摘要
为获得产高分子质量聚唾液酸(polysialic acid,PSA)菌株,分步采用常温常压等离子体(atmospheric and room temperature plasma,ARTP)和硫酸二乙酯(diethyl sulfate,DES)对大肠杆菌K235进行诱变。结果表明,通过ARTP诱变筛选得到1株产PSA分子质量较初始菌株提高了36. 84%的E. coli K235 4B31。对E. coli K235 4B31进行DES诱变,获得1株产PSA分子质量较初始菌株提高了78. 18%的突变菌株E. coli K235 6E61。通过三阶段搅拌转速控制策略最终使得突变菌株E. coli K235 6E61发酵产物PSA分子质量达到430. 5 k Da,为目前已报道的最高分子质量PSA。
In order to produce polysialic acid( PSA) with high molecular weight,Escherichia coli K235 was treated by atmospheric and room temperature plasma( ARTP) and diethyl sulfate( DES). Mutant 4 B31 obtained by ARTP mutagenesis produced PSA with 36. 84% higher molecular weight than that of the parent strain. Mutant 6 E61 obtained from mutant 4 B31 by DES mutagenesis produced PSA with 78. 18% higher molecular weight. By using a threestage agitation speed fermentation,the maximal molecular weight of PSA produced by mutant 6 E61 was 430. 5 kDa,which was the highest value reported up to date.
引文
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