甘草叶斑病菌对戊唑醇和异菌脲的敏感性
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摘要
[目的]明确甘草叶斑病菌对戊唑醇和异菌脲的敏感现状,评价甘草叶斑病菌对戊唑醇和异菌脲的抗性风险。[方法]2016—2017年从甘草病叶上分离获得22株甘草叶斑病菌,采用菌丝生长速率法分别测定了各菌株对戊唑醇和异菌脲的敏感性。[结果]22株甘草叶斑病菌对戊唑醇的EC_(50)在0.442 6～2.201 2μg/mL,平均EC_(50)为(1.180 2±0.426 7)μg/mL,其对戊唑醇的敏感性呈单峰分布,可作为将来进行田间甘草叶斑病对戊唑醇抗药性检测的敏感性基线,抗性指数为0.375 0～1.865 1。22株甘草叶斑病菌对异菌脲的EC_(50)在0.096 4～1.639 6μg/mL,EC_(50)的平均值为(0.352 7±0.329 8)μg/mL,其对异菌脲的敏感性呈单峰分布,可作为将来进行田间甘草叶斑病对异菌脲抗药性检测的敏感性基线,抗性指数为0.273 3～4.648 7,菌株WW17-16的抗性指数为4.648 7,属于低抗菌株。[结论]所有供试甘草叶斑病菌株对戊唑醇是敏感的,而对异菌脲已有产生抗药性的趋势,生产中需引起高度重视。
[Objective]The aim was to investigate the sensitivity of A.azukiae and assess the resistance risk of A.azukiae to tebuconazole and iprodione.[Method] The sensitivities to tebuconazole and iprodione of the 22 strains of A. azukiae collected from disease foliage from 2016 to 2017 were determined with the method of colonial growth-rate.[Result] The EC_(50) values of these isolates to tebuconazole ranged from 0. 442 6 to2.201 2 μg/m L,with the mean value of( 1.180 2±0.426 7) μg/m L,the sensitivity of these isolates appeared as a single peak curve,which could be used as the relative baseline sensitivity of A.azukiae to tebuconazole.The resistance index ranged from 0.375 0 to 1.865 1.The EC_(50) values of these isolates to iprodione ranged from 0.096 4 to 1.639 6 μg/m L,with the mean value of( 0.352 7±0.329 8) μg/m L,the sensitivity of these isolates appeared as a single peak curve,which could be used as the relative baseline sensitivity of A.azukiae to iprodione.The resistance index ranged from 0.273 3 to 4.648 7.The resistance index of strain WW17-16 was 4.648 7,which belonged to low resistance isolate.[Conclusion]All the tested A.azukiae isolates are sensitive to tebuconazole,while have been generated trend of resistance to iprodione and more attention needed to be paid in production.
[Objective]The aim was to investigate the sensitivity of A.azukiae and assess the resistance risk of A.azukiae to tebuconazole and iprodione.[Method] The sensitivities to tebuconazole and iprodione of the 22 strains of A. azukiae collected from disease foliage from 2016 to 2017 were determined with the method of colonial growth-rate.[Result] The EC_(50) values of these isolates to tebuconazole ranged from 0. 442 6 to2.201 2 μg/m L,with the mean value of( 1.180 2±0.426 7) μg/m L,the sensitivity of these isolates appeared as a single peak curve,which could be used as the relative baseline sensitivity of A.azukiae to tebuconazole.The resistance index ranged from 0.375 0 to 1.865 1.The EC_(50) values of these isolates to iprodione ranged from 0.096 4 to 1.639 6 μg/m L,with the mean value of( 0.352 7±0.329 8) μg/m L,the sensitivity of these isolates appeared as a single peak curve,which could be used as the relative baseline sensitivity of A.azukiae to iprodione.The resistance index ranged from 0.273 3 to 4.648 7.The resistance index of strain WW17-16 was 4.648 7,which belonged to low resistance isolate.[Conclusion]All the tested A.azukiae isolates are sensitive to tebuconazole,while have been generated trend of resistance to iprodione and more attention needed to be paid in production.
引文
[1]阎合.甘草链格孢叶斑病研究[D].兰州:甘肃农业大学,2009.
[2]曹雪梅.甘草根腐病病原学研究及室内药剂筛选[D].杨凌:西北农林科技大学,2013.
[3]李学斌,陈林,李国旗,等.中国甘草资源的生态分布及其繁殖技术研究[J].生态环境学报,2013,22(4):718-722.
[4]阎合,徐秉良,梁巧兰,等.甘草叶斑病的发生与病原菌鉴定[J].植物保护,2009,35(3):111-114.
[5]王婧,翟伟卜,高环,等.链格孢引起的病害严重危害农作物生产并危及农产品安全[J].植物保护,2017,43(4):9-15.
[6]何美仙,符雨诗,阮若昕,等.柑橘链格孢褐斑病菌对4种新型杀菌剂敏感性评价[J].浙江大学学报(农业与生命科学版),2016,42(5):535-542.
[7]姚亮亮,张铉哲,赵宇.黑龙江省分离的马铃薯早疫病病菌对腐霉利和戊唑醇的敏感性分析[J].作物杂志,2011(3):29-33.
[8]兰波,李湘民,黄瑞荣,等.江西省稻瘟病菌对富士一号的抗药性研究[J].江西农业大学学报,2007,29(3):351-355.
[9]袁杰,杨学辉,何海永,等.贵州省稻瘟病菌对三环唑敏感性研究[J].西南农业学报,2006,19(2):219-221.
[10]胡健,杨静雅,李婕,等.草坪草币斑病菌对甲基硫菌灵、异菌脲和丙环唑的敏感性[J].农药学学报,2017,19(6):694-700.
[11]陈帅民,芦帆,张璨,等.北京地区草莓灰霉病菌对异菌脲的抗性及抗性分子机制[J].植物保护,2015,41(5):100-104.
[12]潘以楼,朱桂梅,郭建.江苏草莓灰霉病菌对5种杀菌剂的抗药性[J].江苏农业学报,2013,29(2):299-304.
[13]任璐,史晓晶,姚众,等.苹果斑点落叶病菌对戊唑醇敏感基线建立及抗性突变体适合度[J].植物病理学报,2017,47(3):380-388.
[14]范昆,曲健禄,李林光,等.苹果轮纹病菌对戊唑醇的敏感基线及其室内抗药突变体研究[J].果树学报,2013,30(4):650-656.
[15]范昆,付丽,李晓军,等.苹果轮纹病菌对戊唑醇的敏感性及其抗性突变体的致病力[J].植物保护,2017,43(1):140-147.
[16]MA Z H,MORGAN D P,FELTS D,et al.Sensitivity of Botryosphaeria dothidea from California pistachio to tebuconazole[J].Crop protection,2002,21(9):829-835.
[17]顾春波,姜莉莉,王开运,等.抗戊唑醇草莓枯萎病菌ZY-W的诱导及其生物学特性[J].中国农业科学,2010,43(14):2897-2905.
[2]曹雪梅.甘草根腐病病原学研究及室内药剂筛选[D].杨凌:西北农林科技大学,2013.
[3]李学斌,陈林,李国旗,等.中国甘草资源的生态分布及其繁殖技术研究[J].生态环境学报,2013,22(4):718-722.
[4]阎合,徐秉良,梁巧兰,等.甘草叶斑病的发生与病原菌鉴定[J].植物保护,2009,35(3):111-114.
[5]王婧,翟伟卜,高环,等.链格孢引起的病害严重危害农作物生产并危及农产品安全[J].植物保护,2017,43(4):9-15.
[6]何美仙,符雨诗,阮若昕,等.柑橘链格孢褐斑病菌对4种新型杀菌剂敏感性评价[J].浙江大学学报(农业与生命科学版),2016,42(5):535-542.
[7]姚亮亮,张铉哲,赵宇.黑龙江省分离的马铃薯早疫病病菌对腐霉利和戊唑醇的敏感性分析[J].作物杂志,2011(3):29-33.
[8]兰波,李湘民,黄瑞荣,等.江西省稻瘟病菌对富士一号的抗药性研究[J].江西农业大学学报,2007,29(3):351-355.
[9]袁杰,杨学辉,何海永,等.贵州省稻瘟病菌对三环唑敏感性研究[J].西南农业学报,2006,19(2):219-221.
[10]胡健,杨静雅,李婕,等.草坪草币斑病菌对甲基硫菌灵、异菌脲和丙环唑的敏感性[J].农药学学报,2017,19(6):694-700.
[11]陈帅民,芦帆,张璨,等.北京地区草莓灰霉病菌对异菌脲的抗性及抗性分子机制[J].植物保护,2015,41(5):100-104.
[12]潘以楼,朱桂梅,郭建.江苏草莓灰霉病菌对5种杀菌剂的抗药性[J].江苏农业学报,2013,29(2):299-304.
[13]任璐,史晓晶,姚众,等.苹果斑点落叶病菌对戊唑醇敏感基线建立及抗性突变体适合度[J].植物病理学报,2017,47(3):380-388.
[14]范昆,曲健禄,李林光,等.苹果轮纹病菌对戊唑醇的敏感基线及其室内抗药突变体研究[J].果树学报,2013,30(4):650-656.
[15]范昆,付丽,李晓军,等.苹果轮纹病菌对戊唑醇的敏感性及其抗性突变体的致病力[J].植物保护,2017,43(1):140-147.
[16]MA Z H,MORGAN D P,FELTS D,et al.Sensitivity of Botryosphaeria dothidea from California pistachio to tebuconazole[J].Crop protection,2002,21(9):829-835.
[17]顾春波,姜莉莉,王开运,等.抗戊唑醇草莓枯萎病菌ZY-W的诱导及其生物学特性[J].中国农业科学,2010,43(14):2897-2905.