普洱茶对大鼠肥胖的干预和保肝护肝作用
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摘要
将SD大鼠随机分为正常对照组、高脂对照组、普洱茶低(0.45 g/kg)、中(0.90 g/kg)、高(1.35 g/kg)浓度组,每组10只,每天灌胃普洱茶,连续处理十二周后,禁食24 h,取大鼠肝脏,测其甘油三酯(TG)、总胆固醇(TC)的含量、腺苷酸活化蛋白激酶(AMPK)的表达及与脂代谢相关的基因表达,以研究普洱茶的预防肥胖和保肝护肝作用。结果显示,普洱茶能降低高脂饲料喂养的大鼠肝脏中TG和TC的含量,显著提高腺苷酸活化蛋白激酶(AMPK)磷酸化水平(p <0.05),极显著下调脂肪酸合成酶(FAS)、固醇调节元件结合蛋白1c(SREBP-1c)和羟甲基戊二酰辅酶A还原酶(HMGCR)的m RNA表达水平(p <0.01),作用效果呈剂量依赖性。因此,普洱茶具有良好的预防肥胖和保肝作用效果。
Sprague dawley(SD) rats were randomly divided into normal-diet control group,high-fat diet control group,and three levels groups(each group with 10 rats) treated with high(1.35 g/kg),medium(0.90 g/kg) and low(0.45 g/kg) hot water extracts from Pu-erh tea.The rats were lavaged every day for continuous administration for 12 weeks.After fasted for 24 h,the rats were sacrificed and the livers were used as materials.The contents of triglyceride(TG) and cholesterol(TC),the expression levels of phosphorylated adenosine monophosphate(AMP)-activated protein kinase(p-AMPK) protein and the m RNA related to lipid metabolism were determined for study Pu-erh tea's roles on intervention of obesity and protection of liver.The results showed that Pu-erh tea could reduce the contents of TG and TC,improving the level of AMPK phosphorylation(p < 0.05),up-regulating the m RNA levels of FAS,SREBP-1c and HMGCR(p < 0.01).The effects were in dose-dependent characteristics.Therefore,Pu-erh tea had good functions of preventing obesity and protecting liver.
Sprague dawley(SD) rats were randomly divided into normal-diet control group,high-fat diet control group,and three levels groups(each group with 10 rats) treated with high(1.35 g/kg),medium(0.90 g/kg) and low(0.45 g/kg) hot water extracts from Pu-erh tea.The rats were lavaged every day for continuous administration for 12 weeks.After fasted for 24 h,the rats were sacrificed and the livers were used as materials.The contents of triglyceride(TG) and cholesterol(TC),the expression levels of phosphorylated adenosine monophosphate(AMP)-activated protein kinase(p-AMPK) protein and the m RNA related to lipid metabolism were determined for study Pu-erh tea's roles on intervention of obesity and protection of liver.The results showed that Pu-erh tea could reduce the contents of TG and TC,improving the level of AMPK phosphorylation(p < 0.05),up-regulating the m RNA levels of FAS,SREBP-1c and HMGCR(p < 0.01).The effects were in dose-dependent characteristics.Therefore,Pu-erh tea had good functions of preventing obesity and protecting liver.
引文
[1]原剑,孙云波,周晓明,等.多肽抗体免疫富集-质谱法检测肝癌患者血清多肽标志物[J].分析化学,2011,39(8):1129-1133.
[2]曹海霞,范建高.脂肪性肝病:愈来愈严重的全球性公共卫生问题[J].胃肠病学,2011,16(3):129-130.
[3]Balaji M,Ganjayi M S,Hanuma kumar G E,et al.A review on possible therapeutic targets to contain obesity:the role of phytochemicals[J].Obesity Research&Clinical Practice,2015,10(4):363-380.
[4]Zeng L,Yan J,Luo L,et al.Effects of Pu-erh tea aqueous extract(PTAE)on blood lipid metabolism enzymes[J].Food&Function,2015,6(6):2008-2016.
[5]苏静静,王雪青,宋文军,等.普洱茶对小鼠血糖的干预作用[J].食品科学,2014,35(9):260-263.
[6]Su J J,Wang X Q,Song W J,et al.Reducing oxidative stress and hepatoprotective effect of the water extracts from Pu-erh tea on rats fed with high-fat diet[J].Food Science and Human Wellness,2016,5(4):199-206.
[7]Sun L J,Wang Y,Song Y,et al.Resveratrol restores the circadian rhythmic disorder of lipid metabolism induced by highfat diet in mice[J].Biochemical and Biophysical Research Communications,2015,458(1):86-91.
[8]孙晨光.论荷叶的减肥降脂作用[J].中医临床研究,2014,6(3):100-102.
[9]Cao Z H,Gu D H,Lin Q Y,et al.Effect of Pu-erh tea on body fat and lipid profiles in rats with diet-induced obesity[J].Phytotherapy Research PTR,2011,25(2):234-238.
[10]Hu W Y,Ma X H,Zhou W Y,et al.Preventive effect of Silibinin in combination with Pu-erh tea extract on non-alcoholic fatty liver disease in ob/ob mice[J].Food&Function,2017,8(3):1105-1115.
[11]Kuo K L,Weng M S,Chiang C T,et al.Comparative studies on the hypolipidemic and growth suppressive effects of oolong,black,pu-erh,and green tea leaves in rats[J].Journal of Agricultural&Food Chemistry,2005,53(2):480-489.
[12]谢振兴,李秀,耿旭,等.伽马氨基丁酸抑制高脂诱导肥胖小鼠肝脏氧化应激及肝脂肪变性[J].食品与生物技术学报,2015,33(6):613-620.
[13]肖鹏,白桦,栗敏,等.山茱萸提取物对大鼠原发性肝癌组织中B7-H6表达的影响[J].中国肿瘤临床,2017,44(22):1125-1129.
[14]杨伦韵,张晓艳,廉静,等.内参基因GAPDH和β-actin在BMP9诱导的骨髓间充质干细胞成骨分化过程中的表达情况[J].重庆医科大学学报,2015,40(4):546-549.
[15]Felder T K,Klein K,Patsch W,et al.A novel SREBP-1splice variant:tissue abundance and transactivation potency[J].Biochimica ET Biophysica ACTA(Bba)-Gene Structure and Expression,2005,1731(1):41-47.
[16]Reijo L,Thelen K M,Hannu P,et al.Genetic variant of the SREBF-1 gene is significantly related to cholesterol synthesis in man[J].Atherosclerosis,2006,185(1):206-209.
[17]刘姚,陈婷婷,傅凌韵,等.青钱柳多糖对高脂血症小鼠脂肪酸合成酶(FAS)表达影响[J].江西农业大学学报,2013,35(2):392-397.
[18]张谷,陈芝芸,严茂祥,等.大鼠非酒精性脂肪性肝病进展中肝脏脂代谢关键调控基因表达的变化[J].医学研究杂志,2013,42(5):157-160.
[19]Zhang B B,Zhou G C,Li C.Ampk:an emerging drug target for diabetes and the metabolic syndrome[J].Cell Metabolism,2009,9(5):407-416.
[20]蔡明春,黄庆愿,高钰琪.AMPK与能量代谢[J].重庆医学,2005(1):120-122.
[21]王会玲,李燕,胡伟锋,等.小檗碱影响AMPK/PGC-1信号途径改善糖尿病胰岛素抵抗和线粒体功能的研究[J].中华临床医师杂志(电子版),2014,8(5):896-900.
[22]陈彪,薛东芳,韩冰,等.黄连碱对胆固醇代谢关键基因的调节作用[J].中国中药杂志,2015,40(8):1548-1553.
[2]曹海霞,范建高.脂肪性肝病:愈来愈严重的全球性公共卫生问题[J].胃肠病学,2011,16(3):129-130.
[3]Balaji M,Ganjayi M S,Hanuma kumar G E,et al.A review on possible therapeutic targets to contain obesity:the role of phytochemicals[J].Obesity Research&Clinical Practice,2015,10(4):363-380.
[4]Zeng L,Yan J,Luo L,et al.Effects of Pu-erh tea aqueous extract(PTAE)on blood lipid metabolism enzymes[J].Food&Function,2015,6(6):2008-2016.
[5]苏静静,王雪青,宋文军,等.普洱茶对小鼠血糖的干预作用[J].食品科学,2014,35(9):260-263.
[6]Su J J,Wang X Q,Song W J,et al.Reducing oxidative stress and hepatoprotective effect of the water extracts from Pu-erh tea on rats fed with high-fat diet[J].Food Science and Human Wellness,2016,5(4):199-206.
[7]Sun L J,Wang Y,Song Y,et al.Resveratrol restores the circadian rhythmic disorder of lipid metabolism induced by highfat diet in mice[J].Biochemical and Biophysical Research Communications,2015,458(1):86-91.
[8]孙晨光.论荷叶的减肥降脂作用[J].中医临床研究,2014,6(3):100-102.
[9]Cao Z H,Gu D H,Lin Q Y,et al.Effect of Pu-erh tea on body fat and lipid profiles in rats with diet-induced obesity[J].Phytotherapy Research PTR,2011,25(2):234-238.
[10]Hu W Y,Ma X H,Zhou W Y,et al.Preventive effect of Silibinin in combination with Pu-erh tea extract on non-alcoholic fatty liver disease in ob/ob mice[J].Food&Function,2017,8(3):1105-1115.
[11]Kuo K L,Weng M S,Chiang C T,et al.Comparative studies on the hypolipidemic and growth suppressive effects of oolong,black,pu-erh,and green tea leaves in rats[J].Journal of Agricultural&Food Chemistry,2005,53(2):480-489.
[12]谢振兴,李秀,耿旭,等.伽马氨基丁酸抑制高脂诱导肥胖小鼠肝脏氧化应激及肝脂肪变性[J].食品与生物技术学报,2015,33(6):613-620.
[13]肖鹏,白桦,栗敏,等.山茱萸提取物对大鼠原发性肝癌组织中B7-H6表达的影响[J].中国肿瘤临床,2017,44(22):1125-1129.
[14]杨伦韵,张晓艳,廉静,等.内参基因GAPDH和β-actin在BMP9诱导的骨髓间充质干细胞成骨分化过程中的表达情况[J].重庆医科大学学报,2015,40(4):546-549.
[15]Felder T K,Klein K,Patsch W,et al.A novel SREBP-1splice variant:tissue abundance and transactivation potency[J].Biochimica ET Biophysica ACTA(Bba)-Gene Structure and Expression,2005,1731(1):41-47.
[16]Reijo L,Thelen K M,Hannu P,et al.Genetic variant of the SREBF-1 gene is significantly related to cholesterol synthesis in man[J].Atherosclerosis,2006,185(1):206-209.
[17]刘姚,陈婷婷,傅凌韵,等.青钱柳多糖对高脂血症小鼠脂肪酸合成酶(FAS)表达影响[J].江西农业大学学报,2013,35(2):392-397.
[18]张谷,陈芝芸,严茂祥,等.大鼠非酒精性脂肪性肝病进展中肝脏脂代谢关键调控基因表达的变化[J].医学研究杂志,2013,42(5):157-160.
[19]Zhang B B,Zhou G C,Li C.Ampk:an emerging drug target for diabetes and the metabolic syndrome[J].Cell Metabolism,2009,9(5):407-416.
[20]蔡明春,黄庆愿,高钰琪.AMPK与能量代谢[J].重庆医学,2005(1):120-122.
[21]王会玲,李燕,胡伟锋,等.小檗碱影响AMPK/PGC-1信号途径改善糖尿病胰岛素抵抗和线粒体功能的研究[J].中华临床医师杂志(电子版),2014,8(5):896-900.
[22]陈彪,薛东芳,韩冰,等.黄连碱对胆固醇代谢关键基因的调节作用[J].中国中药杂志,2015,40(8):1548-1553.