异常汉逊酵母和植物乳杆菌菌液浓度与光密度值的关系研究
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摘要
[目的]探索一种快速测定异常汉逊酵母和植物乳杆菌菌液浓度的方法。[方法]采用分光光度法测定菌液的光密度值(OD值),平板菌落计数法测定异常汉逊酵母和植物乳杆菌的菌液浓度,并研究两者的相关关系。[结果]异常汉逊酵母在YEPD培养基中指数期的菌液浓度与光密度值呈现极显著的线性相关关系,菌液浓度(y)与光密度值(x)线性回归方程:y=2.246x-0.030(R2=0.998 6)。植物乳杆菌在MRS肉汤培养基中指数期菌液浓度的对数值与光密度值呈现极显著的线性相关关系,菌液浓度的对数值(y)与光密度值(x)线性回归方程:y=0.172 4x+8.432 1(R~2=0.996 6)。[结论]分光光度法可用于快速测定异常汉逊酵母和植物乳杆菌菌液浓度。
[Objective] To explore a rapid measurement for bacteria concentration of Hansenula anomala and Lactobacillus plantarum.[Method] Spectrophotometry and plate counting were used to determine the optical density( OD) and bacteria fluid concentration respectively,investigate the correlations between them.[Result] The correlations of the optical density with bacteria concentration were well.Bacterial concentration( y) and optical density( x) linear regression equation was established of Hansenula anomala YEPD medium in logarithmic phase:y = 2.246x-0.030( R2= 0.998 6).The logarithm of bacterial concentration( y) and optical density( x) linear regression equation was established of Lactobacillus plantarum MRS medium in logarithmic phase: y = 0.172 4x + 8.432 1( R2= 0.996 6).[Conclusion] Spectrophotometric method can be used for rapid determination of Hansenula anomala and Lactobacillus plantarum bacteria fluid concentration.
[Objective] To explore a rapid measurement for bacteria concentration of Hansenula anomala and Lactobacillus plantarum.[Method] Spectrophotometry and plate counting were used to determine the optical density( OD) and bacteria fluid concentration respectively,investigate the correlations between them.[Result] The correlations of the optical density with bacteria concentration were well.Bacterial concentration( y) and optical density( x) linear regression equation was established of Hansenula anomala YEPD medium in logarithmic phase:y = 2.246x-0.030( R2= 0.998 6).The logarithm of bacterial concentration( y) and optical density( x) linear regression equation was established of Lactobacillus plantarum MRS medium in logarithmic phase: y = 0.172 4x + 8.432 1( R2= 0.996 6).[Conclusion] Spectrophotometric method can be used for rapid determination of Hansenula anomala and Lactobacillus plantarum bacteria fluid concentration.
引文
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[2]董自艳,戴翚,马仕洪,等.紫外-可见分光光度法快速确定细菌菌液的浓度[J].中国药品标准,2014,15(2):120-121.
[3]王改玲,宋云杰,高竞铎,等.畜牧业中4种常用有益菌浓度与吸光度的关系[J].江苏农业科学,2016,44(7):274-276.
[4]马勇,樊永军.用OD值监测产油酵母培养过程中的菌体生物量变化[J].安徽农业科学,2011,39(12):7342-7343.
[5]马勇,季祥,蔡禄.2种测定产油酵母菌生长曲线方法的比较[J].安徽农业科学,2011,39(14):8202-8203,8233.
[6]严佩峰,张孔海,李建芳.乳酸菌培养液中活菌数与吸光度的关系研究[J].信阳农业高等专科学校学报,2012,22(1):110-112.