摘要
目的:通过腹腔注射全氟化碳(C8F18)对大鼠肺缺血再灌注损伤(lung ischemical reperfusion injury,LIRI)起到保护作用。方法:将健康清洁SD大鼠随机分空白组、对照组、实验组。建立大鼠LIRI模型。空白组(NC组)开胸不进行LIRI处理。对照组(NS组)术前48 h腹腔注射0.9%NS后行LIRI处理;实验组(PFC组)术前48 h腹腔注射C8F18后行LIRI处理。检测3组实验动物动脉血气分析、肿瘤坏死因子(TNF-α)、肺组织水通道蛋白(AQP1)及钠通道蛋白(ENaC)的表达。结果:NS组与PFC组2 h时间点比较PaCO2值差异有统计学意义(P <0.05),4 h、6 h时间点两组PaCO2值差异无统计学意义(P> 0.05)。PFC组与NS组各时间点比较全血TNF-α含量下降(P <0.05)。AQP1蛋白的表达以空白组最明显,NS组AQP1蛋白染色明显减少,以2h组最明显,各时间点与空白组比较均下降(P <0.05)。PFC组AQP1蛋白较NS组有显著增加(P <0.05)。ENaC蛋白表达以NC组最明显,NS组ENaC蛋白染色明显减少,各时间点与NC组比较均显著下降(P <0.05),PFC组ENaC蛋白较NS组无差异(P>0.05)。结论:全氟化碳大鼠腹腔注射后,在大鼠LIRI中动脉血Pa02增加,PaCO2下降,促进肺气体交换。使全血中TNF-α浓度下降,从而起到保护肺组织的作用。同时能增加肺组织AQP1、ENaC蛋白的表达,有效减轻肺水肿及炎症反应。
Objective: To investigate the protective effect of intraperitoneal injection of perfluorocarbon(C8 F18)on lung ischemia-reperfusion injury(LIRI) in rats. Methods: A total of 90 healthy and clean Sprague-Dawley rats were randomly divided into blank group, control group, and experimental group. A LIRI model was established in rats. The blank group(NC group) underwent thoracotomy without LIRI treatment. The control group(NS group) received intraperitoneal injection of 0.9% NS at 48 h before thoracotomy and LIRI treatment. The experimental group(PFC group) received intraperitoneal injection of C8 F18 at 48 h before thoracotomy and LIRI treatment. Arterial blood gas parameters and the expression of tumor necrosis factor(TNF-α), lung aquaporin(AQP1), and sodium channel protein(ENaC) were measured in the three groups. Results: There was a significant difference in partial pressure of carbon dioxide(PaCO2) at 2 h between the NS group and the PFC group(P < 0.05), but there were no significant differences in PaCO2 at 4 and 6 h between the two groups(P > 0.05). The PFC group had a significantly lower level of tumor necrosis factor-alpha(TNF-α) in whole blood at each time point compared with the NS group(P <0.05). Among the three groups, the NC group had the highest expression of AQP1. The staining of AQP1 in the NS group was significantly reduced, especially at 2 h, and the NS group had significantly lower expression of AQP1 at each time point compared with the NC group(P < 0.05). The PFC group had significant higher expression of AQP1 compared with the NS group(P < 0.05). Among the three groups, the NC group had the highest expression of ENaC.The staining of ENaC in the NS group was significantly reduced, and the NS group had significantly lower expression of ENaC at each time point compared with the NC group(P < 0.05). There was no significant difference in the expression of ENaC between the PFC group and the NS group(P > 0.05). Conclusion: In the LIRI rat model, intraperitoneal injection of perfluorocarbon has a protective effect on the lung tissue by promoting pulmonary gas exchange — increasing PaO2 in arterial blood and decreasing PaCO2 and by reducing the level of TNF-α in whole blood. At the same time, it can effectively alleviate pulmonary edema and inflammatory response by increasing the expression of AQP1 and ENaC in lung tissue.
引文
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