冷暴露机体肢端血氧饱和度作为寒冷损伤预警生物学指标的探索研究
|
摘要
目的为维护寒区部队官兵作业能力,探索肢端血氧饱和度作为寒冷环境应激损伤实时有效预警技术指标的可行性。方法利用环境模拟舱对大鼠施加1～3 h-15℃的寒冷刺激,观测冷应激大鼠后肢内侧体表温度、血管一氧化氮合酶(nitric oxide synthase,NOS)活性、肢端血氧饱和度的变化,采用Pearson相关系数评价肢端血氧饱和度与冷应激反应的相关性。结果大鼠接受1～3 h寒冷刺激后,后肢内侧体表温度、血管NOS活性及肢端血氧饱和度均明显下降,且肢端血氧饱和度与冷应激大鼠血管NOS活性、后肢内侧体表温度的变化密切相关。结论冷应激过程中大鼠肢端血氧饱和度与后肢内侧体表温度和血管NOS活性高度相关,可作为冷应激损伤的预警标志,对肢端血氧饱和度的监测可为冷应激损伤提供一种实时预警途径。
Objective To investigate the possibility that saturation of blood oxygen serves as a candidate parameter for cold injury warning in cold exposure organisms. Methods Cold stress(-15℃ for 1 or 3 h) was administered on rats with the simulation cabin. Groin temperature,saturation of blood oxygen and vascular NOS activity were detected after cold stress. Pearson association analysis was used for exploring the relationships between parameters. Results The groin temperature,saturation of blood oxygen,and vascular NOS activity decreased significantly in cold exposure rats.Positive correlations were found between saturation of blood oxygen and groin temperature or NOS activity. Conclusion Saturation of blood oxygen varies with skin temperature and vascular NOS activity in cold stress,suggesting that it can be used as a candidate real-time parameter for early warning of cold injury.
Objective To investigate the possibility that saturation of blood oxygen serves as a candidate parameter for cold injury warning in cold exposure organisms. Methods Cold stress(-15℃ for 1 or 3 h) was administered on rats with the simulation cabin. Groin temperature,saturation of blood oxygen and vascular NOS activity were detected after cold stress. Pearson association analysis was used for exploring the relationships between parameters. Results The groin temperature,saturation of blood oxygen,and vascular NOS activity decreased significantly in cold exposure rats.Positive correlations were found between saturation of blood oxygen and groin temperature or NOS activity. Conclusion Saturation of blood oxygen varies with skin temperature and vascular NOS activity in cold stress,suggesting that it can be used as a candidate real-time parameter for early warning of cold injury.
引文
[1]陈仁升,康尔泗,吴立宗,等.中国寒区分布探讨[J].冰川冻土,2005,27(4):469-475.
[2]于宁.冷环境中机体肾上腺素水平对血管内皮细胞NOS活性的影响及其在冷适应建立中的意义[D].北京:中国人民解放军军事医学科学院,2006.
[3]Moran DS,Heled Y,Shani Y,et al.Hypothermia and local cold injuries in combat and no-combat situations--the Israeli experience[J].Aviat Space Environ Med,2003,74(3):281-284.
[4]陈建国,林乐泓.脉搏肢端血氧饱和度监测过程中的影响因素的分析[J].现代医学与健康研究电子杂志,2018,2(8):141-143.
[5]谷万里,史载祥,贾海忠,等.可视光肢端血氧饱和度评价寒凝血瘀心肌缺血大鼠微循环的实验研究[J].微循环学杂志,2010,20(2):80-80.
[6]黄冠鹏,王基野,陈迎雷,等.21 d间歇性冷刺激对大鼠耐寒能力的影响及机制[J].实用预防医学,2012,19(5):658-661.
[7]张锴,李积胜.寒冷环境对机体的影响及其机制[J].国外医学卫生学分册,2006,33(4):212-215.
[8]Mills WJ Jr,O'Malley J,Kappes B.Cold and freezing:a historical chronilogy of laboratory investigation and clinical experience[J].Alaska Med,1993,35(1):89-116.
[9]郑晓慧.冷应激对动脉粥样硬化不稳定斑块的影响及机制研究[D].西安:第四军医大学,2012.
[10]晨阳,薛康,李春跃.原发性高血压与应激性高血压大鼠NO/NOS、ET的变化[J].中国医学物理学杂志,2006,23(1):35-39.
[11]崔玉英,唐朝枢,耿彬.束缚应激大鼠血小板及血管内膜的L-精氨酸/一氧化氮合酶/一氧化氮通路下调[J].北京大学学报(医学版),2006,38(3):231-235.
[12]吉宏龙,李天麟,秦汝莉,等.冷水浸手对手指皮温及血管机能的影响[J].职业医学,1995,32(6):9-10.
[13]姜在福,张是敬.部队人员冷习服程度的评价[J].解放军预防医学杂志,1995,13(1):1-4.
[14]钱令嘉,潘宁,弓景波,等.缺氧冷损伤血管一氧化氮合酶活性的变化及其生物学意义[J].中华预防医学杂志,2001,35(1):58-61.
[15]钱令嘉,潘宁,吴小红,等.冷损伤血管NO合酶活力的变化及其生物学意义[J].中华劳动卫生职业病杂志,1999,17(5):25-28.
[16]于宁,杨成君,尹旭辉,等.冷暴露大鼠血浆肾上腺素及血管内皮细胞NOS活性的变化[J].解放军预防医学杂志,2006,24(5):321-323.
[17]杨成君,王灿,赵肃,等.冷暴露对大鼠血清中NO、NOS含量的影响[J].职业与健康,2009,25(10):1042-1043.
[18]Mc Ewen BS,Bowles NP,Gray JD,et al.Mechanisms of stress in the brain[J].Nat Neurosci,2015,18(10):1353-1363.
[2]于宁.冷环境中机体肾上腺素水平对血管内皮细胞NOS活性的影响及其在冷适应建立中的意义[D].北京:中国人民解放军军事医学科学院,2006.
[3]Moran DS,Heled Y,Shani Y,et al.Hypothermia and local cold injuries in combat and no-combat situations--the Israeli experience[J].Aviat Space Environ Med,2003,74(3):281-284.
[4]陈建国,林乐泓.脉搏肢端血氧饱和度监测过程中的影响因素的分析[J].现代医学与健康研究电子杂志,2018,2(8):141-143.
[5]谷万里,史载祥,贾海忠,等.可视光肢端血氧饱和度评价寒凝血瘀心肌缺血大鼠微循环的实验研究[J].微循环学杂志,2010,20(2):80-80.
[6]黄冠鹏,王基野,陈迎雷,等.21 d间歇性冷刺激对大鼠耐寒能力的影响及机制[J].实用预防医学,2012,19(5):658-661.
[7]张锴,李积胜.寒冷环境对机体的影响及其机制[J].国外医学卫生学分册,2006,33(4):212-215.
[8]Mills WJ Jr,O'Malley J,Kappes B.Cold and freezing:a historical chronilogy of laboratory investigation and clinical experience[J].Alaska Med,1993,35(1):89-116.
[9]郑晓慧.冷应激对动脉粥样硬化不稳定斑块的影响及机制研究[D].西安:第四军医大学,2012.
[10]晨阳,薛康,李春跃.原发性高血压与应激性高血压大鼠NO/NOS、ET的变化[J].中国医学物理学杂志,2006,23(1):35-39.
[11]崔玉英,唐朝枢,耿彬.束缚应激大鼠血小板及血管内膜的L-精氨酸/一氧化氮合酶/一氧化氮通路下调[J].北京大学学报(医学版),2006,38(3):231-235.
[12]吉宏龙,李天麟,秦汝莉,等.冷水浸手对手指皮温及血管机能的影响[J].职业医学,1995,32(6):9-10.
[13]姜在福,张是敬.部队人员冷习服程度的评价[J].解放军预防医学杂志,1995,13(1):1-4.
[14]钱令嘉,潘宁,弓景波,等.缺氧冷损伤血管一氧化氮合酶活性的变化及其生物学意义[J].中华预防医学杂志,2001,35(1):58-61.
[15]钱令嘉,潘宁,吴小红,等.冷损伤血管NO合酶活力的变化及其生物学意义[J].中华劳动卫生职业病杂志,1999,17(5):25-28.
[16]于宁,杨成君,尹旭辉,等.冷暴露大鼠血浆肾上腺素及血管内皮细胞NOS活性的变化[J].解放军预防医学杂志,2006,24(5):321-323.
[17]杨成君,王灿,赵肃,等.冷暴露对大鼠血清中NO、NOS含量的影响[J].职业与健康,2009,25(10):1042-1043.
[18]Mc Ewen BS,Bowles NP,Gray JD,et al.Mechanisms of stress in the brain[J].Nat Neurosci,2015,18(10):1353-1363.