异养细菌对含钒石煤微生物浸出效果的研究
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摘要
研究了混合异养细菌对含钒石煤的微生物浸出效果,并采用X荧光半定量(XRF)、X射线衍射(XRD)和傅里叶红外光谱分析(FTIR)等检测手段对石煤原矿、细菌浸出后残渣进行了矿物结构的表征。研究结果表明,混合异养细菌可以浸出含钒石煤,浸出29 d后,上清液中Al,Si和V的最高质量浓度分别达到14.63,55.14和26.95 mg·L~(-1);浸出结束后,V的浸出率达26.87%,且通过添加新鲜培养液,钒的浸出率可提高至37.97%;经混合异养细菌作用后,石煤中云母结构中层间水的氢键发生了变化,即其结构中阳离子发生变化,且590 cm~(-1)处的Si-O-M弯曲振动峰消失。
The bioleaching of vanadium-bearing stone coal by mixed heterotrophic bacteria was studied. And the detection methods of X-ray fluorescence( XRF),X-ray diffraction( XRD) and Fourier transform infrared spectroscopy( FTIR) were used to analyze the mineral structure of ore stone coal and the residue. The results showed that the ions of vanadium-bearing stone coal could be leached out by mixed heterotrophic bacteria. The highest concentration of Al,Si and V were 14. 63,55. 14,and 26. 95 mg·L~(-1) after 29 d,respectively,and the leaching rate of V was 26. 87%. The leaching rate of V could be increased to 37. 97% by adding fresh medium.The bioleaching activity caused the changes of hydrogen bonding of interlayer water in muscovite( the changes of interlayer cation) and the disappearance of Si-O-M bending vibration bands in 590 cm~(-1).
The bioleaching of vanadium-bearing stone coal by mixed heterotrophic bacteria was studied. And the detection methods of X-ray fluorescence( XRF),X-ray diffraction( XRD) and Fourier transform infrared spectroscopy( FTIR) were used to analyze the mineral structure of ore stone coal and the residue. The results showed that the ions of vanadium-bearing stone coal could be leached out by mixed heterotrophic bacteria. The highest concentration of Al,Si and V were 14. 63,55. 14,and 26. 95 mg·L~(-1) after 29 d,respectively,and the leaching rate of V was 26. 87%. The leaching rate of V could be increased to 37. 97% by adding fresh medium.The bioleaching activity caused the changes of hydrogen bonding of interlayer water in muscovite( the changes of interlayer cation) and the disappearance of Si-O-M bending vibration bands in 590 cm~(-1).
引文
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[18]Zhang Y J,Li X H,Pan L P,Wei Y S.Effect of mechanical activation on the kinetics of extracting indium from indium-bearing zinc ferrite[J].Hydrometallurgy,2010,102(1):95.
[19]Zhu Y,Cao W Z,Lu A H,Wang Q H,Li Y,Zhang X L,Wang C Q.A study of interaction between montmorillonite and a strain of Bacillus mucilaginosus[J].Acta Petrologica Et Mineralogica,2011,30(1):121.(朱云,曹维政,鲁安怀,王清华,李艳,张虓雷,王长秋.胶质芽孢杆菌-蒙脱石相互作用实验研究[J].岩石矿物学杂志,2011,30(1):121.)
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[2]Moskalyk R R,Alfantazi A M.Processing of vanadium:a review[J].Minerals Engineering,2003,16(9):793.
[3]Zhang Y M,Bao S X,Liu T,Chen T J,Huang J.The technology of extracting vanadium from stone coal in China:history,current status and future prospects[J].Hydrometallurgy:2011,109(1):116.
[4]Zhou X Y,Li C L,Li J,Liu H Z,Wu S Y.Leaching of vanadium from carbonaceous shale[J].Hydrometallurgy,2009,99:97.
[5]Hu P C,Zhang Y M,Liu T,Huang J,Yuan Y Z,Yang Y D.Separation and recovery of iron impurity from a vanadium-bearing stone coal via an oxalic acid leachingreduction precipitation process[J].Separation and Purification Technology,2017,180:99.
[6]Hu Y J,Zhang Y M,Bao S X,Liu T.Effects of the mineral phase and valence of vanadium on vanadium extraction from stone coal[J].International Journal of Minerals,Metallurgy and Materials,2012,19(10):893.
[7]Zeng X,Wang F,Zhang H F,Cui J J,Yu J,Xu G W.Extraction of vanadium from stone coal by roasting in a fluidized bed reactor[J].Fuel,2015,142:180.
[8]Wang X W,Wang M Y.State of art development of vanadium extraction from stone coal,Iron steel vanadium titanium,2012,33(1):8.(王学文,王明玉.石煤提钒工艺现状及发展趋势[J].钢铁钒钛,2012,33(1):8.)
[9]Feng X S,Ye L Y,Wang X F,Qian Z S.Study on the bioleaching of vanadium-bearing stone coal by Thiobacillus ferrooxidans and Thiobacillus thiooxidans[J].Journal of Zhejiang Agricultural University,1981,7(2):99.(冯孝善,叶立扬,王献凤,钱泽澍.氧化硫硫杆菌或氧化铁硫杆菌溶浸含钒石煤的研究[J].浙江农业大学学报,1981,7(2):99.)
[10]Zhang X Z,Lin H,Sun D S,Zhang M.Structural effects of silicate minerals on the growth metabolism and desilicification of a strain of silicate bacterium[J].Journal of Chongqing University,2014,37(5):98.(张贤珍,林海,孙德四,张敏.硅酸盐结构对硅酸盐细菌生长代谢及脱硅的影响[J].重庆大学学报,2014,37(5):98.)
[11]Sun D S,Zhang X Z,Xiao G G.Bioleaching of richpotassium igneous rock by potassium-solubilizing culture and change of bacterial community structure during leaching process[J].Journal of Central South University(Science and Technology),2014,45(9):2941.(孙德四,张贤珍,肖国光.“钾”细菌浸出富钾火成岩及细菌群落结构的变化[J].中南大学学报(自然科学版),2014,45(9):2941.)
[12]Wang Q L,Liu X M.Effects of p H and temperature on Thiobacillus Ferrooxidans'oxidation of Fe2+[J].Mining and Metallurgical Engineering,2004,24(2):36.(王清良,刘选明.p H值与温度对氧化亚铁硫杆菌氧化Fe2+影响的研究[J].矿业工程,2004,24(2):36.)
[13]Yun X,Wu P X,Zhu N W,Zhang T,Liu W,Wu J H,Li P.Bioleaching of copper from waste printed circuit boards by bacterial consortium enriched from acid mine drainage[J].Journal of Hazardous Materials,2010,184:812.
[14]Hu H F,Cheng P,Wang X X,Jie X L,Li Q M.Dissolution of citric acid,oxalic acid and malic acid on kaolinite[J].Chinese Journal of Soil Science,2013,44(3):635.(胡华峰,程璞,王兴祥,介晓磊,李清曼.柠檬酸、草酸和苹果酸对高岭石的溶解作用[J].土壤通报,2013,44(3):635.)
[15]Zhou Y F,Wang R C,Lu X C,Chen T H.Roles of adhered paenibacillus polymyxa in the dissolution and flotation of bauxite:a dialytic investigation[J].Frontiers of Earth Science in China,2010,4(2):167.
[16]Kong M M,Zheng S X,Huang L,Liu F.The change of some layered silicate minerals in the culture of low molecular weight organic acids[J].Soils,2013,45(2):285.(孔明明,郑世学,黄丽,刘凡.低分子量有机酸培养下几种层状硅酸盐矿物的变化[J].土壤,2013,45(2):285.)
[17]Sun D S,Wang H J,Zhang Q.Effects of Bacillus circulans on decomposition behavior of bauxite[J].The Chinese Journal of Nonferrous Metals,2013,23(4):1119.(孙德四,王化军,张强.环状芽孢杆菌对铝土矿浸出分解行为的影响[J].中国有色金属学报,2013,23(4):1119.)
[18]Zhang Y J,Li X H,Pan L P,Wei Y S.Effect of mechanical activation on the kinetics of extracting indium from indium-bearing zinc ferrite[J].Hydrometallurgy,2010,102(1):95.
[19]Zhu Y,Cao W Z,Lu A H,Wang Q H,Li Y,Zhang X L,Wang C Q.A study of interaction between montmorillonite and a strain of Bacillus mucilaginosus[J].Acta Petrologica Et Mineralogica,2011,30(1):121.(朱云,曹维政,鲁安怀,王清华,李艳,张虓雷,王长秋.胶质芽孢杆菌-蒙脱石相互作用实验研究[J].岩石矿物学杂志,2011,30(1):121.)
[20]Liu J,Zhang Y M,Huang J,Liu T,Ma H.Extracting vanadium from stone coal by mechanical activation equipment[J].Chinese Journal of Rare Metals,2015,39(6):554.(刘娟,张一敏,黄晶,刘涛,马浩.机械活化设备对石煤提钒浸出的影响[J].稀有金属,2015,39(6):554.)