7]黄大庄,严俊杰,王志刚,等.桑天牛研究[M].北京:科学出版社,2015:9-10
[8]冯会藏,李继泉,卜志国,等.桑天牛危害和外源茉莉酸诱导桑树防御反应的研究[J].蚕业科学,2009,35(2):223-228
[9]李继泉,杨元,王树香,等.桑天牛卵长尾啮小蜂寄主选择定位行为[J].昆虫学报,2007,50(11):1122-1128
[10]桂连友.外源茉莉酸甲酯对茶树抗虫作用的诱导及其机理[D].杭州:浙江大学,2004
[11]姚锋先,曾晓春,蒋晓燕,等.水稻中以亚麻酸为底物的酯氧合酶活性测定[J].江西农业大学学报,2006,28(2):183-186
[12]王琪,严善春,王艳军.剪叶及昆虫取食对兴安落叶松蛋白酶抑制剂的影响[J].昆虫学报,2008,51(8):798-803
[13]孟昭军,周永泉,严善春,等.外源茉莉酸类化合物对2种落叶松针叶内单宁含量的影响[J].林业科学,2010,46(3):96-104
[14]冯远娇,王建武,骆世明.外源茉莉酸处理对Bt玉米直接防御物质含量及其相关基因表达的影响[J].中国农业科学,2009,40(11):2481-2487
[15]YANG Z W,DUAN X N,JIN S,et al.Regurgitant derived from the tea geometrid Ectropis obliqua suppresses wound-induced polyphenol oxidases activity in tea plants[J].J Chem Ecol,2013,39(6):744-751
[16]SENTHIL-NATHAN S,KALAIVANI K,CHOI M Y,et al.Effects of jasmonic acid-induced resistance in rice on the plant brownhopper,Nilaparvata lugens Stl(Homoptera:Delphacidae)[J].Pestic Biochem Physiol,2009,95(2):77-84
[17]田旭涛.茉莉酸诱导菜豆和番茄对美洲斑潜蝇的防御反应研究[D].杨凌:西北农林科技大学,2013
[18]张颖.外源茉莉酸诱导对枸杞瘿螨及其天敌的影响[D].呼和浩特:内蒙古农业大学,2012
[19]GUAN X M,LIU X X,LU Z Q,et al.Influence of tannic acid and Cry1Ac toxin of Bacillus thuringiensison larval suvival,growth and development of Helicoverpa armigera[J].Entomol Exp Appl,2009,132(1):50-58
[20]HEITZ T,BERGEY D R,RYAN C A.A gene encoding a chloroplast-targeted lipoxygenase in tomato leaves is transiently induced by wounding,systemin,and methyl jasmonate[J].Plant Physiol,1997,114(3):1085-1093
[21]AVDIUSHKO S A,BROWN G C,DAHLMAN D L,et al.Methyl jasmonate exposure induces insect resistance in cabbage and tobacco[J].Environ Entomol,1997,26(3):642-654
[22]越慧芳.外源茉莉酸诱导的青杨叶片保护酶活性变化及其对舞毒蛾幼虫生长发育的影响[D].呼和浩特:内蒙古农业大学,2013
[23]BHONWONG A,STOUT M J,ATTAJARUSIT J,et al.Defensive role of tomato polyphenol oxidases against cotton bollworm(Helicoverpa armigera)and beet armyworm(Spodoptera exigua)[J].J Chem Ecol,2009,35(1):28-38
[24]ZHOU G X,REN N,QI J F,et al.The 9-lipoxygenase Osr9-LOX1interacts with the 13-lipoxygenase-mediated pathway to regulate resistance to chewing and piercing-sucking herbivores in rice[J].Physiol Plant,2014,152(1):59-69
[25]崔伟康,杨鹏,廉法卓,等.茉莉酸信号转导途径介导番茄对棉铃虫的抗性[J].福建农林大学学报(自然科学版),2016,45(4):371-375
[26]KAROWE D N.Differential effect of tannic acid on two tree-feeding Lepidoptera:implications for theories of plant anti-herbivore chemistry[J].Oecologia,1989,80(4):507-512
[27]李继东,桑玉强,毕会涛,等.4树种树皮有机物质含量与对桑天牛抗性关系的研究[J].河南科学,2007,25(4):578-581