克罗恩病患者蛋白质组学差异表达的研究
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摘要
目的探讨克罗恩病患者血清中的差异表达蛋白。方法选取在本院诊治的克罗恩病患者20例,采用各种Cy Dve染料实施交叉标记后,依据顺序实施MALDI-TOF-MS、2-D DIGE等鉴定操作。结果对二者双向电泳图对比,发现克罗恩病患者在表达异常蛋白质点方面有多个,其中1 058蛋白质点,相比于正常成人组,则存在明显升高,即1.67倍(P<0.05),此蛋白质通过质谱鉴定得知,乃是一种结合珠蛋白质。结论在克罗恩病血清中,结合珠蛋白所存在的高表达,提示在其病程中免疫系统失衡可能存在一定作用。
Objective To investigate the differences in serum of patients with Crohn disease protein. Methods 20 cases in our hospital for treatment of Crohn's disease patients, the implementation of cross labeled by various Cy Dve dyes, the implementation of MALDI-TOF-MS based on2-D DIGE sequence identification operation. Results Of the two dimensional electrophoresis map comparison, found that patients with Crohn's disease the abnormal expression of protein spots are multiple, including 1 058 protein spots, compared to the normal adult group, there is increased 1.67times(P<0.05), the protein identification by mass spectrometry that is a combination of pearl protein in serum. Conclusion High expression of Crohn's disease, combined with the existing pearl protein, suggesting that in the course of immune system imbalance may have some effect.
Objective To investigate the differences in serum of patients with Crohn disease protein. Methods 20 cases in our hospital for treatment of Crohn's disease patients, the implementation of cross labeled by various Cy Dve dyes, the implementation of MALDI-TOF-MS based on2-D DIGE sequence identification operation. Results Of the two dimensional electrophoresis map comparison, found that patients with Crohn's disease the abnormal expression of protein spots are multiple, including 1 058 protein spots, compared to the normal adult group, there is increased 1.67times(P<0.05), the protein identification by mass spectrometry that is a combination of pearl protein in serum. Conclusion High expression of Crohn's disease, combined with the existing pearl protein, suggesting that in the course of immune system imbalance may have some effect.
引文
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[2]康亮,杨祖立,王磊,等.CD45在克罗恩病患者血清中的表达[J].南方医科大学学报,2009,29(2):259-263.
[3]陆晓春,韩树堂.肠内营养疗法在克罗恩病临床治疗中的应用进展[J].当代医学,2012,18(15):26-28.
[4]庞智.蛋白质组学在炎症性肠病分子机制研究中的应用前景[J].胃肠病学,2005,10(2):113-115.
[5]苏丽,李楠.蛋白质组学技术在炎症性肠病研究中的应用研究进展[J].中华消化病与影像杂志:电子版,2012,2(3):32-35.
[6]童明霞,缪应雷.蛋白质组学在炎症性肠病研究中的应用[J].世界华人消化杂志,2010(22):2333-2338.
[7]周强,张言斌,汤春梅,等.克罗恩病与肠结核鉴别诊断对比分析[J].当代医学,2009,15(6):38-39.
[8]翟宝进,刘欣民,李双英,等.颅内动脉瘤患者血清差异表达蛋白质的研究[J].中华神经外科杂志,2010,26(10):891-895.