摘要
研究了青蛤(Cyclina sinensis)养殖环境中异养细菌数量和弧菌数量的动态变化,以探讨其与养殖密度的相关性和样本间相关性。结果表明:实验周期内各养殖塘水体中异养细菌总数呈现不同的变化趋势且差异不显著(P> 0. 05),而弧菌数量变化趋势较为一致,9月份与其他月份水样中弧菌数量差异较为显著(P<0. 05)。各塘底泥中异养细菌总数变化和弧菌数量变化差异均不显著,而不同密度养殖塘底泥中弧菌数量变化差异显著(P <0. 05)。不同采样时间同一塘的青蛤腔液异养细菌总数差异极显著(P <0. 01),而弧菌数量差异较为显著(P <0. 05)。样本间、样本与养殖密度间相关性分析发现,水体中异养细菌总数变化与弧菌数量变化呈显著正相关(r=0. 335,P <0. 05);底泥中异养细菌总数变化与弧菌数量变化呈正相关且相关性极显著(r=0. 587,P <0. 01);底泥和水体中异养细菌总数和弧菌数量变化与养殖密度相关性均不显著(P>0. 05);青蛤腔液异养细菌总数变化与养殖密度相关性极显著(r=-0. 189,P <0. 01),弧菌数量变化与养殖密度相关性极显著(r=-0. 123,P <0. 01)。
To study the association between the dynamics of heterotrophic bacteria and the density of rearing Cyclina sinensis,and to provide the basis information to assessment and predict the mariculture pond environment,heterotrophic bacteria of rearing water,sediment and Cyclina sinensis cavity fluid were collected from May to December in Wenling seashore,Zhejiang. The results showed that the quantity dynamics of Vibrio in rearing water were significantly different among ponds( P < 0. 05),but the quantity dynamics of heterotrophic bacteria were not. In addition,no significant differences were observed in heterotrophic bacteria number in sediment among ponds,but the changes of Vibrio number presented significant differences between different ponds( P < 0. 05). Significant difference of heterotrophic bacteria quantity were observed in Cyclina sinensis cavity fluid among samples from the same pond but col-lected at different time( P < 0. 01),and similar results was observed in the Vibrio quantity( P < 0. 05). The changes of heterotrophic bacteria and Vibrio number in water showed a significant positively correlation( r = 0. 335,P <0. 05),while they showed a significant positively correlation( r = 0. 587,P < 0. 01) in sediment samples; but there were no significant correlation between culture density and heterotrophic bacteria and Vibrio in water and sediment;Culture density and the changes of heterotrophic bacteria number in Cyclina sinensis showed a significant negative correlation( r =-0. 189,P < 0. 01),and culture density and the changes of Vibrio number in Cyclina sinensis cavity fluid showed a significant negative correlation( r =-0. 123,P < 0. 01).
引文
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