人胎盘间充质干细胞复合多孔羟基磷灰石支架的体内异位成骨效果
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摘要
目的:探讨多孔羟基磷灰石(hydroxyapatite,HA)支架作为人工骨替代材料的异位成骨效果。方法:将人胚胎间充质干细胞与多孔HA支架混合培养7 d,取出细胞支架复合体移植入比格犬背肌内为实验组,同时植入未与细胞共培养的多孔HA支架于比格犬背肌内为对照组,在植入第12周时取出体内异位成骨组织进行成骨效果检测。结果:植入第12周时的异位成骨组织内均有新生血管形成,新生骨组织实验组明显多于对照组。结论:多孔HA支架在体内有很好的异位成骨能力,人胚胎间充质干细胞能促进新骨的形成。
Objective:To explore the ectopic osteogenesis effect of the porous hydroxyapatite(HA) scaffolds as the artificial bone substitute materials.Methods:Cultured human placenta derived mesenchymal stem cells with porous HA scaffolds for 7 d,cellscaffold composite were taken out to implanted in the beagle's back muscles as the experimental group,and implanted blanket porous HA scaffolds in the beagle's back muscles as the control group,respectively remove the ectopic osteogenesis organization at 12 weeks after implantation,then detected the osteogenesis effect of the ectopic ossification organizations.Results:Implanted at 12 weeks there had new blood supply in the ectopic ossification organizations,and the experimental group new bone tissue significantly greater than the control group.Conclusions:Porous HA ceramic scaffolds have good ectopic bone formation in vivo,and human placenta mesenchymal stem cells can promote the formation of new bone.
Objective:To explore the ectopic osteogenesis effect of the porous hydroxyapatite(HA) scaffolds as the artificial bone substitute materials.Methods:Cultured human placenta derived mesenchymal stem cells with porous HA scaffolds for 7 d,cellscaffold composite were taken out to implanted in the beagle's back muscles as the experimental group,and implanted blanket porous HA scaffolds in the beagle's back muscles as the control group,respectively remove the ectopic osteogenesis organization at 12 weeks after implantation,then detected the osteogenesis effect of the ectopic ossification organizations.Results:Implanted at 12 weeks there had new blood supply in the ectopic ossification organizations,and the experimental group new bone tissue significantly greater than the control group.Conclusions:Porous HA ceramic scaffolds have good ectopic bone formation in vivo,and human placenta mesenchymal stem cells can promote the formation of new bone.
引文
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[2]Arrabal PM,Visser R,Santos-Ruiz L,et al.Osteogenic molecules for clinical applications:improving the BMPcollagen System[J].Biol Res,2013,46(4):421-429.
[3]Sierra-Garcíal GD,Castro-Ríos R,Gónzalez-Hortal A,et al.Proteínas morfogenéticasóseas(BMP):aplicación clínica para reconstrucción de defectos en hueso[J].Gac Med Mex,2016,152(3):381-385.
[4]伍佳,温永梅,吕欣荣等.人胎盘来源间充质干细胞的生物学性能及体外生物相容性[J].中国组织工程研究,2017,21(5):755-759.[5〗Zhang HF,Mao XY,Zhao DY,et al.Three dimensional printed polylactic acid-hydroxyapatite composite scaffolds for prefabricating vascularized tissue engineered bone:An in vivo bioreactor model[J].Sci Rep,2017,7(1):15255.
[6]Caplan AI.New era of cell-based or thopedic therapies[J].Tissue Eng Part b Rev,2009,15(2):195-200.
[7]Banfi A,Muraglia A,Dozin B,et al.Proliferation kinetics anddifferentiation potential of ex vivo expanded human bone marrow stromal cells:Implications for their use in cell therapy[J].Exp Hematol,2001,28(6):707-715.
[8]Kern S,Eichler H,Stoeve J,et al.Comparative analysis of mesenchymal stem cells from bone marrow,umbilical cord blood,or adipose tissue[J].Stem Cells,2006,24(5):1294-1301.
[9]Kokubo T,and Takadama H.How useful is SBF in predicting in vivo bone bioactivity?[J].Biomaterials,2006,27(15):2907-2915.
[10]Vacanti C,Pietrzak WS.Musculoskeletal Tissue Regeneration:Biological Materials and Methods.New Jersey:Springer Science and Business Media,2008.
[11]Vacanti C,Pietrzak WS.Musculoskeletal Tissue Regeneration:Biological Materials and Methods[M].New Jersey:Springer Science and Business Media,2008.
[12]Miron RJ,Zhang YF.Osteoinduction:A review of old concepts with new standards[J].J Dent Res,2012,91(8):736-744.
[13]Rao RR,Stegemann JP.Cell-Based Approaches to the Engineering of Vascularized Bone Tissue[J].Cytotherapy,2013,15(11):1309-1322.
[14]Kaigler D,Krebsbach PH,Polverini PJ,et al.Transplanted endothelial cells enhance orthotopic bone regeneration[J].J Dent Res,2006,85(7):633-637.
[15]Kale S,Biermann S,Edwards C,et al.Three-dimensional cellular development is essential for ex vivo formation of human bone[J].Nat Biotechnol,2000,18(9):954-958.